AbstractOn a geomagnetic quiet night of October 29, 2018, we captured an observational evidence of the onset of dark band structures within the field‐of‐view of an all‐sky airglow imager operating at 630.0 nm over a geomagnetic low‐mid latitude transition region, Hanle, Leh Ladakh. Simultaneous ionosonde observations over New Delhi shows the occurrence of spread‐F in the ionograms. Additionally, virtual and peak height indicate vertical upliftment in the F layer altitude and reduction in the ionospheric peak frequency were also observed when the dark band pass through the ionosonde location. All these results confirmed that the observed depletions are indeed associated with ionospheric F region plasma irregularities. The rate of total electron content index (ROTI) indicates the absence of plasma bubble activities over the equatorial/low latitude region which confirms that the observed event is a mid‐latitude plasma depletion. Our calculations reveal that the growth time of the plasma depletion is ∼2 h if one considers only the Perkins instability mechanism. This is not consistent with the present observations as the plasma depletion developed within ∼25 min. By invoking possible Es layer instabilities and associated E‐F region coupling, we show that the growth rate increases roughly by an order of magnitude. This strongly suggests that the Cosgrove and Tsunoda mechanism may be simultaneously operational in this case. Furthermore, it is also suggested that reduced F region flux‐tube integrated conductivity in the southern part of onset region created conducive background conditions for the growth of the plasma depletion on this night.