The ventilation performance of airborne infection isolation rooms (AIIRs) was assessed in three Finnish hospitals by examining the air change rate, contaminant removal efficiency and leakage of contaminants outside the isolation room by using tracer gas techniques. Results showed that infectious agents can escape from the AIIR during egress despite high ventilation rates in the AIIR and anteroom (air change rate, 4–24 h−1) and the pressure difference between the AIIR and corridor was −0.2 to −29 Pa. The control of impurities was often ineffective due to inappropriate direction of air flows and air moving from the patient towards the health care worker and anteroom. Although an anteroom reduces leakage of infectious agents to the corridor significantly, it does not prevent this completely when healthcare workers move between the AIIR and corridor. To enhance the protection of AIIRs, it is especially important to pay attention to the air distribution and removal efficiency of impurities in AIIRs and anterooms. Performance of AIIRs should be tested regularly, especially among older AIIRs.