ObjectiveTo explore the experiences of neonatal nurses in the implementation of a tool to enhance relationships between staff and parents in the neonatal unit: the You and Your Baby Nursery Guide. DesignQualitative descriptive design with focus groups. SettingThe study took place in a Level 4, 20-bed neonatal unit in Melbourne, Victoria, Australia. ParticipantsPurposive sample of seven registered nurses who worked day or afternoon shifts. MethodsWe conducted two semistructured focus groups after a 4-week implementation period of the You and Your Baby Nursery Guide. Participants completed a weekly reflective journal throughout the implementation period. We audiotaped and transcribed the focus groups and qualitatively analyzed the interview data with the use of thematic analysis. ResultsUse of the guide helped transform the relationships between parents and staff. The use of the guide enhanced communication, promoted participants’ personal reflection on their clinical skills and style/approach to parent engagement, and directly affected the care participants provided to infants and families. ConclusionThe You and Your Baby Nursery Guide was a useful resource to facilitate the delivery of family-centered, developmentally supportive care.