Adverse Growing Conditions Research Articles

Эколого-биологические методы формирования устойчивых лесомелиоративных комплексов в степных и полупустынных ландшафтах европейской территории России

The arid region of the European territory of Russia includes a zone of dry steppe (dark chestnut soils) and a semi-desert zone (light chestnut and brown soils). There is practically no woody natural vegetation in the region on elevations and plains, and on the prevailing complexes of zonal soils, forest plantations are characterized by low durability. Materials and methods. The work used the results of field (expedition) and laboratory studies of the growth, condition, durability, productivity of protective forest plantations of tree and shrub species in various soil and hydrological conditions of the dry-steppe and semi desert zones. Results and discussion. The author’s developments made it possible to recommend a reliable range of trees and shrubs for the creation of forest-reclamation plantations in each agroforestry area and soil ecological niche. It was revealed that when selecting the assortment, a significant role should be given to selection and genetic methods of using seed and planting material well adapted to local conditions (drought-, salt- and frost-resistant and healthy genotypes). The main reason for the early drying of protective plantations on complex light chestnut soils of the dry-steppe and semi-desert zones has been established. Taking into account the extremely arid conditions and the main causes of their drying, the concept of increasing their stability and effectiveness in adverse growing conditions is given. Agroforestry zoning and groups of soil forest suitability by type and classification, as well as groups of longevity of the main tree species are given. Conclusion. The large diversity of soils contributes to the formation of a stepped profile in adult cereals. Woody species shrink when their root system comes into contact with salt horizons. Ecological techniques are reduced to the correct selection of tree species that correspond to a specific ecological background. The technological use of differentiated agricultural cultivation techniques involves the use of moisture-saving technologies. Biological measures include biological reclamation of unsuitable soils and methods of breeding and genetic improvement of the qualitative composition of forest reclamation plantations. Suggestions for practical applications and directions for future research. The results of the work can be used by managers and specialists of forestry enterprises, researchers, graduate students, students of educational institutions. The assortment of trees and shrubs can be used in the design of plantings of various types on different soils.

PLANTING OF STIMULATED CORN SEEDS AT DIFFERENT DATES IN SPRING SEASONS

This study was aimed to investigate the appropriate planting date and tolerance to adverse growing conditions in the spring season through seeds stimulation technology. A field experiment was carried out in the spring seasons of 2022 and 2023 according to a randomized complete block design in a split-plot arrangement with four replications. The main factor is planting dates (February 15th, March 1st and 15th, and April 1st and 15th). The secondary factor is seeds stimulation (potassium nitrate 6 mg L-1 + licorice extract 6 g L-1, as well as soaking with distilled water only). The stimulated seeds (potassium nitrate + licorice extract) and the planting date of February 15th were superior in traits of fertility ratio, number of grains per ear, weight of 300 grains, total grains yield, biological yield, and harvest index in both seasons (2022 and 2023). The stimulated seeds (potassium nitrate + licorice extract), planted on February 15th, excelled in the traits above (95.6 and 97.7%), (640.7 and 816.9 grain ear-1), (76.19 and 82.69 g), (7.2 and 7.8 ton ha-1), (17.5 and 18.9 ton ha-1) and (41.5 and 41.2%) in both seasons, respectively. It can be concluded that seeds stimulation improved the yield and its components of corn crop under a wide range of environmental conditions, and that planting the stimulated seeds on February 15th contributed to take advantage of the prevailing environmental conditions and regulating the relationship between source efficiency and sink capacity to obtain the highest grain yield. It can be recommended that soaking the seeds with potassium nitrate 6 mg L-1 + licorice extract 6 g L-1 together when planting in mid-February in the spring season to increase grains yield.

Plants from Arid and Semi-Arid Zones of Mexico Used to Treat Respiratory Diseases: A Review.

Medicinal plants have been a traditional remedy for numerous ailments for centuries. However, their usage is limited due to a lack of evidence-based studies elucidating their mechanisms of action. In some countries, they are still considered the first treatment due to their low cost, accessibility, and minor adverse effects. Mexico is in second place, after China, in inventoried plants for medicinal use. It has around 4000 species of medicinal plants; however, pharmacological studies have only been carried out in 5% of its entirety. The species of the Mexican arid zones, particularly in semi-desert areas, exhibit outstanding characteristics, as their adverse growing conditions (e.g., low rainfall and high temperatures) prompt these plants to produce interesting metabolites with diverse biological activities. This review explores medicinal plants belonging to the arid and semi-arid zones of Mexico, focusing on those that have stood out for their bioactive potential, such as Jatropha dioica, Turnera diffusa, Larrea tridentata, Opuntia ficus-indica, Flourensia cernua, Fouquieria splendes, and Prosopis glandulosa. Their extraction conditions, bioactive compounds, mechanisms of action, and biological efficacy are presented, with emphasis on their role in the treatment of respiratory diseases. Additionally, current research, novel applications, and perspectives concerning medicinal plants from these zones are also discussed.

Fine-control of growth and thermotolerance in plant response to heat stress

Global warming impacts plant growth and development, which in turn threatens food security. It has become clear that plants how to response warm-temperature (such as thermomorphogenesis) and high-temperature stress. At the molecular level, many small molecules play crucial roles in balancing growth and defense, achieving high and stable yields by fine-tuning in response to external stimuli. Therefore, it is essential to understand the molecular mechanisms underlying how plants grow in response to heat stress and how they can adjust their biological processes to survive heat stress conditions. In this review, we summarized the heat-responsive genetic networks in plants and crop plants based on recent studies. Focus on how plants sense the elevated temperatures and initiate cellular and metabolic responses that enable them to adapt to the adverse growing conditions. We also describe the trade-off between plant growth and responses to heat stress. Specifically, we address the regulatory network of plant response to the heat stress, which will facilitate the discovery of novel thermotolerant genes and be helpful to open new opportunities for agricultural applications.

Exploring rhizobacteria of wild sugarcane (Saccharum spontaneum L.) and their plant growth promoting potential

In this study, the rhizospheric soil of wild sugarcane from three different salt-affected soils of the eastern Indo-Gangetic Plains was sampled. Rhizospheric bacteria from S. spontaneum were isolated and they were characterized for growth-promoting traits with molecular identification. A total of 49 isolates were isolated from three S. spontaneum rhizosphere soils from different places. These isolates were shown to have differential tolerance to NaCl concentrations up to 2000 mM. Owing to their host habitat and poor nutrient availability, these bacteria were found to have excellent tolerance to high NaCl concentrations along with having multiple plant growth traits. Apart from salt tolerance, these isolates were screened for EPS production, phosphate solubilization, zinc solubilization, siderophore production and ammonia production and based on multiple plant growth-promoting traits, twenty isolates were taken for molecular identification and further in vivo screening. These twenty S. spontaneum rhizospheric microbes belonged to the classes Gammaproteobacteria, Firmicutes (Bacilli) and Actinomycetia, representing six genera and 15 species. In vivo growth promotion studies indicated that some of these isolates could improve germination and growth in rice seedlings. The results of this study signify the possibility of utilizing wild crop microbiomes to nurture cultivated crops under adverse growing conditions.

Interaction of Phytohormones and External Environmental Factors in the Regulation of the Bud Dormancy in Woody Plants.

Bud dormancy and release are essential phenomena that greatly assist in adapting to adverse growing conditions and promoting the holistic growth and development of perennial plants. The dormancy and release process of buds in temperate perennial trees involves complex interactions between physiological and biochemical processes influenced by various environmental factors, representing a meticulously orchestrated life cycle. In this review, we summarize the role of phytohormones and their crosstalk in the establishment and release of bud dormancy. External environmental factors, such as light and temperature, play a crucial role in regulating bud germination. We also highlight the mechanisms of how light and temperature are involved in the regulation of bud dormancy by modulating phytohormones. Moreover, the role of nutrient factors, including sugar, in regulating bud dormancy is also discussed. This review provides a foundation for enhancing our understanding of plant growth and development patterns, fostering agricultural production, and exploring plant adaptive responses to adversity.

Sustained xanthophyll pigments-related photoprotective NPQ is involved in photoinhibition in the haptophyte Tisochrysis lutea

Dynamic xanthophyll cycle (XC) related non-photochemical quenching (NPQd, also called qE) is present in most phototrophs. It allows dissipating excess light energy under adverse growing conditions. Generally, NPQd rapidly reverses for photosynthesis to resume when light intensity decreases back toward optimal intensity. Under certain environmental conditions and/or in some species, NPQ can be strongly sustained (NPQs showing hours-to-days relaxation kinetics). Tisochrysis lutea is a South Pacific haptophyte phytoplankton with a strong potential for aquaculture and biotechnology applications. It was previously reported to show a surprisingly low NPQd capacity while synthesizing large amounts of diatoxanthin (Dt), a pigment involved in the XC. In order to better understand this paradox, we investigated the characteristics of NPQ in T. lutea under various growth conditions of light and nutrient availability (different photoperiods, low and high light, nutrient starvations). We found a strong NPQs, unmeasurable with usual fluorometry protocols. Along with confirming the involvement of Dt in both NPQd and NPQs (by using the dithiothreitol inhibitor), we highlighted a strong relationship between Dt and the maximum quantum yield of photochemistry (Fv/Fm) across growing conditions and during relaxation experiments in darkness. It suggests that changes in Fv/Fm, usually attributed to the ‘photoinhibitory’ quenching (qI), are simultaneously largely impacted by photoprotective NPQ. The overlap of xanthophyll pigments-related photoprotective NPQ with several other mechanisms involved in the cell response (Photosystem II photoinactivation, changes in pigments composition, and detoxification by antioxidants) to energy unbalance is further discussed. Our findings question both how widespread NPQs is in the global ocean, particularly in nutrient starved environments (oligotrophic waters) and situations (post-bloom), and the use of adapted active fluorescence protocols (i.e. with extended NPQ relaxation period prior to measurement).

Frost rings as time markers in Northern Hemisphere tree-ring chronologies, with special reference to the 1627 BC and AD 536 events

This paper discusses the findings of frost rings in three supra-long Northern Hemisphere tree-ring chronologies. Comparison between recently published data representing bristlecone pine from western North America, Scots pine from northern Fennoscandia and Siberian larch and Siberian spruce from the Yamal Peninsula shows the rare occurrence of frost rings dated to exactly same calendar years in more than one of these chronologies. The frost ring dates evident in all three datasets are dated to 1627 BC and AD 536. Previously, tree-ring features indicative of adverse growing conditions around 1627 BC have been discussed in the literature. Here, it is emphasised that the frost rings 1627 BC and AD 536 are dated to exactly same calendar year in all available tree-ring records, which demonstrates the value of such an event as a definitive time marker. Other major events of frost rings from multiple sites were dated to 421 BC, 251 BC, AD 627, AD 884, AD 985, AD 1109, AD 1259, AD 1453, AD 1601 and AD 1884. Similar to 1627 BC and AD 536, some of these events have been attributed to volcanic forcing and climate anomalies that led to human consequences and disasters. The case of the 1650 s BC appears different, with possibility of more than one major eruptions, as suggested by frost ring events dated to 1656 BC and 1653 BC in Yamal and western USA, respectively.

Early selection for drought tolerance in popcorn based on gene effects estimated in seedlings.

Low rainfall rates are becoming increasingly frequent because of climate change, causing droughts and threatening world food security. For popcorn, drought is the most limiting abiotic factor for plant's growth and development. Thus, the water deficit directly impacts for crop productivity. Based on knowledge of the genetic basis of traits involved in stages of popcorn germination and seedling development under water stress, genotypes with potential for adaptation to adverse growing conditions can be selected early. Therefore, data on genetic effects and combining ability of 10 popcorn parents were compiled to propose breeding strategies for the development of cultivars with greater adaptation to water stress in the early stages. Forty-five diallel hybrids were evaluated under two different water regimes, that is, water stress and full irrigation. This corresponded to a water retention capacity of 25% and 70% of the germination paper. The plants were watered daily as needed for seven days. A range of factors were evaluated, that is, germination traits including the germination speed index and germination on the seventh day; shoot traits including length and dry weight; and root system including length, dry weight, root-to-shoot ratio, maximum root number, root network area, specific and root network length, and root volume. Breeding for drought adaption in the early stages of popcorn development can be successful when hybrids are used, because of the genetic effects of dominance (ϕs). These control the traits evaluated at the seedling stage. The combinations L61 x P2 and L71 x P3 were recommended, in view of the more successful performance estimated for traits related to the shoot and root system.

Parental enriched environment: A look through key temporary windows.

Environmental enrichment is a widely used experimental manipulation that physically, cognitively and socially stimulates individuals. It has a great variety of long-term effects at neuroanatomical, neurochemical, and behavioral level; however, the influence of parental environmental enrichment during gestation and pregestation on the development of the offspring and on the mother's behavior has been poorly explored. This article presents a review of the literature from the year 2000 about the effects of maternal and paternal environmental enrichment on the behavioral, endocrine, and neural systems of offspring and parents. Relevant research terms were searched for on the biomedical databases, PubMed, Medline, ScienceDirect, and Google Scholar. The data suggest that paternal/maternal environmental enrichment can profoundly affect the developmental trajectories of offspring through putative epigenetic mechanisms. Environmental enrichment presents as a promising therapeutic tool for human health interventions, especially to counteract the deleterious effects of impoverished and adverse growing conditions.

Yield and related traits of three legume crops grown in olive-based agroforestry under an intense drought in the South Mediterranean

Heat and drought stresses have become more frequent and intense in the Mediterranean, strongly influencing arable crop phenology, growth, and grain yield. Agroforestry systems can effectively buffer the adverse climate conditions and stabilize or even increase crop yield under climate change. However, the positive effects of agroforestry remain uncertain due to the possible intense competition between trees and crops, especially for legume crops that have been less studied than cereals in such context. This study aimed to assess the response of the phenology, growth, grain yield, and yield-related traits of chickpea (Cicer arietinum), faba bean (Vicia faba), and lentil (Lens culinaris) to olive-based agroforestry (AFS) as compared to sole crops system (SCS) in the South of the Mediterranean. We conducted a field experiment during two growing seasons marked by an intense drought, either at the beginning (year 1) or at the end (year 2) of the crop cycle. Crop growth and yield were lower in year 1 than in year 2, reflecting the adverse growing conditions caused by the early drought. They were also lower in AFS than in SCS for both years, indicating that trees had competitive effects on crops. In year1, the yield loss of grains in AFS was 66 % for lentil, 47 % for chickpea, and 43 % for faba beancompared to SCS, confirming the greater shade sensitivity of lentil. In year 2, the reduction was significantly smaller and was about 46 %, 34 %, 38 % for lentil, chickpea and, faba bean, respectively. The number of pods and grains were the most affected yield components by agroforestry and drought timing across the three legumes crops. Similar responses were found when comparing crops at differentdistances to trees within the AFS field. Crops generally had lower biomass and yield, explained by fewer pods and grains, on the northern side of trees compared to the southern side of trees or the middle of tree inter-rows, causing significant spatial heterogeneity in crops. However, lentil and chickpea had apositive response to shade during the early drought year while a negative response during the late drought year, suggesting that the benefits of the microclimate created by olive trees express dependingon drought timing and crop physiology. Our study supports legume integration into AFS, suggestingthat chickpea should be considered during high-stress conditions, while faba bean should be preferredduring low-stress conditions.

Нові перспективні сорти агрусу (Ribes uva-crispa L.) селекції Інституту садівництва НААН України

The results of gooseberry selection work in IS NAAS are presented. The described varieties are entered in the "State Register of Varieties…". All studied varieties have different colors, from white, white-yellow green to red and purple, different ripening periods from very early to late, high taste and marketable qualities. At the same time, modern climatic risks, which increase the abiotic impact on garden crops, in particular gooseberries, and lead to a decrease in productivity, require constant updating of the assortment with a higher adaptability to adverse growing conditions without loss in economic productivity. A relevant and effective measure to solve this problem is scientifically based selection and creation of gooseberry varieties with high profitability of this crop/ The described varieties are included in the "State Register of Varieties..." and are undergoing the last stages of field research for distinction, uniformity and stability. All varieties are highly transportable, universal purpose. The described varieties of gooseberry are suitable for creating intensive, energy-saving plantations of ecological direction. They can also be used in selection work as a source of specific economically valuable traits, namely: different ripening periods, different colors, high yield, resistance to fungal diseases, and high taste and marketable characteristics. Key words: gooseberry, variety, productivity, selection, resistance, fruits, bran.

Increasing potato yield using foliar fertilization to boost growth

To date, the use of fertilizers has become firmly embedded in advanced crop cultivation technologies, as the main component of obtaining high and sustainable yields. The nutritional regime of plants cannot be optimized only with the help of macronutrients. They also need trace elements that can increase the resistance of plants to adverse growing conditions, diseases and pests. However, the high cost of such fertilizers makes it necessary to develop new more effective and less expensive drugs. Therefore, in modern potato cultivation technologies, much attention is paid to non-root top dressing. As a result of three-fold leaf treatments with the developed fertilizer, the yield increase of potato variety Gulliver was 0.4…5.2 t/ha, variety Vimpel 2.0…5.1 t/ha, variety Matushka 0.1…4.1 t/ha.

Canonical and Noncanonical ER Stress-Mediated Autophagy Is a Bite the Bullet in View of Cancer Therapy

Cancer cells adapt multiple mechanisms to counter intense stress on their way to growth. Tumor microenvironment stress leads to canonical and noncanonical endoplasmic stress (ER) responses, which mediate autophagy and are engaged during proteotoxic challenges to clear unfolded or misfolded proteins and damaged organelles to mitigate stress. In these conditions, autophagy functions as a cytoprotective mechanism in which malignant tumor cells reuse degraded materials to generate energy under adverse growing conditions. However, cellular protection by autophagy is thought to be complicated, contentious, and context-dependent; the stress response to autophagy is suggested to support tumorigenesis and drug resistance, which must be adequately addressed. This review describes significant findings that suggest accelerated autophagy in cancer, a novel obstacle for anticancer therapy, and discusses the UPR components that have been suggested to be untreatable. Thus, addressing the UPR or noncanonical ER stress components is the most effective approach to suppressing cytoprotective autophagy for better and more effective cancer treatment.

ASSESSMENT OF VARIETY SAMPLES OF COMMON BEANS ACCORDING TO THE VARIABILITY OF SIGNS

In the course of research, varieties of common bean were selected, which were distinguished by stable indicators of the duration of the growing season, which depended to a lesser extent on the hydrothermal regime of the conditions of the years of research. This will contribute to the formation of a stable growing season in dry and optimal years of research in terms of moisture supply. The highest coefficients of repeatability were noted in varieties of common bean: UD0301786 - 0.98, with a coefficient of variation (V) - 1%; UD0302656 – 0.97, coefficient of variation – 1%; UD0300495 – 0.95, coefficient of variation – 1.45%; UD0302223 – 0.95, and the coefficient of variation – 1.34%; UD0302398 – 0.95, coefficient of variation – 1.33%; UD0300019 is 0.95, and the coefficient of variation is 1.3%. The weight of 1,000 grains affects the grain productivity, while, as already noted, varieties with low variability of the weight of 1,000 grains belong to drought-resistant ones. Higher values of the mass of 1000 grains compared to the standard and, at the same time, lower variability according to statistical indicators were identified in the variety samples: UD0300577 – 284.7 g, repeatability coefficient – 0.9, variation coefficient – 7%; UD0303753 – 274.5 g, repeatability coefficient – 0.9, coefficient of variation – 7.1%; UD0300004 – 265.4 g, repeatability coefficient – 0.86, coefficient of variation – 9%; UD0303398 – 243.4 g, repeatability coefficient – 0.91, coefficient of variation – 6.0%; UD0302256 – 234.6 g, repeatability coefficient – 0.9, coefficient of variation – 8.4%; Yield is a summary feature, the level and stability of which are affected by resistance to diseases and pests, drought resistance, heat resistance, cold resistance, and manufacturability.Higher yield and lower variability compared to the standard was noted only in variety samples: UD0300577 – 333.0 g/m², repeatability coefficient – 0.98, and variation coefficient – 4.5%; UD0303753 is 329.0 g/m², repeatability is 0.97, and coefficient of variation is 7.1%. The vast majority of variety samples were characterized by higher variability in the level of productivity, this is due to the influence of adverse growing conditions that cause stress.

Potential impacts of the invasive Agrilus planipennis on various demographics in Winnipeg, Canada

The hardiness of Fraxinus spp. (ash) makes them a common municipal tree in adverse growing conditions. In Winnipeg, Manitoba, the impacts of Dutch elm disease resulted in increased reliance on Fraxinus, resulting in a monoculture of Fraxinus across the city. Emerald ash borer (EAB) (Agrilus planipennis) poses substantial risk to Winnipeg's urban forest, forecasted to result in the total loss of Fraxinus in Winnipeg. With a lens of distributional equity, this study examines associations between Fraxinus abundance and distribution and human population demographics at the Dissemination Area and Census Tract levels. There are positive correlations with Fraxinus abundance and distribution and median household income and proportion of the population that self-identifies as a visible minority. Population density was positively associated with Fraxinus abundance and distribution at the Census Tract level. These results underscore the importance of assessing the distribution of vulnerable trees as a component of green equity. Using information on urban forest management in response to EAB, potential issues and possible opportunities are discussed.

Glycogen, poly(3-hydroxybutyrate) and pigment accumulation in three Synechocystis strains when exposed to a stepwise increasing salt stress

The cyanobacterial genus Synechocystis is of particular interest to science and industry because of its efficient phototrophic metabolism, its accumulation of the polymer poly(3-hydroxybutyrate) (PHB) and its ability to withstand or adapt to adverse growing conditions. One such condition is the increased salinity that can be caused by recycled or brackish water used in cultivation. While overall reduced growth is expected in response to salt stress, other metabolic responses relevant to the efficiency of phototrophic production of biomass or PHB (or both) have been experimentally observed in three Synechocystis strains at stepwise increasing salt concentrations. In response to recent reports on metabolic strategies to increase stress tolerance of heterotrophic and phototrophic bacteria, we focused particularly on the stress-induced response of Synechocystis strains in terms of PHB, glycogen and photoactive pigment dynamics. Of the three strains studied, the strain Synechocystis cf. salina CCALA192 proved to be the most tolerant to salt stress. In addition, this strain showed the highest PHB accumulation. All the three strains accumulated more PHB with increasing salinity, to the point where their photosystems were strongly inhibited and they could no longer produce enough energy to synthesize more PHB.

EVALUATION OF PHYSIO-CHEMICAL CHARACTERISTICS AND ACRYLAMIDE CONTENT IN CRISP FRIED DOUGH WAFERS MADE FROM NIXTAMALIZED PEARL MILLET

Pearl millet (Pennisetum glaucum), a drought tolerant crop belonging to the family Poaceae is an important type of millet. It is a sturdy plant that can sustain adverse growing conditions. It is rich in vitamins, minerals, oil and phytochemicals. The phytates or phytic acid present acts as antinutritional factor which bind to the proteins and minerals thereby reducing the digestibility and bioavailability of the proteins, carbohydrates and minerals. In the following study, nixtamalization, a process widely used for the treatment of maize, is being used on the millet to lower the antinutritional properties of the millet. The grain was cooked at 95°C with varying lime concentration (0.5%, 1.0%, 1.5%, 2.0%) while keeping the cooking time (30 min) and steeping time (2 hours) constant. Product (crisp fried dough wafers) was made from the milled nixtamalized flour and different physio-chemical parameters and acrylamide content of the crisp fried dough wafer was analysed. The product made from flour treated with 1.5% lime showed the best results and overall acceptability. This research could be beneficial for increasing the utilization of pearl millet in different parts of the world especially in under developed countries as a health food. Also, the phytochemicals of pearl millet have various health benefits and this study can be used to increase their concentration and thereby promote the use of pearl millet for enrichment of various food products.

Evaluation of seed morphology, seedling genetic variation, and components for seed storage of Agave landraces of commercial interest

Abstract Sexual propagation of Agave plants is an incipient cultivation method, these plants withstand drought and adverse growing conditions; therefore, research on Agave’s diversity, seed processing, and storage could support its cultivation on marginal lands. The aim of this work was to evaluate seed morphology, germination, and seedling genetic diversity of six seed origins (species × provenance) of Agave plants collected in five provenances from Mexico. Seed longevity was evaluated in two seed origins after a 10-year storage period. Seed morphology and seedling genetic variation results demonstrated intra- and interspecific variation within Agave salmiana and with the other seed origins, respectively. After a 10-year storage period seed germination of two A. salmiana seed origins remained relatively stable, storage conditions, and seed variables of this work can serve as reference parameters for future analyses. To the best authors’ knowledge, this is the first report of Agave’s seed longevity evaluation after a 10-year storage period.

Selective influence of mannitol on culture of callus tissues of camelina sativa

Camelina sativa is an agricultural crop that provides valuable raw materials in different soil and climatic conditions with low production costs. Despite the prospects of camelina sativa, its sown areas in Ukraine remain insignificant. The main factor holding back the increase in the cultivation plan of this crop is the lack of highly productive, adapted to adverse growing conditions varieties. Involvement of biotechnological methods in the general scheme of selection process allows to increase efficiency of creation of genotypes of plants with desirable signs. The article presents the results of research on the effect of mannitol on the callus tissue of camelina sativa. The selective factor was added at various concentrations (2, 4, 6, 8, 10, 12 %) to the modified nutrient medium according to the Murashige-Skuga recipe. Morphogenic microcalls obtained from explants of camelina sativa Stepovy 1, Klondike, Peremoha and Euro 12 were planted on the nutrient substrate. At the end of subculturing, microcall viability, proliferation intensity and morphogenic characteristics of biomaterials were determined. It was found that, depending on the genotype, the concentration of mannitol at the level of 8–10 % is optimal for the selection of in vitro drought-resistant culture forms. High resistance to osmotic stress is characterized by biomaterials obtained from the varieties Stepovy 1 and Peremoha. Isolated camelina sativa cell lines with high resistance to osmotic stress have been identified, which can serve as donors of drought resistance genes in further breeding research.