Selective influence of mannitol on culture of callus tissues of camelina sativa

Abstract

Camelina sativa is an agricultural crop that provides valuable raw materials in different soil and climatic conditions with low production costs. Despite the prospects of camelina sativa, its sown areas in Ukraine remain insignificant. The main factor holding back the increase in the cultivation plan of this crop is the lack of highly productive, adapted to adverse growing conditions varieties. Involvement of biotechnological methods in the general scheme of selection process allows to increase efficiency of creation of genotypes of plants with desirable signs. The article presents the results of research on the effect of mannitol on the callus tissue of camelina sativa. The selective factor was added at various concentrations (2, 4, 6, 8, 10, 12 %) to the modified nutrient medium according to the Murashige-Skuga recipe. Morphogenic microcalls obtained from explants of camelina sativa Stepovy 1, Klondike, Peremoha and Euro 12 were planted on the nutrient substrate. At the end of subculturing, microcall viability, proliferation intensity and morphogenic characteristics of biomaterials were determined. It was found that, depending on the genotype, the concentration of mannitol at the level of 8–10 % is optimal for the selection of in vitro drought-resistant culture forms. High resistance to osmotic stress is characterized by biomaterials obtained from the varieties Stepovy 1 and Peremoha. Isolated camelina sativa cell lines with high resistance to osmotic stress have been identified, which can serve as donors of drought resistance genes in further breeding research.