Administration Of Honey Research Articles

Pediatric Esophageal Button Battery Protocol Reduces Time From Presentation to Removal.

Evaluate implementation of an institutional protocol to reduce the time to removal of esophageal button battery (BB) and increase use of mitigation strategies. We developed a protocol for esophageal BB management [Zakai's Protocol (ZP)]. All cases of esophageal BB impaction managed at a tertiary care center before and after implementation from 2011 to 2023 were reviewed. Time to BB removal, adherence to critical steps, and use of mitigation strategies (honey/sucralfate, acetic acid) were evaluated. Fifty-one patients (38 pre-ZP, 13 post-ZP) were included. Median age was 2.3 years (IQR 1.3-3.4). After implementation, the time from arrival at the institution to arrival in the operating room (OR) reduced by 4.2 h [4.6 h (IQR 3.9-6.5) to 0.4 h (IQR 0.3-0.6), p < 0.001] and there was improvement in all management steps. The number of referrals direct to otolaryngology increased from 51% to 92%, arrival notification increased from 86% to 100%, avoidance of second x-ray increased from 63% to 100%, and direct transfer to OR increased from 92% to 100%. Adherence to mitigation strategies such as preoperative administration of honey or sucralfate increased from 0% to 38%, intraoperative use of acetic acid from 3% to 77%, and nasogastric tube insertion from 53% to 92%. Implementation of ZP substantially reduced the time to BB removal and the use of mitigation strategies in our tertiary care institution. Additional strategies focused on prevention of BB ingestion, and shortening the transfer time to the tertiary care hospital are required to prevent erosive complications. Level 3 Case-series Laryngoscope, 2024.

The Effectiveness of Honey Administration on Reducing the Frequency of Diarrhea in Children with Acute Gastroenteritis in the Carnation Room of Rsud Waled Cirebon District: Case Study

One of the causes of death in children is diarrhea. Diarrhea is an increased frequency of bowel movements and occurs more than 3x and with a soft and watery consistency due to the water content in the stool &gt;200 ml / 24 hours which lasts &lt; than 14 days. The purpose of this study is to determine the effectiveness of giving honey to reduce the frequency of diarrhea in children. Method: The method uses a qualitative approach with case studies as the main method. Data collected through interviews, documentation, data analysis to the provision of intervention and evaluation. The research was conducted in the Carnation Room of Waled Hospital, Cirebon Regency in February 2024. The sample in this study was children treated with a diagnosis of acute gastroenteritis that focused on nursing problems, namely diarrhea. Honey intervention 3 times / day is given as much as 5 ml / 6 hours to children. Results: based on research that has been done researchers found that giving honey can reduce the frequency of diarrhea in children because of the content in honey

Hepatic and renal effects of oral stingless bee honey in a streptozotocin-induced diabetic rat model.

Diabetes is known damage the liver and kidney, leading to hepatic dysfunction and kidney failure. Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications. However, the effect of stingless bee honey (SBH) administration in relieving liver and kidney damage in diabetes has not been well-studied. To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced (STZ; 55 mg/kg) diabetic Sprague Dawley rats. The rats were grouped as follows (n = 6 per group): non-diabetic (ND), untreated diabetic (UNT), metformin-treated (MET), and SBH+metformin-treated (SBME) groups. After successful diabetic induction, ND and UNT rats were given normal saline, whereas the treatment groups received SBH (2.0 g/kg and/or metformin (250 mg/kg) for 12 d. Serum biochemical parameters and histological changes using hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining were evaluated. On H&E and PAS staining, the ND group showed normal architecture and cellularity of Bowman's capsule and tubules, whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane. The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining, but the two were similar on PAS staining. Likewise, the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining, but it was comparable to the SBH and ND groups on PAS staining. UNT diabetic rats had tubular hydropic tubules, which were smaller than other groups. Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group. Conversely, the UNT group had high glucose levels, which subsequently increased MDA levels, ultimately leading to liver damage. SBH treatment reduced this damage, as evidenced by having the lowest fasting glucose, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase levels compared to other groups, although the levels of liver enzymes were not statistically significant. The cellularity of the Bowman's capsule, as well as histological alteration of kidney tubules, glomerular membranes, and liver tissues in diabetic rats after oral SBH resembled those of ND rats. Therefore, SBH exhibited a protective hepatorenal effect in a diabetic rat model.

Pumpkin seeds (Cucurbita pepo subsp. ovifera) decoction promotes Trichinella spiralis expulsion during intestinal phase via “Weep and Sweep” mechanism

Trichinosis is a zoonotic disease of communal health concern as it instigated human outbreaks in several countries. Besides, the development of resistance, traditional therapy has numerous antagonistic effects. Thereby, finding efficient natural alternatives is required. In comparison to albendazole, this study evaluated the impact of pumpkin decoction on Trichinella spiralis in experimentally infected mice. The anthelmintic action of pumpkin decoction (500 mg/kg) was determined using T. spiralis infected mice in enteric phase for 5 days. Pumpkin decoction anthelmintic activity fortified by mixing with honey (1:1). Pumpkin decoction and Pumpkin decoction-honey mixture were evaluated by comprising with reference drug, albendazole (50 mg/kg). The T. spiralis adult count was significantly lower in all treated groups, with the pumpkin decoction-honey mixture showing the largest reduction (83.2%) when compared to the infected group (P ≤ 0.001). The intestinal histological changes and the level of COX-2 expression in the intestinal tissue were both significantly reduced in the same group. The pumpkin decoction improved the immune response, as evidenced by a significant decrease in nitric oxide (NO) and tumor necrosis factor (TNF-α) and a significant increase in the expression of the transforming growth factor (TGF-1β) and interleukin-17 (IL-17). The pumpkin decoction's anthelmintic action was facilitated by the TGF-1β and IL-17-driven Weep and Sweep mechanism. Both administration of pumpkin decoction beside honey showed the best treatment group that resulted in high infection reduction besides amelioration of biochemical markers and restoration of histological to normal state. In conclusion, pumpkin decoction is highly effective against T. spiralis which could be a promising alternative herbal drug and the pumpkin decoction effect was higher in the case of combination with honey.

The impact of Apis dorsata forest honey administration on follicle-stimulating hormone and luteinizing hormone levels in rats (Rattus norvegicus) forced swim test as a chronic physical animal model.

Chronic physical stress has many effects on the nervous system and can cause structural changes in different parts of the brain and hemomodulatory, including hormonal. Current pharmacotherapeutic treatments have limited efficacy and are associated with many deleterious side effects. The aim of this research is to determine how Apis dorsata forest honey administration affects follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels in rats who are subjected to forced swim tests as a model of chronic physical stress placed in a container filled with water from which it cannot escape. This was an experimental laboratory study with 32 rats divided into four treatment groups: control (C), Treatment 1 (T1) with a forced swim test + honey (2 g/rat/day), Treatment 2 (T2) with a forced swim test + honey (4 g/rat/day), and Treatment 3 (T3) with a forced swim test + honey (6 g/rat/day). All treatments were administered for 14 days. Then, blood was taken for FSH and LH serum tests, and a one-way ANOVA and Duncan test were used to statistically test the data analysis. The results of this study indicate that the administration of forest honey had no significant effect (p > 0.05) on the FSH parameter, but there was a significant decrease in LH levels in the T2 and T3 groups (p < 0.05). It can be concluded that giving forest honey to rats who were subjected to a 14-day forced swim test had no effect on FSH and LH levels. In rats given a forced swim test as a model of chronic stress, administration at doses of 4 and 6 g/rat/day reduced LH serum levels. Thus, giving forest honey could maintain reproductive health in rat that experience chronic stress.

Effects of honey administration on transforming growth factor beta-1 levels during orthodontic tooth movement in young and older rats

Effects of honey administration on transforming growth factor beta-1 levels during orthodontic tooth movement in young and older rats

Calliandra honey and nutritional deficiency influence to spermatozoa motility and viability in Rattus norvegiccus

Nutritional deficiency or malnutrition is one of the severe health problems that causes changes in body weight, body composition, and physical function. The potential of calliandra honey to spermatozoa motility and viability, in the male white rat (Rattus norvegiccus) undergoing nutritional deficiency will be investigated in this research. 24 male white rats with body weights 250-300 grams were used and divided into four groups with each group containing six white rats. The first group was the negative control (C-) group, C+ was the group with fasting condition but without honey administration, T1 and T2 were the fasting treatment in ten days with 30% and 50% honey, respectively. The result was significance differences between C-group with C+ or T1 and T2 (p<0.05). The T1 and T2 can increase the motility and viability of spermatozoa and the difference significant with C+ (p<0.05), but it cannot restore the motility and viability of spermatozoa to normal conditions. It can be concluded that the administration of Calliandra honey in the T1 and T2 with concentrations of 30% and 50% can already improve the quality of spermatozoa which can be seen from the motility and viability of spermatozoa.

The Effect of Administering Forest Honey to Rats Exposed to Physical Stress on Corticosteroid Levels, Folliculogenesis and the Number of Corpus Luteum

Livestock that has stress releases glucocorticoids in response to it, and it causes inhibition of the hypothalamus-pituitary-gonadal axis (HPG) signaling pathway so that it reduces reproductive efficiency. Forest honey can reduce corticosteroid levels as a stress response from physical stress induction which is expected to increase reproductive efficiency including folliculogenesis and the formation of the corpus luteum. This study aims to determine the effect of forest honey on rats (Rattus novergicus) exposed to physical stress on corticosteroid levels, folliculogenesis, and the number of corpus luteum. This study is an experimental laboratory one using 32 rats which were divided into 4 treatment groups; control positive (C) treated with physical stress, treatment 1 (T1) treated with physical stress + honey 2 g/rat/day PO, treatment 2 (T2) treated with physical stress + honey 4 g/rat/day PO and treatment 3 (T3) treated with physical stress + honey 6 g/rat/day PO. All treatments were carried out for 14 days. The results showed that T1 had the lowest corticosteroid level compared to all treatment groups and the corticosteroid level of this group was significantly different (p &lt;0.05) compared to that of C and T3. The folliculogenesis profile showed that the number of primary secondary, tertiary, and Graafian follicles of group T1 was significantly different (p&lt;0.05) compared to that of C, T2, and T3. In terms of the number of corpus luteum, it showed that T1 had the highest number of corpus luteum, and the number of corpus luteum in this group was significantly different (p&lt;0.05) from that of C, T2, and T3. It can be concluded that the administration of forest honey at a dose of 2g/rat/day could reduce corticosteroid levels, improve the folliculogenesis profile, and increase the number of corpus luteum in rats exposed to physical stress. The use of forest honey could reduce corticosteroid levels as a stress response from physical stress induction which was expected to increase reproductive efficiency.

The use of honey in button battery ingestions: a systematic review.

Button battery (BB) ingestions may cause severe and possibly fatal complications, especially if the battery is located in the esophagus. The application of oral honey has recently been proposed by the National Capital Poison Center in the USA and in an ESPGHAN position paper in Europe, but clinical trials and experimental studies are limited. The goal of this systematic review was to analyze the evidence for this approach. A systematic review of clinical trials and experimental studies on the oral application of honey after BB ingestion in children was performed. Inclusion criteria according to the PICO format were patient age 0-18 years, ingestion of BB, oral administration of honey or other substances, all in vivo and in vitro studies, as well as reported complication rate, esophageal injury, and mortality. A manual search in the databases MEDLINE, Web of Science and Cochrane was performed to identify relevant search terms to form the following queries and to construct the extensive search. Furthermore, the search was extended by using snowballing on the reports reference lists. The review is registered at Research Registry. The identifying number is reviewregistry1581. We found four publications that investigated the effects of honey after button battery ingestion. Three of these presented experimental in vitro and in vivo results and one reported a clinical retrospective study of 8 patients. Follow up studies are required to further elucidate the effectiveness of the treatment with honey. The time intervals in which the use of honey is effective is not clear. Furthermore, a physiological model is needed for in vitro testing, preferably mimicking peristalsis and dynamic flow of the applied substances. However, since it is easy to apply and of minimal risk in patients over one year of age, honey should be considered a possible treatment option during the interval between presentation and endoscopic removal of the retained BB. https://www.researchregistry.com/browse-the-registry#registryofsystematicreviewsmeta-analyses/registryofsystematicreviewsmeta-analysesdetails/643e9df96750410027ee11b0/, identifier: reviewregistry1581.

The Effect of Natural Honey Administration on Some Sperm Function Parameters in Male Infertility Patients

The study was conducted in the infertility treatment center located in Al- Kut city of Iraq, the sample were taken from 43 individual have a weakness in firtility , aged between 20-40 years and have no diseases affect the study. Seminal fluid samples were collected by masturbation after 3-5 day abstention in sterile container and send to laboratory for measuring the concentration of fructose and the percentage of count , motility and morphology .Venous blood was drawn to measure the level of hormones LH, FSH and Testosterone .50 g of natural honey was given to patients to be eaten daily with breakfast for 30 days. After that seminal fluid and blood samples were collecting to measure the previous seminal parameters.The study found that there was a significant increase in fructose level , count , motility and the morphology. As well as there is an increase in the level of hormone testosterone, LH, FSH

Consumption of honey ameliorates lipopolysaccharide-induced intestinal barrier dysfunction via upregulation of tight junction proteins.

The leaky gut barrier is an important factor leading to various inflammatory gastrointestinal disorders. The nutritional value of honey and variety of its health benefits have long been recognized. This study was undertaken to assess the role of Indian mustard honey in preventing lipopolysaccharide (LPS)-induced intestinal barrier dysfunction using a combination of in vitro and in vivo experimental model systems. LPS was used to induce intestinal barrier damage in a trans-well model of Caco-2 cells (1µg/ml) and in Swiss albino mice (5mg/kg body weight). Gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) were used to analyse sugar and phenolic components in honey samples. The Caco-2 cell monolayer integrity was evaluated by transepithelial electrical resistance (TEER) and paracellular permeability assays. The histopathology of intestinal tissue was analysed by haematoxylin and eosin dual staining. The quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to quantify the transcription of genes. The protein expression was analysed by immunofluorescence, western blot and ELISA-based techniques. The in vitro data showed that honey prevented LPS-induced intestinal barrier dysfunction dose dependently as was measured by TEER and paracellular flux of FITC-dextran dye. Further, the in vivo data showed a prophylactic effect of orally administered honey as it prevented the loss of intestinal barrier integrity and villus structure. The cellular localization and expression of tight junction (TJ) proteins were upregulated along with downregulation of pro-inflammatory cytokines in response to the administration of honey with LPS. The findings of this study suggest a propitious role of honey in the maintenance of TJ protein integrity, thereby preventing LPS-induced intestinal barrier disintegration.

Effect of Honey Administration on The Histopathology of The Duodenum of Wistar Rats as a Inhibition of The Toxic Effects of Borax (Sodium tetraborate)

Background: Borax can result in oxidative stress and cause the onset of gastrointestinal ulcerations that will dampen the duodenal villi to become shorter and can even disappear. Cell damage due to oxidative stress can be prevented by administering antioxidants. Antioxidants will inhibit the onset of chain reactions in the formation of free radicals by complementing the existing electron deficiency. Honey is one of the natural ingredients that is rich in antioxidants and part of thibbun Nabawi as one of the inhibitors of the toxic effects of borax. Objective : The purpose of this study is to to analyze the effect of honey administration on the histopathological description of submucosa and epithelial mucosa of duodenal wistar rats as an inhibitor of the toxic effects of borax (Sodium tetraborate). Methods: This study used the true experimental method with Post Test Only Control Group Design using 25 mice which will be divided into five groups, namely K (negative control), P (positive control), M1 (borax and honey dose 1), M2 (borax and honey dose 2), M3 (borax and honey dose 3). This study was conducted for 22 days then. Took the duodenal organ on all samples and then made histological preparations with HE staining. Observations were made with an Olympus microscope to see submucosal edema and damage to the epithelium of the duodenal mucosa. Results: The results of the study found that borax 26 mg / head / day had an influence on the histopathological picture of duodenal borax. In addition, the administration of honey dose 75 mg / Kg BB provides a toxic inhibitory effect of borax in the duodenum best among other treatment groups. However, statistically there was no effect of honey administration on the histopathological picture of the duodenum of wistar rats as an inhibitor of the toxic effect of borax (Sodium Tetraborate) with p˃0.05. Conclusion: Statistically it can be concluded that there is no effect of the administration of honey on the histopathology picture of the duodenum of wistar rats as an inhibitor of the toxic effects of borax (Sodium tetraborate).

The Effectiveness Of The Administration Of Beet Juice And Honey Against Increased Hemoglobin Levels In Nursing Mothers

WHO in 2020, said some 295,000 women worldwide lost their lives during and after pregnancy and childbirth. Africa, sub-Saharan and South Asia account for around 86% of all maternal deaths worldwide. MMR in Indonesia in 2020 is 230 per 100,000 live births. Causes of maternal death are bleeding, hypertension, infection, prolonged labour, abortion, CED and anemia (Hb &lt;11gr). The prevalence of anemia in pregnant and lactating women in Indonesia is 48.9%. The impact of anemia on pregnant women will occur during pregnancy, childbirth and the postpartum period. Knowing the effectiveness of giving beetroot juice and honey to increase hemoglobin levels in nursing mothers. Quasy Experiment with Pretest Posttest With Control Group Design The sample in this study were lactating mothers with anemia who were registered at the KIA PMB Samsa Qomariyah register in May-October 2022 as many as 40 people, the sampling technique was total sampling. The average HB level in the intervention group was 10.175 gr/dl pretest and 10.865 gr/dl posttest. The average HB level in the control group was 11.250 gr/dl pretest and 11.275 gr/dl posttest. Beetroot juice and honey are effective in increasing hemoglobin levels in lactating mothers with a p.value of 0.000 (p &lt;0.005). Beetroot juice and honey are effective in increasing hemoglobin levels in nursing mothers. It is expected that breastfeeding mothers consume vegetables and fruit that contain lots of iron and vitamin C to increase HB levels and prevent anemia.

Effect of The Combination of Kefa Forest Honey and Euphorbia hirta as a Curative agent of Vibrio alginolyticus in the Hybrid Grouper Epinephelus fuscoguttatus

Kefa forest honey and Euphorbia hirta have an antibacterial effect against pathogenic bacteria in fish. This work investigated the combination of honey and E. hirta against Vibrio alginolitycus in the hybrid grouper Epinephelus fuscoguttatus. The active ingredients and antibacterial activity of Kefa forest honey and E. hirta were tested in vitro at different concentrations using the disk method. The experimental animals were injected with V. alginolitycus suspension at a concentration of 106 cells ml−1/fish. After the fish showed symptoms of infection, the treatment was carried out for 5 consecutive days by immersion in the mixture of 25% Kefa honey and E. hirta at different concentrations of honey and E. hirta combinations. After treatment, the fish were monitored for 7 days. During the study, observations on hematology and clinical symptoms were made. The results showed that the combination of Kefa honey and E. hirta had antibacterial activity against V. alginolitycus in vitro and in vivo can increase the hematological profile of grouper and alleviate clinical symptoms due to V. alginolitycus infection. The results showed that administration of a combination Kefa forest honey and E. hirta was effective as a cure for V. alginolyticus infection in grouper.

Neuroprotective effects of Apis dorsata forest honey on the neurons count in cerebrum and cerebellum of mice (Mus musculus) exposed to monosodium glutamate

Introduction: Monosodium glutamate or MSG can have an effect on increasing the production of reactive oxygen species (ROS) in the brain. Apis dorsata forest honey is known to have higher antioxidant activity than Apis mellifera and Apis cerana honey. This study aims to determine the preventive effect of Apis dorsata honey on the number of pyramidal cerebrum neurons and purkinje cerebellum neurons of mice (Mus musculus) exposed to monosodium glutamate (MSG). Material and Methods: This study used 25 mice which were divided into 5 groups. In the K- group only aquadest was given. The K+ group was given 4mg/gBB of MSG. Groups P1, P2, and P3 given Apis dorsata honey at a dose of 53.82mg/20gBW, 107.64mg/20gBW, and 161.46mg/20gBW. All treatments were carried out orally for 52 days. Results: The results showed that the average number of cerebral pyramidal neurons in the K-, K+, P1, P2, and P3 groups was 12.24±0.607; 7.24±2.875; 12.48±1.513; 15.72±0.944; and 19.28±2.827. The mean number of purkinje cerebellar neurons was 4.8±1.456; 2.08±0.807; 3.08±1.035; 3.56±0.434; and 4.68±1.390. Discussion: The average number of pyramidal neurons and purkinje neurons in the group given preventive dose of Apis dorsata honey and exposed to MSG was higher and there is a significant difference compared to the group that was only exposed to MSG. It can be concluded that the administration of Apis dorsata honey can maintain the number of pyramidal cerebrum neurons and purkinje cerebellar neurons in mice exposed to MSG.

Modulation of vulvovaginal atrophy (VVA) by Gelam honey in bilateral oophorectomized rats.

Vulvovaginal atrophy (VVA) is a common condition in post-menopausal women. Symptoms of VVA include dyspareunia, vaginal dryness, vaginal and/or vulvar itching, burning and soreness, dysuria and vaginal bleeding accompanying sexual activity. These symptoms are physiological responses to hypoestrogenicity, inducing atrophy of the vagina epithelia and sudden reduction in mucous production. Prevailing therapy for VVA is hormone replacement therapy (HRT), notably estrogen, progesterone or a combination of the two. However, using HRT is associated with an increased incidence of breast and endometrial cancer, venous thromboembolism in the lungs and legs, stroke and cardiovascular complications. This study evaluated Malaysian Gelam honey as a nutraceutical alternative to estrogen HRT (ERT) in alleviating VVA. A total of 24 female 8-weekold Sprague Dawley rats underwent bilateral oophorectomy. A minimum of 14 days elapsed from the time of surgery and administration of the first dose of Gelam honey to allow the female hormones to subside to a stable baseline and complete recovery from surgery. Vaginal tissues were harvested following a 2-week administration of Gelam honey, the harvested vagina tissue underwent immunohistochemistry (IHC) analysis for protein localization and qPCR for mRNA expression analysis. Results indicated that Gelam honey administration had increased the localization of Aqp1, Aqp5, CFTR and Muc1 proteins in vaginal tissue compared to the menopause group. The effect of Gelam honey on the protein expressions is summarized as Aqp1>CFTR>Aqp5>Muc1. Gene expression analysis reveals Gelam honey had no effect on Aqp1 and CFTR genes. Gelam honey had up-regulated Aqp5 gene expression. However, its expression was lower than in the ERT+Ovx group. Additionally, Gelam honey up-regulated Muc1 in the vagina, with an expression level higher than those observed either in the ERT+Ovx or SC groups. Gelam honey exhibits a weak estrogenic effect on the genes and proteins responsible for regulating water in the vaginal tissue (Aqp1, Aqp5 and CFTR). In contrast, Gelam honey exhibits a strong estrogenic ability in influencing gene and protein expression for the sialic acid Muc1. Muc1 is associated with mucous production at the vaginal epithelial layer. In conclusion, the protein and gene expression changes in the vagina by Gelam honey had reduced the occurrence of vaginal atrophy in surgically-induced menopause models.

The Effect of Administering Apis dorsata Forest Honey on Liver Histopathological Changes of Rats (Rattus norvegicus) Exposed to Physical Stress

The objective of this study was to determine the effect of Apis dorsata forest honey on the liver histopathological changes of white rats (Rattus norvegicus) exposed to physical stress. This study used 24 rats which were divided into 4 groups. The positive control group (C+) was only given a stressor and distilled water orally, the treatment group 1 (T1) was given a stressor + honey 2g/rat/day, T2 was given a stressor + honey 4g/rat/day, and T3 was given a stressor + honey 6g/rat/day. All treatments were carried out within 14 days. The results showed that there was a significant difference (p&lt;0.05) between the C+ control group (3.44a ± 0.622; 3.52a ± 0.109; 1.76a ± 0.384) and the treatment group1 /T1 (1.13b ± 0.389; 2, 48c ± 0.438; 0.84b ± 0.260) in terms of reduced level of liver damage, however, there was no significant difference (p&gt; 0.05) between the C+ control group (3.44a ± 0.622 ; 3 .52a ± 0.109; 1.76a ± 0.384) and treatment group2 / T2 (3.72a ± 0.268; 3.64ab ± 0.260; 1.90a ± 0.400) and T3 (3.84a ± 0.167; 3.88a ± 0.178; 2 .48a ± 0.840). It can be concluded that the administration of Apis dorsata honey to rats exposed to physical stress within 14 days can reduce the level of liver damage with an effective dose of 2g/rat/day. However, excessive consumption of honey causes adverse effects on the liver as seen in the T2 and T3 treatment groups with doses of honey 4g/rat/day and 6g/rat/day which can increase the risk of liver damage.

COMPARATIVE PERFORMANCE OF LAYER STRAIN CHICKS REARED IN SEMI ARID REGION AND FED HONEY AS AN ADDITIVE

Two experiments were conducted to evaluate the performance of two strains of layer chick reared in the semi-arid region and feeding of honey in place of an additive. In the first experiment 90 day old chicks of each of two egg layer strains, made up of one brown (Lohman Brown classic), one black (Nera Black) were used in completely randomized with each strain serving as a treatment. Each treatment was replicated thrice with 30 chicks per replicate. In the second experiment, 84 seven weeks old chicks from each of the strains were used in a 2 x 2 factorial arrangement in a completely randomized design with the two strains (Lohmann brown classic and Nera black) and administration of honey (with and without) as the factors. These made up four treatments which were replicated thrice with 14 chicks per replicate. In both experiments, feed intake and body weight were measured and weight gain and feed conversion ratio were determined. Mortality was also recorded. The results of the study showed that performance parameters of two strains measured were statistically similar except for mortality. Nera black strain had a higher mortality. Feeding honey did not affect (P&gt;0.05) performance indicators measured except for mortality also. Mortality was higher (P&lt;0.05) for chicks that were not fed with honey. According to these results, Nera black strain may require more care during chick phase and honey has beneficial effect on chick performance.

The Role of Prebiotics for Diversity of Intestinal Microorganisms of Tilapia (Orechromis niloticus)

Prebiotics are undigested food ingredients that can be utilized by intestinal microorganisms. The provision of prebiotics will improve growth performance, inhibit the growth of pathogens, and increase fish immunity. Several previous aquaculture studies have used single prebiotics as prebiotics, such as FOS, GOS, and inulin. We need a material that is multi prebiotics which is expected to give better results. Honey is a material that includes multi prebiotics. The purpose of this study was to evaluate the administration of prebiotic honey with different doses (0%, 05%, and 1% doses) through feeding on the diversity of microbiota in the digestive tract of tilapia (Oreochromis niloticus). The method used in this study was to take samples of tilapia intestines, then analyzed them using Next Generation Sequencing (NGS). The results showed that the most unique OTU were in the treatment of prebiotic honey with a dose of 1%. The treatment of prebiotic honey in the feed gave a higher OTU compared to the control. This is because the oligosaccharide content of honey can be utilized by the gut microbiota. The high unique OTUs in the treatment of adding honey, both 0.5% and 1%, presumably because these unique OTUs can grow well in that environment so that there is an equilibrium between species that together can utilize nutrients from honey oligosaccharides. so that all components of the microbiota can grow optimally. Prebiotic honey given to tilapia also triggers the emergence of the genus Lactobacillus, Cetobacterium, and Clostridium sensu stricto which are probiotic bacteria in the fisheries sector.

Pemberian Jintan Hitam (Nigella Sativa L) dan Madu (Apis Mellifera) Sebagai Suplemen Zat Besi dalam meningkatkan Kadar Hemoglobin Ibu Hamil

Introduction: Iron deficiency anemia is one of the most common disorders during pregnancy. Pregnant women generally experience a decrease in iron so only a small amount of iron in the fetus is needed for normal iron metabolism. During pregnancy, the indication of anemia is if the HB concentration is less than 11 g% in the first and third trimesters and the HB level is less than 10.5 g% in the second trimester Objectives: The purpose of this study was to determine the effect of giving nigella sativa oil and honey on hemoglobin levels of pregnant women at PMB Rosminta Siregar Bojong Gede in 2022 Method: This study used a quasi-experimental design using one group pre-test and post-test methods. The number of samples is 30 pregnant women. The research instrument used observation sheets and statistical tests using the dependent t-test. Result: The results showed that there was a significant difference in hemoglobin levels of pregnant women before and after giving Nigella Sativa and Honey with a 2-tailed sign of 0.000. It can be seen that the 2-tailed sign is 0.000 &lt; (0.05). Conclusion: The study concluded that there were differences in the administration of nigella sativa oil and honey on the hemoglobin levels of pregnant women at PMB Rosminta Siregar Bojong Gede in 2022.