Neuroprotective effects of Apis dorsata forest honey on the neurons count in cerebrum and cerebellum of mice (Mus musculus) exposed to monosodium glutamate

Abstract

Introduction: Monosodium glutamate or MSG can have an effect on increasing the production of reactive oxygen species (ROS) in the brain. Apis dorsata forest honey is known to have higher antioxidant activity than Apis mellifera and Apis cerana honey. This study aims to determine the preventive effect of Apis dorsata honey on the number of pyramidal cerebrum neurons and purkinje cerebellum neurons of mice (Mus musculus) exposed to monosodium glutamate (MSG). Material and Methods: This study used 25 mice which were divided into 5 groups. In the K- group only aquadest was given. The K+ group was given 4mg/gBB of MSG. Groups P1, P2, and P3 given Apis dorsata honey at a dose of 53.82mg/20gBW, 107.64mg/20gBW, and 161.46mg/20gBW. All treatments were carried out orally for 52 days. Results: The results showed that the average number of cerebral pyramidal neurons in the K-, K+, P1, P2, and P3 groups was 12.24±0.607; 7.24±2.875; 12.48±1.513; 15.72±0.944; and 19.28±2.827. The mean number of purkinje cerebellar neurons was 4.8±1.456; 2.08±0.807; 3.08±1.035; 3.56±0.434; and 4.68±1.390. Discussion: The average number of pyramidal neurons and purkinje neurons in the group given preventive dose of Apis dorsata honey and exposed to MSG was higher and there is a significant difference compared to the group that was only exposed to MSG. It can be concluded that the administration of Apis dorsata honey can maintain the number of pyramidal cerebrum neurons and purkinje cerebellar neurons in mice exposed to MSG.