Administration Of Honey Research Articles

PENGARUH PEMBERIAN JUS KACANG HIJAU DAN MADU TERHADAP KADAR HEMOGLOBIN PADA IBU HAMIL

Anemia is a problem that often accompanies pregnancy. The most common anemia during pregnancy is nutritional anemia caused by iron deficiency. Pregnant women are considered to have anemia if their hemoglobin (Hb) level <11 gr / dL. Anemia can worsen the birth process. Anemia can be overcome with pharmacological and non-pharmacological treatments, one of the non-pharmacological treatments for the prevention of anemia in pregnant women is to consume green bean juice and honey. Because green beans and honey contain iron and vitamins that can increase hemoglobin levels. This study aims to determine the effect of giving green bean juice and honey on hemoglobin levels in pregnant women. The research method used was quasi-experimental (Quasi Experiment) with one group pre and post test design. The study population was all pregnant women who performed ANC at the Maiharti West Range Midwife clinic. The sampling technique used purposive sampling with the results of 19 pregnant women. The results showed that from using the Paired Sample T-Test analysis of giving green bean juice and honey to increase hemoglobin levels in pregnant women where the p 0,00 <α = 0,05. It can be concluded that the administration of green bean juice and honey is effective in increasing hemoglobin levels

POTENSI MADU PADA PENINGKATAN JUMLAH SEL SPERMATOGENIK TIKUS (Rattus norvegicus) YANG KEKURANGAN NUTRISI

This study aimed to determine honey's potential in increasing the number of spermatogenic cells (spermatogonium, spermatocyte and spermatid) of rats (Rattus norvegicus) with nutritional deficiencies. Twenty four male rats (Rattus norvegicus) were randomly divided into four groups. Negative control (K-) mice was not fasted nor given honey. Positive control (K+) mice was fasted for 5 days without the administration of honey. The treatment group rats were fasted for 5 days followed by administration of honey 30% (P1) and 50% (P2) in drinking water for 10 days. On the 76th day all rats were sacrificed and their testes were collected for histological slides using Hematoxylin-Eosin staining. The results showed that fasting treatment for five days (group K +) caused a decrease (p <0.05) in the number of spermatogonia cells, spermatocytes and spermatids compared to those of the normal mice (group K–). Administration of 50% honey (group P2) for 10 days caused an increase (p <0.05) in the number of spermatogonia cells, spermatocytes and spermatids compared to the K + group mice. In P2 group, the number of spermatogonia cells was lower (p <0.05), while the number of spermatocytes and spermatids was not significantly different than in the K– group mice. It could be concluded that the administration of honey was able to regenerate the testicular tissue of rats with nutritional deficiency by increasing the number of spermatogenic cells in the seminiferous tubules.

The Effect of Pineapple Juice and Honey on the Acceleration of Perineal Wound Healing in Post-Partum Mothers in the Work Area of Dr. M.M. Dunda Limboto Hospital

The purpose of this research is to determine the effect of pineapple juice and honey on the acceleration of perineal wound healing in puerperal mothers, with a quasi-experimental design. The research design chosen was Post Test Only Control Group Design using purposive sampling technique with a sample of 36 respondents. The experimental group 18 which was given pineapple juice and honey for 7 days was 150 ml 2x1 then measured by post-test after the treatment while the control group 18 respondents who were not given treatment. The results showed that administration of pineapple juice and honey had a significant effect on the acceleration of perineal wound healing in postpartum mothers using the Whitney mann test to obtain a mean of 24.50-12.50 mean value of 12 ρ value 0,000 <α 0.05.

Protective Effect of Thyme Honey against Valproic Acid Hepatotoxicity in Wistar Rats.

Introduction Valproic acid is a medication most commonly used in the treatment of emotional and neurological depression, psychological imbalances, epilepsy, and bipolar disorder. Dark honey, like thyme honey, contains more antioxidant compounds than other samples. The purpose of this study was to evaluate the effect of thyme honey on the potential hepatic effects of valproic acid. Methods In this study, 48 male rats were randomly divided into 8 groups (n = 6): G1 (control): healthy rats (normal saline 0.9%), G2: thyme honey (1 g/kg), G3: thyme honey (2 g/kg dose), G4: thyme honey (3 g/kg dose), G5: VPA (500 mg/kg), G6: VPA (500 mg/kg) and thyme honey (1 g/kg), G7: VPA (500 mg/kg) and thyme honey (2 g/kg dose), and G8: VPA (500 mg/kg) and thyme honey (3 g/kg dose). Groups G1 to G5 received the drug for 28 days. On day 14, administration of thyme honey for G6 to G8 groups was carried out using gavage until day 28. VPA was administered one hour after honey. To carry out the biochemical evaluation, blood samples were collected from all the groups and their serums were used for MDA, TAC, and liver enzymes (AST, ALT, and GGT). Tissue samples of each rat were also removed for histological studies with hematoxylin-eosin and Masson's trichrome staining. Results The use of thyme honey significantly improved the histopathological parameters of the liver tissue, including hypertrophic degeneration and nucleus alteration, expansion of sinusoids, fibrosis and hepatic necrosis, and inflammation as well as hypertrophy of Kupffer cells. In the groups receiving VPA, the rate of lipid peroxidation increased, which indicates the destruction of the liver cell membrane due to drug consumption. TAC levels also increased following increase in thyme honey dosage (p ≤ 0.05). The results of liver enzyme analysis showed a decrease in AST and ALT levels in the G6 group and a decrease in GGT level in the G8 group (p ≤ 0.05). Conclusion Based on the results of this study, it seems that high percentage of antioxidants in thyme honey enabled it to improve hepatic complications and reduce the rate of hepatocellular destruction.

Gene expression and immune response of pacific white shrimp given Bacillus NP5 probiotic and honey prebiotic and Vibrio parahaemolyticus infection

ABSTRACT The present study aimed to evaluate the growth performance, immune response, and immunity-related genes in white shrimp given the Bacillus sp. (NP5) probiotic and honey prebiotic and V. parahaemolyticus infection. This study consisted of five treatments: NP5, honey, synbiotic (honey and NP5), positive control (PC), and negative control (NC), respectively. The results of this study demonstrated that the administration of the NP5, honey, and synbiotic could improve the growth performance, immune response, and expression of immunity-related genes with the best results in the synbiotic treatment, also better than those in treatment PC, both after the treatments and after the challenge test. The survival rate post-challenge test revealed that the results for treatment with NP5, honey, and synbiotic were higher (73.3–86.7%) than that of treatment PC (50.0%). In conclusion, the administration of NP5, honey, and synbiotic could improve the growth performance and immunity of white shrimp against V. parahaemolyticus infection.

Growth performance of tambaqui (Colossoma macropomum) supplemented with honey prebiotic in stagnant peat ponds

This study aims to evaluate the effectiveness of giving honey as a prebiotic in increasing the growth performance of freshwater pomfret (Colossoma macropomum). In this study, honey was mixed into the feed with the method coated at doses of 0.0%, 0.3%, 0.6%, and 0.9%. Fish with an initial body weight of 3-5 g are reared in three ponds with a total of 12 hapa nets measuring 1x1x1 (m3) (40 fish / hapa nets), that is, 4 hapa nets are placed in each pond. Thus, each treatment replication could be represented in each pond. Fish were fed treated with three replications for 60 days. The best growth performance during 60 days of maintenance indicated that honey supplementation with a dose of 0.6% was significantly different from other treatments in terms of the amount of feed intake 1195±0.01 g, FCR 1.12±0.00, feed efficiency 90.34±0.00 %, the daily growth rate was 3.88±0.02 %, and the body weight gain rate was 778.53±0.01 %. It can be concluded that the administration of honey with a dose of 0.6% is effective in improving the feed conversion ratio, increasing feed efficiency, daily growth rate, body weight gain, and reducing the amount of feed consumption of freshwater pomfret in inundated peat fish ponds.

Honey reverses disease progression, has anti-nociceptive and anti-inflammatory effects in a rat model of knee osteoarthritis induced by monosodium iodoacetate

<h2>Summary</h2><h3>Background</h3> Clinically, osteoarthritis manifests as joint pain with concomitant loss of joint function, which may ultimately result in a substantially reduced quality of life for the patient. Although, a lot is known about the symptom of the disease, the pathophysiology behind the structural changes is complex and poorly understood. By understanding the mechanisms driving joint tissue destruction in osteoarthritis and identifying the key factors involved, new targets for therapy are emerging that will go beyond symptomatic relief to slowing or stopping the progression of osteoarthritis. <h3>Aim</h3> Thus, the aim of this study was to evaluate the effect of honey on disease progression, pain perception and inflammation in monosodium iodoacetate (MIA)-induced knee osteoarthritis in female Wistar rats. <h3>Methods</h3> Thirty, twelve-month old female Wistar rats, weighing between 200 g & 250 g, were randomly divided into five groups of six animals each. Animals in group one were not induced and served as the control, while animals in groups two to five were injected with monosodium iodoacetate in the right knee. In addition, animals in group two received normal saline (1 ml/kg b.w.), group three received arthocare (glucosamine/chondroitin sulphate 6.67/8.33 mg/kg b.w.), group four received low dose honey (250 mg/kg b.w.) while group five received high dose honey (1,000 mg/kg b.w.) and were treated for twenty one days. All animals were subjected to assessment of tactile allodynia (von Frey test), acute inflammation (knee edema), and serum biomarkers: tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF), prostaglandin E₂ (PG E₂) & cartilage oligomeric matrix protein (COMP) as well as histo-pathological assessment of the right knee joint. <h3>Results</h3> Honey (at high and low doses), significantly (p < 0.05) reduced tactile allodynia on von Frey test (60 ± 20g) in animals subjected to experimental knee osteoarthritis induced by MIA. Knee edema was also significantly (p < .05) reduced by both high and low doses of honey. Low dose honey significantly (p < .05) reduced he serum levels of TNF-α (61.5 ± 22 pg/ml), VEGF (31 ± 6.1 pg/ml) and COMP (41 ± 14 ng/ml) but, had no effect on the serum level of PG E₂. High dose honey on the other hand, only significantly reduced the serum level of TNF-α (87 ± 22 pg/ml) but, had no effect on the serum levels of VEGF, COMP and PG E₂. However, the administration of honey did not show any significant effect on histo-pathological features of the induced osteoarthritis. <h3>Conclusion</h3> Honey reversed disease progression and reduced pain perception as well as inflammation in MIA-induced knee osteoarthritis in female Wistar rats. However, honey had no effect on the histo-pathology of the knee joint.

Evaluation of therapeutic potential of Arhar honey (Cajanus cajan) in subjects with impaired glucose tolerance

Background: In Indian Medicine System, Arhar honey is believed as one of the valuable medicinal foods. Honey is being used since ancient times owing to its functional properties and nutritive value. Aims: We aimed to evaluate the effect of honey supplementation on fasting blood glucose (FBG) level and lipid profile of the subjects with impaired glucose tolerance (IGT) and healthy subjects. Methods: The selection of subjects was done through oral glucose tolerance test (World Health Organization/Food and Agriculture Organization, 1998). On the basis of blood glucose level, 28 subjects were selected by purposive random sampling and were divided into two groups. Group I included normal healthy subjects having FBG in the range of 70–100 mg/dl (n = 14), and Group II was comprised of subjects having FBG level between 100 and 125 mg/dl falling in the category of IGT (n = 14). 70 g honey was administered to subjects in both the groups on an empty stomach for 60 days and its effect on body weight, body mass index (BMI), blood pressure, FBG, glycated hemoglobin (HbA1c), and lipid profile was observed. Results: Honey administration lowered blood glucose levels and lipid levels in subjects with IGT and normal healthy subjects. Other benefits of honey administration include an increase in high-density lipoprotein cholesterol levels and a reduction in BMI. Conclusion: Consumption of honey has shown a reduction in body weight, BMI, FBG, HbA1c, and improvement in lipid profile in IGT subjects.

Modulating the Metabolic Activity of Silkworm, Bombyx mori L. with the Supplementation of Honey and Protein Sources

The present study aimed to assess the effect of honey obtained from different bee species and protein sources on the metabolic changes in mulberry silkworm, Bombyx mori L. The results of experiment showed that oral administration of honey and protein sources to silkworm larvae during late-age instars daily once significantly changed metabolic processes. Among the various treatments and concentrations tested, 5 per cent Trigona iridipennis honey significantly increased the carbohydrate in haemolymph (16.71 mg/mL) and fat body (12.38 mg/g) over all other treatments. The lipid content in haemolymph (23.03 mg/mL) and fat body (45.38 mg/g) of silkworm were also found to be superior in the larval batch treated with 5 per cent T. iridipennis. Apart from this, the same treatment was showed statistical superiority in increasing the activity of enzymes viz., aspartate aminotransferase (269.78 µg/mL) and alanine aminotransferase (563.25 µg/mL), which play an important role in protein metabolism.

Ginger honey affects cortisol, estrogen and glutathione levels; preliminary study to target preconceptional women

ObjectiveThis study was aimed to determine the effect of ginger honey supplementation on cortisol, glutathione, and estrogen levels. The study was conducted on mice that had not yet experienced conception, and prior stress induction was carried out so that they could be continued for human trials at the preconception stage and subjects who experienced mild stress. MethodIt was an in vivo study, pretest–posttest control group design. The sample of this study was 2–3 months female Balb/c mice, divided into negative control and ginger honey intervention as much as 28mg/20g BW for 14 days—the ELISA method used to examine cortisol hormone, glutathione levels, and estrogen levels. The mice chosen were those that had never experienced conception, and before the intervention, swimming activities were carried out on the mice until they showed symptoms of stress. ResultsResults show 42mg/20g BW of ginger honey administration for 14 days increased 1.892 ng/dl of cortisol (p = 0.165), increased 2.438 ng/dl of glutathione (p=0.002), and also increased 22.754ng/ml estrogen levels in induced stress Balb/c female mice (p=0.001). ConclusionGinger honey did not affect reducing cortisol levels but increasing glutathione and estrogen levels significantly. Ginger honey supplements are the potential to use as complementary therapies.

Effect of in ovo Honey Administration on Fertility and Post Hatch Performance of Broiler Chickens

Effect of in ovo Honey Administration on Fertility and Post Hatch Performance of Broiler Chickens

Honey administration in laying pullets during hot-dry season does not alter lipids and vitamin A content of eggs

An experiment was carried out to determine the effect of honey on lipid profile and vitamin A content of eggs from laying pullets during hot-dry season. One hundred and twenty laying pullets (aged 28 weeks) used were randomly assigned to 3 doses of honey: 0 (0H), 10 (10H) and 20ml honey (20H) per litre water for 16 weeks. Each treatment group consists of 4 replicates with 10 birds per replicate. During a 16-week experimental period, data on egg cholesterol, HDL, LDL, VLDL and vitamin A were determined and subjected to one-way analysis of variance. Egg cholesterol, HDL, LDL, VLDL and vitamin A concentrations were not significantly (P&gt;0.05) affected by dosage of honey in drinking water. Birds on 0H, 10H and 20H recorded 1163.8, 1154.7 and 1160.0 mg/dL respectively for egg cholesterol and 129.8, 151.0 and 123.3 IU vitamin A, respectively. In conclusion, administration of honey in drinking water of laying pullets to combat heat stress during hot-dry season may not affect egg lipids and vitamin A content.

Tualang Honey Improves Memory and Prevents Hippocampal Changes in Prenatally Stressed Rats.

This study investigated whether the alterations in memory and hippocampus morphology and levels of malondialdehyde (MDA) and N-methyl-D-aspartate (NMDA) receptor in the hippocampus of adult rats after prenatal stress could be prevented by administration of Tualang honey (TH). Twenty-four pregnant rats were randomly grouped into a control group (C), a stress group (S), and a stress group treated with TH. Eight male pups from each group were randomly chosen and they were sacrificed at eight or ten weeks of age following the novel object recognition test. Their brains were removed and histological changes and levels of MDA and NMDA receptors in the hippocampus were determined. The offspring from TH group showed significantly increased preference index (p<0.05) with higher neuronal number compared to S group. A significantly lower level of MDA and NMDA receptors were shown in TH group (P<0.01; P<0.05 respectively) compared to S group. The parameters investigated were not significantly different between C and TH groups. The study has shown that memory alteration, changes in hippocampus histology, MDA and NMDA receptor levels could be prevented by TH administration during prenatal stress. The results suggest the beneficial effects of Tualang honey in prenatally stressed rat offspring.

The effect of dietary honey prebiotic on microbiota diversity in the digestive tract of Nile tilapia ( Oreochromis niloticus ) and its growth performance

Prebiotic administration is one method to increase growth performance, improve intestinal microbiota composition, inhibit pathogen growth and induce immunity in fishes. Honey consists of oligosaccharides as the major content that can be used as a prebiotic. This study investigated the effects of administration of dietary prebiotic from honey at different doses on growth performance, digestive enzyme activities, intestinal microvilli, short-chain fatty acid contents and microbiota diversity in the digestive tract of Nile tilapia (Oreochromis niloticus). Four diet treatments were applied, namely control (without honey administration) and honey administration at 0.25%, 0.5% and 1% doses (g/kg diet). Nile tilapia weighing 20 ± 1.72 g were reared with these diet treatments for 30 days. Results demonstrated that honey prebiotic administration could increase growth performance; provide a lower feed conversion ratio; induce digestive enzyme activities (amylase, protease and lipase); increase microvilli length, perimeter ratio, density of intestinal microvilli and short-chain fatty acid contents (propionate, iso butyrate, iso-valerate and N-valerate); and improve microbiota diversity in the digestive tract of Nile tilapia at the best dietary dose of 1%.

Effect of Trigona honey on Blood glucose levels Diabetes Mellitus In Balb/c Mice

Introduction Diabetes Mellitus (DM) is a multicultural metabolic disease characterized by hyperglycemia due to abnormalities in insulin secretion, insulin action, or both. Abnormalities in insulin secretion or action cause problems in the metabolism of carbohydrates, fats, and proteins. DM Type II is characterized by the occurrence of insulin resistance in body tissues. Trigona honey is rich in phenolic compounds because it is food collected by bees from plants. The total phenolic content in honey is highly correlated with antioxidant activity. In this study, we aimed to see the different effects of trigona honey (Tetragonula sp) on plasma insulin and blood glucose levels in mice with diabetes mellitus. Methods. Balb/c mice (n= 28) were randomly assigned into the control group (n=7), negative control, positive control, and intervention group which received a daily intake of trigona honey (n= 14). Results. showed that administration of trigona honey can increase administration of the metformin. This is caused because honey contains high antioxidants and contains bioactive compounds such as alkaloids, flavonoids, triterpenoids, and phenol compounds. Conclusion. Daily consumption of trigona honey has a remarkable potential to decrease the blood glucose, thus it can contribute to the prevention of the diabetes mellitus development.

Tualang Honey Ameliorates Hypoxia-induced Memory Deficits by Reducing Neuronal Damage in the Hippocampus of Adult Male Sprague Dawley Rats.

A growing body of evidence indicates that hypoxia exposure causes learning and memory deficits. An effective natural therapeutic approach has, however, not been explored widely. Our previous studies found that Tualang honey administration protected learning and memory functions in ovariectomized rats. Therefore, the present study investigated its efficacy in ameliorating hypoxia-induced memory deficits in adult male Sprague Dawley rats. The rats were divided into four groups: i) Normoxia treated with sucrose (n=12), ii) Normoxia treated with Tualang honey (n=12), iii) Hypoxia treated with sucrose (n=12), and iv) Hypoxia treated with Tualang honey (n=12). Tualang honey (0.2 g/kg/BW) and sucrose (1 mL of 7.9%) supplementations were administered orally to the rats daily for 14 days. Then the hypoxia groups were exposed to hypoxia (~11%) for 7 days, while the normoxia groups were kept in normal conditions. Following exposure to hypoxia, the rats' memories were analyzed using a novel object recognition task and T-maze test. The data revealed that rats exposed to hypoxia showed significant impairment in short-term memory (STM), spatial memory (p<0.01), and long-term memory (LTM) when compared to the normoxia group. Hypoxia rats treated with Tualang honey showed significant improvement in STM, LTM, and spatial memory (p<0.05) compared with those treated with sucrose (p<0.05). Tualang honey also reduced neuronal damage in the hippocampus of adult male Sprague Dawley rats exposed to hypoxia. It is suggested that Tualang honey pretreatment has protective effects against hypoxia-induced memory deficits, possibly through its antioxidant contents.

The Effects of Honey Administration on Petrol-Induced Oxidative Stress and Hepatotoxicity in Sprague-Dawley Male Rats

Background: Exposure to petrol is common among individuals working in oil refineries, oil fields, filling stations, automobile mechanical workshops. Exposure to petrol is associated with a number of health hazards including hepatotoxicity and oxidative stress. Honey is rich in many antioxidants such as catalase, flavonoids, thiamine, riboflavin, pyridoxine, pantothenic acid, ascorbic acid and nicotinic acid and was recently reported to reduce petrol-induced hepatotoxicity and nephrotoxicity. Materials and Methods: A total of 32 male Sprague-Dawley rats were randomly divided into four groups: control group (exposed to ambient air daily), petrol exposed group

THE EFFECTS OF HONEY ADMINISTRATION ON SOLUABLE FMS-LIKE TYROSINE KINASE (SFLT-1), SOLUBLE ENDOGLIN (S-ENG), VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) SERUM LEVELIN THE RAT MODEL OF PREECLAMPSIA

Objective: To find out the effect of honey administration on changes in antiangiogenic and proangiogenic serum levels in the rat model of preeclampsia. This study is the first research that examines the effect of honey on preeclampsia especially on proangiogenic and antiangiogenic factors&#x0D; Methods: This study uses analytic research with a quasi-experimental design in laboratory rats (Rattus Norvegicus) pregnant females given honey with concentration. The treatment of all samples was carried out simultaneously and during the treatment it was observed using the type of postest only control group design.&#x0D; Results: Honey administration significantly reduced sFlt-1 levels in preeclampsia rats, and a greater dose of honey had an effect on strengthening the effect of honey in reducing sFlt-1 levels. Honey administration significantly increased VEGF levels in preeclampsia mice (p = 0.034). Honey administration significantly decreased s-Eng levels in preeclampsia mice, and administration of a larger dose of honey had an effect on strengthening the effect of honey in reducing s-Eng levels (p = 0.012).&#x0D; Conclusion: The honey administration on rat’s model of preeclampsia may reduce the antiangiogenic level sFlt-1 and sEng dan increase VEGF level as the pro-angiogenic

Erratum to "Evaluating the Effects of Chronic Administration of Natural Honey on the Development of Dependence on Morphine in the Male Rats"

Erratum to "Evaluating the Effects of Chronic Administration of Natural Honey on the Development of Dependence on Morphine in the Male Rats"

Protective effect of citrus honey toward the histopathological and biochemical nephrotoxicity damages of gentamicin–induced in local rabbits Oryctolagus cuniculus

Drugs induced nephrotoxicity now considered as an essential reason for kidney failure. Aminoglycoside anti-infection agents, for example, gentamicin, which causes ototoxicity and nephrtotoxicity as a side effect , this research is centered around the utilization of natural materials as an antioxidants against the lethal oxidative activity that applied on the kidney cells ,the most imperative one of these materials is the honey. This work aimed to assess the defensive impact of citrus honey against the histopathological and biochemical harms of gentamicin induced nephrotoxicity .24 locally breed rabbits (Oryctolagus cuniculus) were used and divided into 4 study groups (6 rabbits per each group), group 1 received I.P daily dose of normal saline (control), group 2 received (80 mg/kg/day) I.P dose of gentamicin, group 3 received (80mg/kg/day) of gentamicin I.P dose in combination with oral dose of Citrus Honey625 mg/kg/day for 14 days and group 4 received (80mg/kg/day) of gentamicin I.P dose in combination with extra dose of Citrus Honey orally for 14 days. All animals (at 15th day) were anesthetized by ether and sacrificed; blood samples were gathered for the subsequent measurement of the serum creatinine, urea and albumin while an isolated kidney was kept in 10 % of formaldehyde for the histopathological examination. The results demonstrated that gentamicin causes nephrotoxicity showen by elevation of serum level of creatinine, urea and a decrease in the serum albumin. While the administration of honey in combination with gentamicin reduced the nephro-toxic effect of gentamicin that represented by a reduction of the serum creatinine and urea with improvement of the kidney histological findings.This study concluded that, honey decreased nephrotoxic effect of gentamicin.