Adequate Protein Research Articles

Metabolic alterations and cellular responses to β-Hydroxybutyrate treatment in breast cancer cells

BackgroundThe ketogenic diet (KD), based on high fat (over 70% of daily calories), low carbohydrate, and adequate protein intake, has become popular due to its potential therapeutic benefits for several diseases including cancer. Under KD and starvation conditions, the lack of carbohydrates promotes the production of ketone bodies (KB) from fats by the liver as an alternative source of metabolic energy. KD and starvation may affect the metabolism in cancer cells, as well as tumor characteristics. The aim of this study is to evaluate the effect of KD conditions on a wide variety of aspects of breast cancer cells in vitro.MethodsUsing two cancer and one non-cancer breast cell line, we evaluate the effect of β-hydroxybutyrate (βHb) treatment on cell growth, survival, proliferation, colony formation, and migration. We also assess the effect of KB on metabolic profile of the cells. Using RNAseq analysis, we elucidate the effect of βHb on the gene expression profile.ResultsSignificant effects were observed following treatment by βHb which include effects on viability, proliferation, and colony formation of MCF7 cells, and different effects on colony formation of MDA-MB-231 cells, with no such effects on non-cancer HB2 cells. We found no changes in glucose intake or lactate output following βHb treatment as measured by LC-MS, but an increase in reactive oxygen species (ROS) level was detected. RNAseq analysis demonstrated significant changes in genes involved in lipid metabolism, cancer, and oxidative phosphorylation.ConclusionsBased on our results, we conclude that differential response of cancer cell lines to βHb treatment, as alternative energy source or signal to alter lipid metabolism and oncogenicity, supports the need for a personalized approach to breast cancer patient treatment.

#2420 A comparative analysis of food intake according to the degree of dietary compliance among Korean hemodialysis patients

Abstract Background and Aims Food intake management plays a crucial role in the treatment of hemodialysis (HD) patients. Maintaining a balanced diet involves adequate protein intake, sufficient calorie consumption, and restriction of water, sodium, and potassium intake. This study aims to highlight the significance of balanced dietary management by comparing and analyzing differences in food intake patterns among Korean HD patients based on the levels of dietary adherence. Throughout our analysis, we suggest the importance of balanced food intake management for HD patients. Method The data were collected through a survey conducted among 442 HD patients across 18 university hospitals nationwide. The survey employed the dietary adherence and the food intake frequency questionnaire (FFQ). The collected data were analyzed using SPSS 26.0. The analysis method was K-means cluster analysis to classify the degree of dietary adherence into high-adherence and low-adherence. In addition, cross-analysis and independent samples t-test were utilized to compare differences between the groups in demographic characteristics, general health and nutritional status, laboratory data, and food intake frequency. Results Gender distribution revealed 257 males (59.4%) and 176 females (40.6%). The age distribution was as follows: 50-64 years old, 179 individuals (40.8%); 30-49 years old, 97 individuals (22.1%); 65–74 years old, 97 individuals (22.1%); 75 years and above, 52 individuals (11.8%). BMI determination resulted in the following order: underweight - normal in 207 people (48.8%); obese - severely obese in 123 people (29.0%); and overweight in 94 people (22.2%). Subjective Global Assessment (SGA) indicated that 296 individuals (73.3%) were in good nutritional status, while 108 individuals (26.7%) were in fair nutritional status. The duration of HD was distributed as follows: 135 patients (32.1%) for 1–3 years, 103 patients (24.5%) for 5–10 years, 82 patients (19.5%) for 3–5 years, and 66 patients (15.7%) for 10 years or longer. The subjects were clustered into two groups based on their dietary compliance: high adherence and low adherence. The dietary compliance scores, on a scale of 22 points, were 13.63 ± 1.475 for the high adherence group and 9.15 ± 1.681 for the low adherence group. In the high adherence group, uric acid (p < 0.01), serum creatinine (p < 0.01), intact PTH (p < 0.01), TIBC (p < 0.05), and glucose (p < 0.05) were significantly lower than those parameters in the low adherence group. The frequency of daily food intake was also analyzed according to the dietary adherence. The high adherence group showed significantly higher frequencies in rice(p < 0.001), white fish(p < 0.05), low-potassium vegetables(p < 0.01), mushrooms(p < 0.01), low-fat milk(p < 0.01), liquid yogurt(p < 0.05) compared to the low adherence group. On the other hand, the high adherence group showed lower consumptions of foods, such as gimbap, bibimbap (p < 0.05), noodle soup(p < 0.01), bread(p < 0.05), fruit juice(p < 0.05), and tea(p < 0.01). Conclusion The high-adherence group exhibited significantly satisfactory blood parameters and food intake status, highlighting the pivotal role of dietary compliance. Consequently, adhering to dietary guidelines is essential for ensuring sufficient nutrient intake and overall health among HD patients.

Discussion on protein recommendations for supporting muscle and bone health in older adults: a mini review.

Muscle and bone tissues are interconnected, and both rely on an adequate protein intake. Recommendations for protein intake for older adults specifically vary across countries. The purpose of this narrative review is to discuss the existing evidence for protein recommendations for supporting muscle and bone health in older adults and to evaluate if a protein intake above the current population reference intake (PRI) for older adults would be scientifically justified. First, this review summarizes the protein recommendations from bodies setting dietary reference values, expert groups, and national health organizations. Next, relevant studies investigating the impact of protein on muscle and bone health in older adults are discussed. In addition, the importance of protein quality for muscle and bone health is addressed. Lastly, a number of research gaps are identified to further explore the added value of a protein intake above the PRI for older adults.

The Impact of Bariatric Surgery on Nitrogen Balance at Six months Post-surgery.

Obesity, defined as abnormal or excessive fat accumulation that presents a risk to health, rose from 8.6 to 10.5% in Singapore's residents. Bariatric surgery, the primary treatment for severe obesity, induces fat and muscle loss. Adequate protein intake is vital for preventing muscle loss. This study examines nitrogen balance in individuals with obesity pre- and post-surgery. Sixteen participants with severe obesity (BMI ≥ 32.5kg/m2) undergoing bariatric surgery (14 sleeve gastrectomy, 2 Roux-en-Y gastric bypass) and 20 normal-weight controls (BMI < 25kg/m2) were recruited. Nitrogen balance, calculated from dietary protein intake and urine nitrogen excretion, was assessed. Participants with obesity were re-evaluated 6months post-surgery. Data were analyzed using parametric methods. At baseline, controls had a BMI of 20.8 ± 2.1kg/m2; those with obesity had 40.9 ± 7.3. Daily calorie and protein intake for participants with obesity were not statistically significantly different from controls (calorie intake at 1467 ± 430 vs. 1462 ± 391kcal, p = 0.9701, protein intake 74.2 ± 28.7 vs. 64.6 ± 18.3g, p = 0.2289). Post-surgery, BMI, fat-free mass, fat mass, total energy intake, carbohydrate, and protein intake decreased significantly (p < 0.01). Protein oxidation and urine nitrogen excretion did not change after bariatric surgery. However, nitrogen balance significantly reduced from 2.62 ± 5.07 to - 1.69 ± 5.07g/day (p = 0.025). Dietary protein intake is inadequate in individuals with obesity at 6months post-bariatric surgery and contributes to a state of negative nitrogen balance.

The impact of nutrition on psycho-affective status in an older Cretan population: a cross-sectional study.

Cognitive and mood status influence both personal and social daily activities, with great impact on life quality, particularly among the elderly population. This cross-sectional study aimed to investigate the psycho-affective status concerning eating habits within an elderly population of the Chania area in Crete, Greece. Cognitive status was assessed in 101 elderly subjects through the Mini-Mental State Examination (MMSE), and mood was evaluated using the Hospital Anxiety and Depression Scale (HADS). Nutritional status was assessed using a validated food frequency questionnaire. Multivariable statistical analysis, after adjustment for age, marital status, education, and comorbidity, highlighted among males a positive association of the MMSE score with vegetable consumption (RR 1.18; 95%CI 1.03‒1.34) and a negative association with potato consumption (RR 0.83; 95%CI 0.72‒0.95). Conversely, among females, no statistically significant association was observed for any food. Further, among males, a protective effect on affective status was identified for chicken meat (RR 0.45; 95%CI 0.27‒0.77), fish (RR 0.41; 95%CI 0.21‒0.82), fruit (RR 0.70; 95%CI 0.52‒0.94), cereals (RR 0.67; 95%CI 0.53‒0.87), and cheese (RR 0.78; 95%CI 0.63‒0.97) consumption. Among females, the adjusted model showed a significant detrimental effect of vegetable consumption (RR 1.33; 95%CI 1.02‒1.73). A predominantly vegetable-based diet-with the notable exception of fruits and legumes-was associated with better cognitive status in males, albeit not in females. A higher intake of fruit, as well as fish, chicken meat, and cheese among males was associated with a better affective status, indicating that adequate protein supply may play a role in maintaining emotional balance.

Pulses in Uttarakhand: Has Their Potential Been Tapped Enough to Attain Food and Nutritional Security?

Background: Malnutrition is a global health crisis and Uttarakhand state is no exception. Besides the implementation of various government schemes for eradication of undernutrition is at very slow pace calls for analyzing the present situation and future prospects of food crops. In this regard, Uttarakhand a hilly state with many agricultural constraints needs special attention. Pulses known for rich protein and micronutrient content can serve as an excellent source for mitigating protein deficiency and attaining food and nutrition security. Various pulses in different cropping pattern are being grown in the state. The current study aims at exploring the dynamics, diversification and decomposition of pulses in Uttarakhand and their role in providing food and nutritional security. Methods: Secondary data related to area under pulses, production and yield were taken from official website of Agriculture department, Government of Uttarakhand. The data gaps were filled through linear interpolation. Production of total pulses was linearly extrapolated to estimate pulses production for 2035-36. Growth and instability, diversification and decomposition of pulses were analyzed. The Food and Nutritional security statistics were taken from directorate of Economics and Statistics, Government of Uttarakhand and explored in relation to pulses. Result: It was found that the state is faring well in production of pulses as an increase in absolute area, production, yield and proportion of pulses in gross sown area was observed. High pulse diversification was seen. However, a decline in all major pulse-based cropping systems was seen. Moreover, in the state, where 31% of the population lacks adequate protein intake, there is a pressing need to enhance the availability, accessibility and absorption of protein-rich pulses, particularly for vegetarians. Additionally, it is essential to reevaluate the state’s food and nutrition security status in alignment with the updated guidelines from ICMR-NIN in 2020.

How Can We Improve the Appetite of Older Patients on Dialysis in Japan?

It is necessary to ensure adequate energy and protein intake in dialysis patients. However, in addition to the decline in dietary intake in older dialysis patients due to aging, the rate of anorexia is high in dialysis patients, which increases the risk of protein–energy wasting (PEW), sarcopenia, and frailty. There are many causes of anorexia in dialysis patients, including older dialysis patients, and approaches to improve the appetite of such patients have been reported; however, there has been no established approach to improve appetite adequately. Therefore, a key practical goal is to identify anorexia early and implement timely interventions before weight loss occurs. Appetite assessment tools and weight loss assessments are helpful for the screening and early identification of anorectic signs. Nutritional interventions include reducing dietary restrictions, using oral nutritional supplements, and intradialytic parenteral nutrition, as well as replenishing energy, protein, and zinc to prevent the development of nutritional disorders among older dialysis patients. Appetite assessments, early intervention, and dietary and nutritional counseling are key to improving appetite in these patients. The aging rate of dialysis patients in Japan is unprecedented globally, and I believe that this is a situation that will eventually occur in other countries as well. I discuss the factors that contribute to anorexia, especially in older dialysis patients, and Japan’s efforts to address this problem, such as the relaxation of dietary restrictions and the use of oral nutritional supplements.

Impacts of protein quantity and distribution on body composition.

The importance of meal distribution of dietary protein to optimize muscle mass and body remains unclear, and the findings are intertwined with age, physical activity, and the total quantity and quality of protein consumed. The concept of meal distribution evolved from multiple discoveries about regulating protein synthesis in skeletal muscle. The most significant was the discovery of the role of the branched-chain amino acid leucine as a metabolic signal to initiate a post-meal anabolic period of muscle protein synthesis (MPS) in older adults. Aging is often characterized by loss of muscle mass and function associated with a decline in protein synthesis. The age-related changes in protein synthesis and subsequent muscle atrophy were generally considered inevitable until the discovery of the unique role of leucine for the activation of the mTOR signal complex for the initiation of MPS. Clinical studies demonstrated that older adults (>60 years) require meals with at least 2.8 g of leucine (~30 g of protein) to stimulate MPS. This meal requirement for leucine is not observed in younger adults (<30 years), who produce a nearly linear response of MPS in proportion to the protein content of a meal. These findings suggest that while the efficiency of dietary protein to stimulate MPS declines with aging, the capacity for MPS to respond is maintained if a meal provides adequate protein. While the meal response of MPS to total protein and leucine is established, the long-term impact on muscle mass and body composition remains less clear, at least in part, because the rate of change in muscle mass with aging is small. Because direct diet studies for meal distribution during aging are impractical, research groups have applied meal distribution and the leucine threshold to protein-sparing concepts during acute catabolic conditions such as weight loss. These studies demonstrate enhanced MPS at the first meal after an overnight fast and net sparing of lean body mass during weight loss. While the anabolic benefits of increased protein at the first meal to stimulate MPS are clear, the benefits to long-term changes in muscle mass and body composition in aging adults remain speculative.

Assessing the Nutrient Intake, Energy Expenditure and Physical Activity Level of Obese and Non-obese Women in Hisar District, India: A Cross Sectional Study

The present study was conducted on thirty obese and thirty non-obese women (30-45 years) of Hisar district, Haryana to assess their mean nutrient intake, adequacy of intake of different nutrients, energy expenditure and physical activity level. The subjects did not exhibit any health complications and, overall, were in a state of good health. It was found that the mean intake of energy, protein, fat, folic acid and magnesium by respondents was comparable to Recommended Dietary Allowances (RDA) i.e 98 to 129 per cent of RDA. But when assessed on parameter of adequacy it was found that only 50 to 53.3 percent of respondents consumed adequate protein. Intake of most of the nutrients (iron, β-carotene, folic acid) under study, by them, was marginally adequate to inadequate when compared to the recommended values (with few exceptions). Calcium intake of 50 per cent of non-obese women was adequate while iron intake of 66.7 per cent of them was 50 to 74.9 per cent of RDA. All the obese and non-obese women were sedentary workers and consumed more energy than their requirement. This low intake in their nutrient intake may be due to inadequate intake of protective foods and it was felt that they need awareness regarding balanced diet and endorse adoption of a healthy life style by including minimum of 30 minutes of physical activity in their daily routines.

Phase-specific outcmes of arginine or branched-chain amino acids supplementation in low crude protein diets on performance, nutrient digestibility, and expression of tissue protein synthesis and degradation in broiler chickens infected with mixed Eimeria spp.

A 35-d study investigated the impact of dietary supplementation with Arginine (Arg) or branched-chain amino acids (BCAA) of broilers receiving low-protein diets whilst infected with mixed Eimeria species. All birds were given the same starter (d0-10) and finisher (d28-35) diets. The 4 grower diets used were a positive control (PC) with adequate protein (18.5%), a low protein diet (NC;16.5% CP), or the NC supplemented with Arg or BCAA. Supplemental AA was added at 50% above the recommended levels. The treatments were in a 4 × 2 factorial arrangement, with 4 diets, with or without Eimeria inoculation on d14. Birds and feed were weighed after inoculation in phases: prepatent (d14-17), acute (d18-21), recovery (d22-28), and compensatory (d29-35). Ileal digesta, jejunum, and breast tissue were collected on d21, 28, and 35. There was no diet × Eimeria inoculation on growth performance at any phase. Infected birds weighed less and consumed less feed (P < 0.05) in all phases. In the prepatent and acute phases, birds on the Arg diets had higher weight gain (P < 0.05) and lower FCR, similar to PC, when compared to NC and BCAA-fed ones. Infection reduced AA digestibility on d21 and 28 (Met and Cys). However, birds that received supplemental AA had higher digestibility (P < 0.05) of their respective supplemented AA on d 21 only. Infected birds had lower (P < 0.05) BO + AT and higher PEPT1 expression on d21. There was a diet × Eimeria interaction (P = 0.004) on gene expression at d28; 4EBP1 genes were significantly downwardly expressed (P < 0.05) in birds fed Arg diet, irrespective of infection. Infected birds exhibited an upward expression (P < 0.05) of Eef2 on d21 and d28 but experienced a downward expression on d35. Supplemental Arg and BCAA had variable effects on growth performance, apparent ileal AA digestibility, and genes of protein synthesis and degradation, but the effect of Arg on promoting weight gain, irrespective of the Eimeria challenge, was more consistent.

The Correlation Between Protein Intake And Muscle Mass in Breast Cancer Patients at Dr. H. Abdul Moeloek Regional Hospital

Breast cancer is the second-leading cause of death among women. Since protein serves as a building ingredient for cells, muscles, and tissues, eating enough of it is thought to be essential for gaining muscle mass. The cornerstone for cancer patients to maintain and/or gain muscle mass will be an adequate protein intake. In this study, breast cancer patients' muscle mass was measured and a 24-hour recall of their weekdays and weekends was conducted. The average amount of protein consumed by participants was 31.22 grams, and the average percentage of muscle mass was 24.02%. The purpose of this study is to ascertain how breast cancer patients' protein consumption and muscle mass relate to one another. This study uses quota sampling as the sample method and a cross-sectional design. There were fifty-six responders in the sample. This research uses the Spearman rank. The normality test results show that the significance value for muscle mass is p value = 0.016 (p &lt; 0.05), it can be concluded that the data is distributed abnormally. Meanwhile, the normality test results for protein intake p-value = 0.200 (p &gt; 0.05) can be concluded that the data is normally distributed, so it is continued with the Spearman rank correlation test. Based on the results of the Spearman rank correlation test, a correlation coefficient value of 0.488 was obtained with a significance p-value of 0.0001. So there is a positive significant correlation between protein intake and muscle mass in breast cancer sufferers at Dr. H. Abdul Moeloek Regional Hospital of Lampung Province p value = 0.0001 (p&lt;0.05).

Nutrition adequacy in the late period of the acute phase is associated with a lower risk of 30-day mortality in critically ill patients: A prospective cohort study.

The provision of nutrition support for critically ill patients in the early phase of intensive care unit (ICU) admission plays a vital role in their recovery. However, there is still debate regarding the impact of nutrition adequacy of critical illness phases. We aimed to investigate whether nutrition adequacy in the acute phase (early and late periods) is associated with 30-day mortality in critically ill patients. We prospectively collected nutrition and clinical data from critically ill patients receiving exclusive enteral nutrition (EN) within the first 10 days of ICU admission. EN was classified as adequate when ≥80% of the prescribed EN was administered. Directed acyclic graphs were constructed to identify the minimum set of adjustment variables required to control for confounding factors. The relationships between energy and protein intake and 30-day mortality were assessed using the Cox regression analysis. A total of 119 patients were evaluated (70 years old, 56.3% male, and 68.1% with medical admission). The 30-day mortality rate was 23%. After adjusting for confounders, in the late period (days 5-10), energy adequacy (hazard ratio [HR] = 0.960; 95% CI, 0.937-0.984) and protein adequacy (HR = 0.960; 95% CI, 0.937-0.982) were predictors of 30-day mortality. No associations were observed in the early period (days 1-4) of the acute phase. In critically ill patients, nutrition adequacy (≥80% EN) during days 5-10 in the ICU was associated with a lower risk of 30-day mortality.

Maternal Caregiving Capabilities are Associated with Energy-Protein Adequacy of Children with Stunting in Central Java, Indonesia

Indonesian children suffer from stunting because of a chronic lack of energy and protein intake. Maternal caregiving capabilities are skills and attributes of the mother which determine their ability to use resources for positive nutrition to support their children's health. This study aimed to analyse the association between maternal caregiving capabilities and energy-protein adequacy among children with stunting. This study used a cross-sectional correlation design. The data was collected using a maternal caregiving capabilities questionnaire and 24-hour food recall in 130 mothers of children aged 2 to 5 years with stunted growth, registered at Puskesmas. The study found a significant association between maternal caregiving capabilities and a child's energy and protein adequacy. As mothers' maternal caregiving capabilities increased, their capacity to provide adequate energy and protein for their children increased. We believe that community health nurses can promote health and empower mothers to increase their capability to meet the nutritional needs of children.

Nutrient intake and stunting in children aged 2-5 years in a slum area of Jakarta

Background Stunting is one of the problems that occurs in children who live in slum areas. Inadequate nutrient intake has been associated with stunting in children. Objective To assess nutrient intake and analyze the differences between stunted and non-stunted children aged 2-5 years. Methods This comparative cross-sectional study compared nutrient intake and stunting among children 2-5 years in slum areas in Kebon Bawang Village North Jakarta. Subjects’ nutrient intake was assessed using the Semiquantitative-Food Frequency Questionnaire (SQ-FFQ). To analyze differences in subject characteristics and nutrient intake in the stunted and non-stunted groups, Chi-square, Mann-Whitney test, and independent T-test were used. Results From a total of 42 respondents, the characteristics of subjects were not significantly different between the stunted and non-stunted groups, in age (P=0.120), gender (P=0.126), maternal occupation (P=0.729), or maternal education (P=0.127). The stunted group had significantly lower intake of energy (P=0.003), carbohydrates (P=0.024), protein (P=0.005), and fat (P=0.001) than that of the non-stunted group. However, the majority of subjects had protein adequacy above the sufficiency level in both groups (P=0.638), while significantly more subjects in the stunted group had insufficient carbohydrate adequacy than in the non-stunted group (P=0.032). Conclusion Overall, nutrient intake in the stunted group is lower than that of the non-stunted group. Protein adequacy is above sufficient for most subjects in both groups, while the significantly more stunted subjects have insufficient carbohydrate adequacy. In carrying out interventions, it is necessary to consider fulfilling a balance of nutrients, especially macronutrients.

Crude protein content in diets associated with intestinal microbiome and metabolome alteration in Huanjiang mini-pigs during different growth stages.

Adequate crude protein (CP) content in diets plays a crucial role in the intestinal health of the animal. This study investigated the impacts of CP content in diets on the intestinal microbiome and metabolome profiles in growing Huanjiang mini-pigs. A total of 360 pigs with similar body weight (BW) were allocated for three independent feeding trials based on three different BW stages, including (i) 5-10 kg BW, diets consisting of 14, 16, 18, 20, and 22% CP content; (ii) 10-20 kg BW, diets consisting of 12, 14, 16, 18, and 20% CP content; and (iii) 20-30 kg BW, diets consisting of 10, 12, 14, 16, and 18% CP content. These experiments lasted 28, 28, and 26 days, respectively. The results showed that the Shannon and Simpson indices were decreased (p < 0.05) in the ileum of pigs in response to the 14-18% CP compared with the 20% CP content at 5-10 kg BW stage, while diets containing 12 and 14% CP had higher Chao1 (p < 0.05) and Shannon (p = 0.054) indices compared with 18% CP at 20-30 kg BW stage. Compared with the 20% CP, the diet containing 16% CP displayed an increasing trend (p = 0.089) of Firmicutes abundance but had decreased (p = 0.056) Actinobacteria abundance in the jejunum at 5-10 kg BW stage. In addition, a diet containing 16% CP had higher Lactobacillus abundance in the jejunum and ileum compared with the 18, 20, and 22% CP, while had lower Sphingomonas and Pelomonas abundances in the jejunum and Streptococcus abundance in the ileum compared with the diet containing 22% CP (p < 0.05). Diets containing lower CP content altered differential metabolites in the small intestine at the early stage, while higher CP content had less impact. These findings suggest that a diet containing lower CP content (16% CP) may be an appropriate dietary CP content for 5-10 kg Huanjiang mini-pigs, as 16% CP content in diet has shown beneficial impacts on the intestinal microbiome and metabolome profiles at the early growth stage of pigs.

The Effect of Ketogenic Metabolic Therapy on Recurrent High-Grade Gliomas: Case Series

High-grade gliomas represent the most prevalent primary malignant brain tumors in the adult population. Despite extensive efforts dedicated to advance in therapeutic modalities, treatment options remain very limited in this field, leading to a worse prognosis for affected patients. Moreover, a considerable number of cases exhibit refractory disease following the initial management. Ketogenic Metabolic Therapy (KMT) has emerged as a promising adjunctive approach for recurrent high-grade gliomas, offering them a synergistic effect enhancement of the effectiveness of conventional therapies against this type of cancer. The underlying hypothesis relies on the use of high-fat, low-carbohydrate diet, with adequate protein content can potentially induce metabolic reprogramming in cancer cells. Currently, the use of KMT is on the rise and has shown positive effects on the control of selected neurological symptoms and improvements in patient’s quality of life. This is a descriptive observational multicenter study (case series) that includes patients with recurrent high-grade gliomas who underwent Ketogenic Metabolic Therapy (KMT) and were supplemented with a liquid ketogenic formula (in a 3:1 ratio) as a complementary treatment to the ongoing medical regimen for recurrence. The results show a positive impact on seizure control, as well as significant improvements in cognition, memory and functionality, as reported by patients and/or their families (caregivers). Regarding the disease status, assessed by the RANO criteria, the 12-month follow-up evaluation indicated stable disease in 55% and a partial response in 20% of cases. Although the results of this work are promising, more conclusive clinical data are still needed to support the use of KMT in the treatment of high-grade gliomas.

The Relationship of Physical Activity, Energy, and Protein Intake with Nutritional Status in Adolescents at Junior High School

Indonesia is still in a nutritional emergency. Undernutrition or overnutrition is a health problem that is often found in adolescence. Nutritional problems that occur are influenced by an imbalance between physical activity and macronutrient intake in adolescents. This study aims to determine the relationship between physical activity, energy intake, and protein intake with nutritional status in adolescent girls. Observational study with a cross-sectional approach conducted in July – August 2023 at Junior High School 2 Long Bagun, Mahakam Ulu Regency, East Kalimantan. A total of 42 adolescents were included as samples based on purposive sampling by setting inclusion and exclusion criteria. Physical activity variables were measured in the International Physical Activity Questioner (IPAQ) while energy and protein intake were obtained through a 24-hour food recall form. The BMI / U formula measures nutritional status-data analysis using the Spearman Rank correlation test. Our findings show the majority of adolescents with moderate physical activity (76.2%), adequate energy intake (66.7%), and adequate protein intake (66.6%). The results of the relationship analysis showed physical activity (p = 0.001, r = 0.512), energy intake (p = 0.001, r = 0.503), protein intake (p = 0.002, r = 0.459) with the nutritional status of adolescents at Junior High School 2 Long Bagun. There is a significant relationship between physical activity, energy intake, and protein intake with the nutritional status of adolescents at Junior High School 2 Long Bagun. These findings are empirical evidence for the formation of better adolescent health programs

Tailoring the high-protein texture-modified foods for the dysphagia elderly by heating whey protein isolate-pectin complexes

Adequate dietary protein intake is an effective way to combat muscle wasting disorders in the elderly, who are highly susceptible to dysphagia due to declining physiological functions. Herein, whey protein isolate (WPI) and pectin electrostatic complexes were chosen to prepare texture-modified foods with high protein (15%, w/v) for dysphagia elderly. Firstly, the region of soluble complex formation was initially identified as pH 4.2–5.5 by determination of the zeta potential of both biopolymers. Following this, it was found that the stability of WPI-pectin complexes was significantly dependent on the increase in the amount of pectin (from 1.0 to 2.0 %, w/v). However, the viscoelasticity and the degree of WPI aggregation of these complexes after heat treatment (90 °C, 20 min) both showed the lowest level at pH 4.6 (it was fluid state) regardless of the pectin content, and the degree of protein aggregation weakened with the increase of pectin content. Additionally, the shear viscosity, yield stress, and tribological properties of the thermal complexes were also positively correlated with the degree of protein thermal aggregation. Moreover, based on the dietary texture classes developed by the International Dysphagia Diet Standardisation Initiative (IDDSI), unlike pure WPI thermally-induced gels that are attributed to level 6, the WPI-pectin thermal complexes can achieve texture class coverage from level 3 to level 6. These findings suggest that by modulating the electrostatic binding strength and positive-negative charge balance between WPI and pectin, tailored design of high-protein diets for elderly people with varying degrees of dysphagia may be achieved.

Macronutrient intakes of adolescent rowers for growth, development and sports performance

Dietary intake plays a key role in athletic performance in rowing(1). Suboptimal nutrition within the adolescent rowing population may negatively affect performance, normal growth and development, professional athlete development, and career longevity. Previous research has indicated that suboptimal carbohydrate intakes are a common issue in rowing(2). The quality of nutritional intake in adolescent rowers has seldom been explored. During moderate training, adolescent athletes should aim for 5-7g.kg-1 of carbohydrates, 1.3-1.8g.kg-1 of protein, and 20-35% energy from fat(3). This study aimed to examine the dietary intake of adolescent rowers in New Zealand and compare it with nutritional guidelines for normal growth, development, and sports performance. A cross-sectional study design involved data collection on two ‘hard’ training days, and two ‘recovery’ days from rowers (14-21 years) recruited from clubs and secondary schools around New Zealand. Participants completed four 24-hour collection periods, recording food intake, training duration and intensity. The food records were verified for accuracy, and dietary data was entered into Foodworks software for nutritional analysis. IBM SPSS software was used to calculate mean intakes for carbohydrate, protein, fat, and standard deviations. Independent t-tests were used to compare carbohydrate and protein intakes between males and females. Of the initial 40 participants, 35 fully (n = 23 females, 16.8 ± 1.9 years and n = 12 males, 17.3 ± 1.6 years) completed the study. Participants consumed 319 ± 116g (4.5 ± 1.7g.kg-1/day) of carbohydrates, 121 ± 56 g (1.7 ± 0.7 g.kg-1/day) of protein and 113 ± 46 g (1.6 ± 0.6g.kg-1/day) of fat per day. Females consumed 290 ± 80g (4.4 ± 1.3g.kg-1/day) of carbohydrates and males consumed 400 ± 78 g (5.0 ± 1.4g.kg-1/day) per day, with no significant difference between males and females intake per kilogram of bodyweight per day (p = 0.165). Minimum carbohydrate levels of 5g.kg-1 per day were only achieved by 7 females (30.4%) and 4 (33.3%) males. Females consumed significantly less protein per day, 106 ± 38g (1.6 ± 0.6 g.kg-1/day), in comparison to males who consumed 164 ± 46 grams (2.0 ± 0.5 g.kg-1/day) per day (p = 0.04). Fourteen females (60.9%) and 10 males (83.3%) consumed more than the minimum requirement of 1.3g.kg-1 of protein per day. The findings suggest that 2 out of 3 adolescent rowers in New Zealand fail to reach the minimum recommendations for carbohydrate intake(3), and males more readily meet the recommended intakes of protein when compared to females. Nutrition education for adolescent rowers in New Zealand should emphasise adequate carbohydrate and protein intakes that meet sports nutrition guidelines in order to support normal growth, development and optimised performance for these athletes.

Correlation Between The Adequacy Of Protein And Vitamin Intake With The Incidence Of Anemia In Pregnant Women

Background: Nutritional deficiencies in pregnant women can affect fetal growth and development and cause anemia during pregnancy. Anemia can be characterized by hemoglobin concentration lower than a normal boundary. This study investigates the correlation between the adequacy of protein, vitamin C, vitamin A, vitamin B12, and folic acid intake and the incidence of anemia in pregnant women at Ngoro Health Center.Research Methods: The research is analytically observational, using a cross-sectional design. The study subjects were 52 pregnant women using a purposive sampling technique. Anemia in pregnant women was obtained from the results of hemoglobin level checks recorded in the Antenatal Care (ANC) visit logbook. Meanwhile, the adequacy of protein, vitamin C, vitamin A, vitamin B12, and folic acid intake was obtained from interviews using the Semi-Quantitative Food Frequency Questionnaire (SQ-FFQ) form. The statistical correlation between categorical variables was analyzed using the chi-square test.Research Result: The study found that out of 52 pregnant women, it was known that the pregnant women's adequacy level of intake for protein (65.4%) was classified as less, vitamin C (94.2%) was classified as good, vitamin A (98.1%) was classified as good, vitamin B12 (69.2%) was classified as good, and folic acid (76.9%) was classified as good. Statistical test results showed that the adequacy of protein intake (p 0.001), vitamin B12 (p 0.035), and folic acid (p 0.021) correlates with the incidence of anemia in pregnant women.Conclusion: Based on this study, there is a significant correlation between the adequacy of protein, vitamin B12, and folic acid intake, but no correlation between the adequacy of vitamin C and vitamin A intake with the incidence of anemia in pregnant women in the working area of Ngoro Health Center, Mojokerto Regency. Pregnant women are expected to pay more attention to their nutrition intake during pregnancy.