The role of vitamins in nutrition of the Croatian population is still underestimated and insufficiently investigated although they have important protective effects in human health. The aim of this research was to examine vitamin A, thiamine, riboflavin, pyridoxine, niacin and ascorbic acid intake in 120 adults, aged 18–54 years (mean 33.8 years). The research was conducted using a 24-h recall method, and was repeated 10 times during 1 year. Participants were separated according to age into two groups (≤30 years, and > 30 years), as well as according to sex. There were no significant differences in vitamins intake between age groups. When divided by sex, significant difference in vitamins intake was found. When nutritive density was considered, significant difference was found for more vitamins regarding age, and less regarding sex. In conclusion, considering recommendations, the examined population took adequate amounts of vitamins.