Acidification Effect Research Articles

Dietary addition of compound organic acids improves the growth performance, carcass trait, and body health of broilers

IntroductionThe poultry industry constantly seeks strategies to enhance broiler growth performance and overall health. Organic acidifiers, including L-lactic acid, L-malic acid, and acetic acid, have gained attention as potential feed additives to improve animal production by modulating gut health, enhancing nutrient absorption, and supporting immune function. Despite their promising effects in other animal species, the impact of this novel compound organic acidifier on broiler performance, metabolism, and immune response has not been fully elucidated. This study aims to evaluate the effects of this compound acidifier on growth performance, serum lipid profile, antioxidant status, and immune parameters in broilers, providing insights into its potential benefits as a dietary supplement for broiler health and productivity.MethodsA total of 240 broilers were randomly divided into four groups: a control group and three treatment groups receiving 0.25%, 0.5%, or 1.0% acidifier, with six replicates of ten birds each. Over a 6-week period, various parameters were measured, including serum triglycerides, high-density lipoproteins, lysozyme, immunoglobulins (IgA, IgM), superoxide dismutase (SOD) activity, IL-2, TNF-α, and gene expressions related to lipid metabolism.ResultsOver a 6-week period, the acidifier decreased serum triglycerides and high-density lipoproteins while also enhancing growth performance. Additionally, it raised the serum levels of lysozyme, IgA, IgM, and the SOD. Additionally, IL-2 and TNF-α concentrations in the jejunum mucosa decreased. The acidifier upregulated PPARα, AMPK, FABP1 and MTTP expressions, and downregulated APOB100. Overall, the acidifier effectively improved broiler growth performance during the early development phase primarily by enhancing hepatic lipid metabolism, antioxidant capacity, and immune function.ConclusionThese results suggest that the acidifier may accelerate liver lipid metabolism in broilers by modulating the gene expression profiles involved in lipid metabolism.

Ocean acidification may contribute to recruitment failure for Bering Sea red king crab

We used semi-parametric Bayesian regression to determine whether ocean acidification or climate warming could explain declining productivity for southeast Bering Sea red king crab (Paralithodes camtchaticus). Negative effects of acidification explained ~21% of recruitment variability over 1980-2023, and ~45% since 2000. Ocean warming had a negligible effect in our analysis. Model-estimated annual mean bottom pH in the region has fallen from ~8.03 in 1980 to ~7.89 in 2023, approaching levels that reduce juvenile survival in laboratory studies. Improved model validation and better understanding of potential threshold effects on red king crab are needed to better understand the possible population-level acidification effect that we demonstrate.

Sustainable remediation of Cd-contaminated farmland through the rotation of rapeseed–rice varieties with different Cd accumulation potentials

In response to the safety risks posed by cadmium (Cd)-contaminated rice fields worldwide, a suitable production-and-restoration strategy is required for actual agricultural practices. To investigate the remediation effects of different accumulation varieties in rapeseed−rice cropping systems and their influence on Cd migration and transportation, field experiments were conducted based on different planting combinations (FWHR, conventional rice variety (HR) monoculture under fallow; FWLR, low Cd-accumulating rice variety (LR) monoculture under fallow; LOLR, LO (low Cd-accumulating rapeseed variety)-LR rotation; LOHR, LO-HR rotation; HOLR, HO (high Cd-accumulating rapeseed variety)-LR rotation; HOHR, HO-HR rotation). The study found that a rapeseed and rice rotation with appropriate varieties could reduce the rice grain Cd content, increase rice yield, and remove soil Cd without affecting agricultural production efficiency. Compared to the fallow-conventional rice pattern, various rapeseed−rice rotations reduced the Cd content of rice grains by 15%–38%, and significantly increased the available potassium (Ava-K) in the subsequent rice soil by 29.6–56.4mg/kg. The total economic benefits increased by $500–$1800 per hectare. A high accumulation variety of rapeseed and low accumulation variety of rice produced the most effective reduction in Cd levels, with a reduction rate of 38% in brown rice and an annual removal rate of 24.42g/hm2. This combination also resulted in a 29% increase in rice yield compared to the fallow-low accumulation variety rice pattern. Structural equation modeling revealed that with the combined action of crop rotation and variety selection the crop rotation directly reduced the soil available Cd or had an indirect effect by weakening the root-zone acidification effect and increasing soil Ava-P. The rotation of rapeseed and rice with carefully selected matching varieties is a feasible solution for the safe production and pollution remediation of Cd-contaminated paddy fields.

EFFECT OF DIETARY INCLUSION OF GARLIC, SYNBIOTIC, ORGANIC ACIDIFIER AND SYNTHETIC ANTIBIOTICS ON CARCASS AND CAECAL MICROBIAL LOAD OF FUNAAB ALPHA CHICKENS

This study was carried out to determine effect of garlic, synbiotic, organic acidifier and synthetic antibiotics on carcass characteristics and caecal microbial load of FUNAAB-Alpha chickens. One hundred and fifty day-old broiler chicks were randomly divided into 5 treatments of 3 replicates with 10 chicks and laid out in a Completely Randomized Design. Treatments consisted of birds on Treatment 1 (T0 - no additives), Treatment 2 (Tg - garlic at 3g/1kg feed), Treatment 3 (Ts - synbiotic at 2g/1kg feed), Treatment 4 (Toa - organic acidifier at 3g/2kg feed), Treatment 5 (Ta - antibiotics at 0.5g/1kg feed). Data obtained were subjected to one way analysis of variance. Birds on Ta showed significantly (p<0.05) increased plucked weight, eviscerated weight and dressed weight percentages similar to same parameters in birds on Ts and Tc. Weights for head, back, breast, gizzard, lungs, small intestine were also significantly (p<0.05) increased. Neck and caecum weights were significantly (p<0.05) lowered in birds on Ta. Birds on Ts showed significantly (p<0.05) increased weight for neck and it was same for kidney and caecum weights of birds on Tg. Streptococcus faecalis for birds fed Toa and Salmonella spp for birds fed Tg was significantly (p<0.05) lowered in their caecum. In conclusion, for FUNAAB-Alpha chickens, synbiotic improved carcass yield similar to antibiotic inclusion in diet; synbiotic and organic acidifier improved breast muscle similar to antibiotic inclusion in diet; additive inclusion lowered Streptococcus faecalis and Salmonella spp. population in the caecum.

Alterations of throughfall and stemflow chemistry by vegetation in China: Insights from a machine learning based meta-analysis

In vegetated ecosystems, the chemical composition of rainwater is strongly altered after gross rainfall (GR) partitioning into throughfall (TF) and stemflow (SF) before entering the under-canopy soil. However, quantitative syntheses of the chemical alteration are lacking, rendering an insufficient comprehension of its overarching patterns and magnitudes. Here, we furnish a comprehensive dataset related to the chemistry of rainfall partitioning by vegetation in China compiled from 131 peer-reviewed papers published between 1988 and 2023. A meta-analysis was conducted to assess the chemical alterations as GR partitions into TF and SF. We discerned a marked acidification effect in SF but not in TF, with the pH being significantly lower in SF than in GR. Additionally, we observed a persistent concentration-based chemical enrichment of total nitrogen (TN) and total phosphorous (TP) as well as 12 commonly reported nutrient-ions (except for Cu2+) in both TF and SF, with SF generally exhibiting a greater degree of chemical enrichment than TF. Using a machine learning method (boosted regression trees), we identified the key drivers from twelve biotic and abiotic predictor variables on the effect sizes of pH, TN, TP, and each nutrient-ion, and further elucidated the prevalent non-linear partial effects of these key drivers on the magnitude of chemical alterations. For instance, three predictor variables were identified as key drivers for the effect sizes of TN for SF, with a descending relative influence of diameter at breast height (56.9 %; positive effect), Human Footprint (16.5 %; positive), and bark texture (14.0 %; stands with mixed bark textures exerting a greater impact). Our study has significant implications for an accurate estimation of nutrient budgets in vegetated ecosystems throughout China, contributes to a deeper understanding of the intricate ways in which biotic and abiotic predictors influence chemical alterations in rainfall partitioning, and aids in evaluating forest health and developing sensible forest management strategies.

A Study on Effect Analysis and Process Parameter Optimization of Viscous Acid Acidification in a Porous Heterogeneous Carbonate Reservoir

The homogeneous acid etching of conventional acid in porous heterogeneous carbonate reservoirs leads to a large amount of consumption in the near-wellbore area, which makes the acidification effect often not ideal. In order to improve the acidizing effect of porous heterogeneous carbonate reservoirs, viscous acid is used to increase the stimulation of the target block in this paper. Through systematic experiments, the adaptability of the viscous acid in the four layers of the M reservoir in the target block was evaluated, and the MD and ME layers suitable for acidizing stimulation were determined in combination with physical property analysis. Finally, based on the geological characteristics and experimental data of the preferred layers, a two-scale acid wormhole growth radial model was established, and the construction parameters of acidizing stimulation were optimized. The results show that (1) The preferred viscous acid system has a dissolution rate of more than 95% for the rock powder in the four layers. When the matrix permeability is high, the effect of the acid wormhole is obvious and the permeability increase is higher. (2) The steel sheet corrosion and residual acid damage experiments showed that the acid system was not corrosive to the wellbore, and the reservoir damage rate of the residual acid after the reaction was low. (3) Based on the relationship between reservoir porosity and permeability and the position of edge and bottom water, the MD and ME layers with more potential for acidizing stimulation are selected. (4) The results of the numerical simulation show that the optimal acid pump rate of the MD and ME layers is 1.4 bpm and 1.0 bpm, and the acidizing fluid volume is 255 bbl, which can form effective acid wormholes, and the range of reservoir permeability transformation is the largest. The field application results show that the optimization scheme effectively improves the production of oil wells, verifies the practicability of the scheme, and provides a reference for the process optimization of viscous acid in the same type of porous heterogeneous carbonate reservoir stimulation.

Short-term effects of small volume saline infusion on acid base equilibrium in critically ill patients.

Short-term acid-base effects of 0.9% saline solution infusion are not well described. Aim of this study was to assess the effects of a fluid challenge with 0.9% saline in critically ill patients according to the Stewart's approach, which allows a precise determination of acid base equilibrium. In 40 mechanically ventilated critically ill patients, acid-base variables according to Stewart's approach were measured before and after 30 minutes from the infusion of 0.5L of 0.9% saline. Patients were divided in saline responder (fractional sodium excretion increase <0.5) and non-responders, and in patients with (estimated glomerular filtration rate >63 mL/min) and without renal impairment. After saline infusion, plasma sodium concentration did not change (138 [135-141] vs. 138 [135-140] mEq/L, P=0.646), while chloride concentration significantly increased (102 [100-106] vs. 104 [191-106] mEq/L, P=0.003), reducing strong ion difference (37.0 [34.9-38.0] vs. 35.4 [32.7-37.5] mEq/L, P=0.004) without any impact on pH, due to the concomitant albumin dilution. In saline non-responders, the increase of plasma chloride concentration caused a reduction in strong ion difference, while in saline responders both plasma chloride concentration and strong ion difference remained similar. Patients with and without renal impairment presented a similar acid-base response. The infusion of 0.9% saline reduced strong ion difference by increasing plasma chloride concentration, with no effect on pH due to concomitant albumin dilution. Saline non-responders, characterized by the ability to excrete the sodium excess, were more likely to suffer the acidifying effects of saline infusion, while renal function did not affect the acid-base response to saline infusion.

Optimisasi Budidaya Rumput Laut sebagai Benteng Alami untuk Mengurangi Asidifikasi Laut

Anthropogenic carbon dioxide emissions are propelling a concerning rise in ocean acidity, posing severe threats to marine ecosystems, especially calcifying organisms like corals and mollusks. In response to this global challenge, seaweed farming has emerged as a promising remedy. Leveraging their remarkable growth rates and carbon sequestration abilities, seaweeds offer a viable solution to counteract the acidifying effects of elevated carbon dioxide levels in the oceans. Through photosynthesis, seaweeds actively absorb carbon dioxide from seawater, thereby mitigating acidity and fostering improved water quality. The potential of seaweed farms extends beyond mere carbon sequestration. These farms play a pivotal role in habitat creation, absorb nitrogen and phosphorus nutrients, and contribute to enhanced biodiversity. The cultivation of seaweed not only addresses the immediate concern of ocean acidification but also provides a holistic ecological approach with far-reaching benefits. As a sustainable and scalable strategy, seaweed farming exemplifies an innovative and multifaceted solution to the complex challenges posed by anthropogenic impacts on the oceans, underlining the importance of nature-based interventions in preserving the health and balance of marine ecosystems.

Migration and morphological transformation patterns of heavy metals on sludge cells and extracellular polymeric substances (EPS) under the influence of different treatments.

The impediment of sludge resource utilization stems from the presence of heavy metals within the sludge matrix. To optimize heavy metal removal techniques from undried sludge, it is essential to study the distribution of heavy metals in the sludge flocs structure and the changes in morphology in the sludge cells after different treatments. In this study, the sludge was subjected to chemical treatments using citric acid (CA), EDTA, and saponin, as well as electrokinetic treatment at 2 V/cm. The distribution and migration of Cu, Ni, and Zn in sludge flocs after various treatment methods were analyzed. The heavy metals were found to migrate from intracellular to extracellular polymeric substances (EPS) without causing extensive sludge cell lysis. They gradually diffused outward with the dispersion of the EPS layer. The migration efficiency of the three heavy metals in the sludge flocs was Zn, Ni, and Cu. This was mainly related to the initial distribution and morphology of the heavy metals. Under the influence of chemicals and an electric field, the acid-soluble and reducible heavy metals in the cells partially migrated to the EPS, while the stable heavy metals transformed into an unstable state. Furthermore, the order of chemical reagents in terms of their effect on the migration efficiency of heavy metals was CA > EDTA > Saponin, owing to the varying binding strengths of heavy metals and their impact on the degree of loosening of the EPS. Especially after CA treatment a greater proportion of Cu, Ni, and Zn were transferred from the cells to the EPS. The acidification effect near the anode during electrokinetic treatment intensifies the migration of heavy metals. This study provides basic research for subsequent engineering optimization aimed at removing heavy metals from sludge.

Effect of probiotics and acidifiers on feed intake, egg mass, production performance, and egg yolk chemical composition in late-laying quails.

Probiotics can be used as an alternative to antibiotic growth promoters because antibiotics are prohibited worldwide. This study investigated the potential combination of probiotics and acidifiers to improve feed intake, productive performance, egg mass, and egg yolk chemical composition of late-laying quail for the health of humans who consume quail products. One hundred laying quails were divided into 4 × 5 treatments, with each group consisting of five replications. The adaptation period was 2 weeks, and the treatment was continued for 4 weeks. Probiotics and acidifiers were added to drinking water and incorporated into the diet. Feed and water were provided ad libitum. Treatment duration (1 week, 2 weeks, 3 weeks, and 4 weeks) and additional feed treatment (control, probiotic 2% + 0.5% acidifier, probiotic 2% + 1% acidifier, probiotic 4% + 0.5% acidifier, and probiotic 4% + 1% acidifier, respectively). Significant differences (p < 0.05) were observed in feed intake, quail day production, feed efficiency, egg mass in laying quails, and the chemical composition of egg yolk with probiotics and acidifiers in late-laying quails. The combination of probiotics and acidifiers can improve feed intake, production performance, egg mass, and egg yolk chemical composition in late-laying quails.

Pore Evolution Law and Gas Migration Characteristics of Acidified Anthracite in Liquid CO2‐ECBM: An Experimental Study

The high gas pressure, low permeability, and strong gas absorption of coal seams in China complicate gas extraction, severely restricting the efficient development of coalbed gas. Liquid CO2 (LCO2) has a dual effect of cracking and enhancing the permeability of coal rock, thus, enhancing gas recovery. In this study, experimental testing and comparative analyses were performed to analyze the LCO2 acidification of antireflection coal, for which reference and variable experimental groups were designed. The acidification effect was quantitatively analyzed by examining changes in the pH value of the reaction solution, mineral content of coal, and pore structure during the experimental process. The experimental results indicated that a higher pressure resulted in a greater amount of CO2 being dissolved and a stronger acidity of the reaction water sample. As the reaction time increased, the minerals in the coal gradually dissolved and more H+ ions in the solution were consumed. The calcite (carbonate) and illite (clay mineral) contents significantly decreased, which is the main reason for the change of coal pore structure. The minerals on the pore surface of coal react with (CO2–H2O) weak acid, which increases the development of pore branches, improves the complexity of coal pores, and roughens the pore surface. Acidification significantly increases the number of micropores (&lt;10 nm) and small pores (10–100 nm) in coal; furthermore, pore development is simple, the surface is smooth, and the pore connectivity is improved, which makes this part of pores have fractal characteristics. CO2–H2O–coal acidification increases the pore volume corresponding to the CO2/CH4 gas slippage flow in coal and strengthens the mass and energy transfer of CO2/CH4 in coal. CO2 can enter more pores than CH4 and displace the adsorbed and free CH4 in the pores.

Research on high efficiency and retarded autogenous mud acid for plugging removal in a sandstone reservoir—Example of Bai 823 well area

AbstractAcidification, as an effective measure to remove blockages and restore oil well productivity, has received widespread concern. However, conventional acid systems can only be used to unblock near‐wellbore zones and are difficult to act on far‐wellbore reservoirs. In this article, scanning electron microscope, energy‐dispersive X‐ray spectroscope, and X‐ray diffractometer were used to analyze the blockages in the Bai 823 well area, and it was determined that their main components were CaCO3 and FeCO3, which provided the basis for the construction of the unblocking formula. Then, the acid dissolution experiments on the plugging materials showed that the dissolution rate of 10% HCl can reach more than 85% for the scale sample; the acid dissolution experiments on the rock sample showed that the autogenous mud acid has better dissolution performance and better retardation performance for the rock sample, and the effective action time is longer, so it can be used for deeper unplugging of the reservoir. Finally, the process parameters such as the optimal acid concentration, acid dosage, and discharge volume were optimized by establishing the fracture‐matrix acid flow reaction model. Research results indicate that the autogenous mud acid can act on the blockage in the far‐wellbore area to improve the acidification effect.

Biochar and Silicon for Sustainable Agriculture in Acid Soil- Nutrient Dynamics and Maize Production: A Review

The central part of the country in the world classified Maize as their staple and essential crop, but this crop cannot cultivate appropriately in acidic soil. Thus, biochar, silicon and phosphorus observation can act as an agent to improve the growth of Maize in acidic soil. Additionally, biochar, well known for its alkaline properties, can reduce soil acidification and, at the same time, improve crop production. This low pH condition was caused by aluminium, manganese and low of phosphorus. Thus, silicon and Phosphorus can improve crop productivity. Using biochar as a soil amendment raises the pH value, alone or in combination. Maize (Zea mays L.) and the acidity of the soil are much related to the application of biochar combined with silicon and Phosphorus. Biochar simply can reduce exchangeable soil acidity, but when combined with silicon and Phosphorus, it can have a greater influence on reducing soil Al toxicity. The importance of biochar with different rates combined with silicon and Phosphorus to increase the pH of soil is still an inconsistent result by various studies. This review summarizes the properties of biochar, silicon and phosphorus and provides the scientific reference for its application to archive high yield of Maize and reduce the acidification effect on soil.

Effects of antibiotic, acidifier, and probiotic supplementation on mortality rates, lipoprotein profile, and carcass traits of broiler chickens

Antimicrobial resistance is a significant issue, therefore it's relevant to assess the effects of antibiotics, acidifiers, and probiotic supplementation finding a good alternative to reduce the use of antibiotics in broiler production in rural areas of Bangladesh. Using randomized control trial, this 28-day study evaluated 360 Hubbard Classic broiler chicks divided into four groups: oxytetracycline-treated, acidifier-treated, Lactobacillus-based probiotic-treated, and control (no antibiotics, acidifiers, or probiotics). Each group was replicated three times with 30 birds each with adlibitum feeding. Body weight and feed intake were recorded weekly, and on 28th day, carcass traits and blood lipoprotein levels were evaluated. Results showed that in first and fourth weeks, the body weight gain significantly varied in probiotics and acidifier-treated birds than the control group (P < 0.001). The probiotic group had gained considerable increase in body weight (185.0 g vs 161.7 g and 1745.0 g vs 1592.7 g) than the control group. Notably, in the first week, the feed conversion ratio for the probiotic group was 0.76, but the antibiotic group's was 0.96 (P < 0.001). The weights of the drumstick (88.33 g) and liver (61.0 g) having probiotic supplements were substantially higher than those in the control group (77.0 g and 51.33 g, respectively) (P < 0.001). According to serum lipoprotein analysis, the probiotic and acidifier groups exhibited lower LDL levels (71.1 mg/dl and 69.8 mg/dl, respectively) and higher triglyceride levels (122.9 mg/dl and 135.4 mg/dl). These findings highlight the potential of probiotics and acidifiers as effective antibiotic alternatives, promoting carcass traits and lowering LDL levels in broilers in Bangladesh.

A Review on Lactic Acidosis in Cardiac Surgery

The normal range of lactate is 0-2mmol/L and value above it is considered as hyperlactatemia. It may arise due to adequate and non-adequate oxygen supply mechanisms. The major reason is likely stress–induced aerobic mechanism which shows raised results of lactate. Increased lactate levels can cause acidifying effects on the blood. There are many etiological reasons for lactic acidosis like disease condition, deficiency and alcohol. Early and late onset are two categorizations for patient with hyperlactatemia after cardiac surgery. On being the major trigger factor for lactic acidosis in cardiopulmonary surgery patients due to tissue hypoperfusion. So preventive measures before cardiac surgery should be taken.

Lactulose in cirrhosis: Current understanding of efficacy, mechanism, and practical considerations.

HE is a complication of cirrhosis characterized by neuropsychiatric and motor dysfunction, and results in decreased quality of life and increased mortality. Lactulose is a synthetic disaccharide used to treat HE since 1966, though many questions about its use remain unanswered. Lactulose reverses minimal HE, prevents overt HE, improves quality of life, increases the rate of recovery from overt HE, and improves survival rates. Lactulose's clinical effect appears to be derived from its impact on intestinal microbes, likely a result of its enteric acidifying effect, positive pressure on beneficial taxa, and improvement of gut barrier function. There are several practical considerations with lactulose including (1) a need to avoid excessive bowel movements and subsequent dehydration, (2) treatment titration protocols need further investigation, (3) baseline or treatment-induced gastrointestinal side effects limit adherence in some cases, and (4) the utility of monitoring stool consistency or pH remains unknown. Further research is needed to optimize our use of this effective treatment for HE.

Использование подкислителей и бутиратов в составе рационов – путь к реализации биоресурсного потенциала цыплят-бройлеров

Abstract. In the conditions of the research and production laboratory of the ERC “Agrotechnopark” of the Belgorod State Agrarian University, a scientific and economic experiment was carried out to study the effect of feed additives acidifier “AsidLak” and “ButiPERL” calcium butyrate on slaughter indicators, morphology of broiler carcasses, chemical composition of pectoral and leg muscles, organoleptics of meat and broth. Methods. Chickens of the 1st experimental group were introduced into the main diet of the acidifier “AsidLak” in the amount of 5 kg/t, the 2nd experimental group was injected with 0.3 kg/t of calcium butyrate “ButiPERL”, the 3rd experimental group – a complex of feed additives in dosages 1 and 2 of the experimental groups without the introduction of antimicrobial drugs; broiler chickens of the control group received a complete mixed feed (OP). When growing chickens, a three-phase feeding system was used. During the experimental period, broiler chickens were grown outdoors, under the same conditions. According to the results of the study, it was found that broilers of the 3rd experimental group had the highest pre-slaughter live weight in comparison with the control by 104.67 g or 4.0% (P ≥ 0.95), which is also higher than analogues 1 and 2 experimental by 14 g (0.51%) and 48 g (1.76 %); broilers of the 3rd experimental group had a greater mass of gutted carcass – 1870.73 g, which is higher compared to the control group by 132.96 (7.65 %), and also 1 and 2 experimental by 43.93 g (2.35 %) and 77.96 g (4.17 %). 68.7 %. An increase in the content of dry matter, fat and protein in the pectoral and leg muscles, as well as an improvement in the taste and biological value of meat was noted. The scientific novelty lies in the fact that for the first time on the livestock of the Ross-308 cross, an acidifier and butyrate were used in combination as an alternative to antimicrobial drugs.

Long-term risk assessment of uprooting and stem breakage under drought conditions and at high N deposition in beech and Norway spruce

Damage by wind in forests has increased in recent decades, counteracting possible growth increases by nitrogen (N) deposition. This is also shown by the long-term forest monitoring data from this study. The main factors affecting uprooting and stem breakage in Fagus sylvatica (L.) (9′846 individual beech trees in 102 plots) and Picea abies (Karst.) (9′168 Norway spruce trees in 72 plots) have been analysed by binomial mixed regression models. Our long-term forest observations showed that drought increased uprooting of beech and increased both uprooting and stem breakage of Norway spruce. In beech, high N deposition interacted with drought, resulting in increased uprooting under drought. In Norway spruce, N deposition increased stem breakage directly. Foliar potassium (K) deficiency increased uprooting of both beech and Norway spruce. Moreover, we found an indirect effect of soil acidification in increasing uprooting of beech by decreasing foliar calcium (Ca) concentrations. In Norway spruce, the soil acidification effect was confounded by foliar K concentrations. In beech, stem breakage increased with higher temperature which can be explained by a temperature-mediated growth increase of white rot fungi. In contrast, temperature was negatively related with stem breakage in Norway spruce, which may be interpreted as an inhibition of the heartrot fungus Heterobasidion at higher temperatures.In summary, drought and high N deposition increase the risk of mechanical damage to both beech and Norway spruce and thus endanger sustainable growth rates. We therefore suggest that wind damage must be taken in account in productivity calculations and carbon budgets of temperate forests.

Responses of soil microbial communities and enzyme activities under nitrogen addition in fluvo-aquic and black soil of North China.

This research investigates the impact of long-term nitrogen (N) addition on fluvo-aquic and black soils in north China, with a focus on soil microbial communities and enzyme activities. In each site, there were three N fertilization treatments, i.e., control, moderate-N, and high-N. Phospholipid Fatty Acid Analysis was employed to analyze the microbial community composition, and enzyme activities related to N, carbon (C), and phosphorus (P) cycling were assessed. The results showed that increasing N fertilization levels led to higher soil organic carbon (SOC) and total N (TN) concentrations, indicating enhanced nutrient availability. N fertilization reduced soil pH across both soils, with a more pronounced acidification effect observed in the black soil. Across both soils, N addition increased maize yield, but the higher crop yield was attained in moderate-N rate compared with high-N rate. Microbial community composition analysis revealed that N fertilization induced shifts in the relative abundances of specific microbial groups. The black soil exhibited pronounced shifts in the microbial groups compared to the fluvo-aquic soil, i.e., decreased fungal abundance and fungi: bacteria ratio in response to N input. In addition, the application of N fertilizer led to an elevated ratio of gram-positive to gram-negative (GP:GN) bacteria, but this effect was observed only in black soil. N fertilization had an impact on the enzyme activities related to C, N, and P cycling in both soil types, but black soil showed more pronounced changes in enzyme activities. Permutational multivariate analysis of variance indicated that soil types rather than N fertilization mediated the response of the soil microbial community and enzyme activities. Partial least square path modeling demonstrated that soil pH was the only key driver impacting soil microbial groups and enzyme activities in both soils. In conclusion, our findings highlighted that N fertilization exerted more pronounced impacts on soil biochemical properties, microbial community composition, and enzyme activities in black soil furthermore, moderate N rate resulted in higher crop productivity over high N rate.

NaCl-mediated strategies for the trade-off between Cd bioconcentration and translocation in Solanum nigrum L

Salt interference significantly affects the behavior of heavy metals in the environment. This study compared and analyzed the response process, migration, and transformation of cadmium (Cd) in the hyperaccumulator Solanum nigrum (S. nigrum) under different NaCl levels to reveal the interference mechanisms of salt in plant remediation of Cd-contaminated soil. The results showed that Cd and salt stress significantly inhibited the growth of plants. The stress effect had more potent growth inhibition at the root than aboveground, thus inducing changes in the spatial configuration of the plants (decreased root-to-aboveground biomass ratio). Salt could activate Cd in plants, enhancing the inhibitory effect on plant growth. Salt increased Cd bioavailability due to the rhizosphere acidification effect, increasing plants’ Cd accumulation. The Cd bioconcentration factor in plant roots peaked during the high Cd-high salt treatment (117.10), but the Cd accumulation of plants peaked during the high Cd-low salt treatment (233.04 μg plant−1). Salt additions and increased Cd concentrations enhanced root compartmentalization, inhibiting Cd transport to the aboveground. Changes in Fourier-transform infrared spectroscopy (FTIR) measurements confirmed that the functional groups in plants provided binding sites for Cd. These findings can help guide the phytoremediation of Cd contamination under saline soil conditions.