Many reports suggest that exogenous Si addition could reduce Cd translocation to aerial part and make grain safe for rice production. But it remains unclear whether its supplementation during different growth phases can differentially impact Cd uptake in rice. Here, Positive effects of Si applied at different growth phases on biomass and yield of rice were observed. Dry weight of shoot including stem, leaf and grain was enhanced significantly by 15% when Si added at transplanting stage. Grain-yields supplied with Si at transplanting, jointing and heading stages were increased obviously by 14%, 11% and 12%, respectively. Higher percentage of filled spikelet and lower unfilled spikelet number per panicle were found when Si supplied at jointing stage. The increases of Cd accumulation in rice plants by Si application were mainly manifested in roots. Compared to CK, Cd accumulation in root when Si applied at transplanting and tillering stages were elevated by 75% and 64%, respectively. While Cd accumulation in aboveground tissues were all declined by Si addition. Bioconcentration and translocation factors were decreased significantly when Si added at jointing stage. In addition, higher soil pH, lowest available Cd-concentration in soil and Cd2+ concentration in xylem sap at 15d after flowering were found when the Si was applied at jointing stage. Overall, Si application at transplanting would be more beneficial to the growth of rice and increased Cd content in root, while Si supplied at jointing would be more favorable for grain filling and reducing Cd accumulation in shoot.