Accumulation In Different Tissues Research Articles

Dynamic interplay of silica-coated iron oxide nanocomposite on soil-plant system to mitigate Cd toxicity in rice

The utilization of nanotechnology based ameliorative strategies has emerged as an effective method to reduce the potential toxicity of environmental stressors on crops. However, there is limited information available regarding the interaction of silica-coated iron oxide nanocomposite (Fe3O4@SiO2, NCs) with plants under abiotic stress. Therefore, the objective of this study was to investigate the effects of different doses of silica-coated iron oxide nanocomposite (NCs) (25/50 mg Kg-1) in mitigating Cd phytotoxicity in rice. The results revealed that Cd-contaminated soil (30 mg Kg-1) inhibited plant growth, reduced physiological attributes, and induced ultrastructural changes in stressed plants. The presence of Cd in the soil also reduced rhizospheric soil pH and soil enzyme activities while increased Cd bioavailability and its uptake in plants. However, the foliar application of silica coated iron oxide NCs stimulated plant biomass production, chlorophyll biosynthesis, and antioxidant capacity. The application of NCs significantly decreased the activities of lipid peroxidation markers (MDA, EL and LOX) and reduced levels of reactive oxygen species (ROS) accumulation (•OH, O2·− and H2O2) in stressed plants. Additionally, NCs application influenced Cd accumulation in different tissues, potentially through modifying chemical profile of root exudates (amino/organic acids), which facilitated Cd precipitation through chelation and its sequestration in vacuoles via modulating the expression of metal transporters. Furthermore, the application of NCs indirectly regulated Cd fractionation, solubility, mobility and Cd enrichment in the rhizosphere. This study demonstrates the effective mitigation of Cd toxicity in rice plants by applying silica-coated iron oxide NCs. The results provide valuable insights into nanotechnology-based approaches for improving plant stress tolerance and offer promising strategies for sustainable agricultural practices.

Comparative transcriptome analysis reveals transcriptional regulation of anthocyanin biosynthesis in purple radish (Raphanus sativus L.)

Radish exhibits significant variation in color, particularly in sprouts, leaves, petals, fleshy roots, and other tissues, displaying a range of hues such as green, white, red, purple, and black. Although extensive research has been conducted on the color variation of radish, the underlying mechanism behind the variation in radish flower color remains unclear. To date, there is a lack of comprehensive research investigating the variation mechanism of radish sprouts, leaves, fleshy roots, and flower organs. This study aims to address this gap by utilizing transcriptome sequencing to acquire transcriptome data for white and purple radish flowers. Additionally, the published transcriptome data of sprouts, leaves, and fleshy roots were incorporated to conduct a systematic analysis of the regulatory mechanisms underlying anthocyanin biosynthesis in these four radish tissues. The comparative transcriptome analysis revealed differential expression of the anthocyanin biosynthetic pathway genes DFR, UGT78D2, TT12 and CPC in the four radish tissues. Additionally, the WGCNA results identified RsDFR.9c and RsUGT78D2.2c as hub genes responsible for regulating anthocyanin biosynthesis. By integrating the findings from the comparative transcriptome analysis, WGCNA, and anthocyanin biosynthetic pathway-related gene expression patterns, it is hypothesized that genes RsDFR.9c and RsUGT78D2.2c may serve as pivotal regulators of anthocyanins in the four radish tissues. Furthermore, the tissue-specific expression of the four copies of RsPAP1 is deemed crucial in governing anthocyanin synthesis and accumulation. Our results provide new insights into the molecular mechanism of anthocyanin biosynthesis and accumulation in different tissues of radish.

A Fruit-Expressed MYB Transcription Factor Regulates Anthocyanin Biosynthesis in Atropa belladonna.

Anthocyanins are water-soluble flavonoid pigments that play a crucial role in plant growth and metabolism. They serve as attractants for animals by providing plants with red, blue, and purple pigments, facilitating pollination and seed dispersal. The fruits of solanaceous plants, tomato (Solanum lycopersicum) and eggplant (Solanum melongena), primarily accumulate anthocyanins in the fruit peels, while the ripe fruits of Atropa belladonna (Ab) have a dark purple flesh due to anthocyanin accumulation. In this study, an R2R3-MYB transcription factor (TF), AbMYB1, was identified through association analysis of gene expression and anthocyanin accumulation in different tissues of A. belladonna. Its role in regulating anthocyanin biosynthesis was investigated through gene overexpression and RNA interference (RNAi). Overexpression of AbMYB1 significantly enhanced the expression of anthocyanin biosynthesis genes, such as AbF3H, AbF3'5'H, AbDFR, AbANS, and Ab3GT, leading to increased anthocyanin production. Conversely, RNAi-mediated suppression of AbMYB1 resulted in decreased expression of most anthocyanin biosynthesis genes, as well as reduced anthocyanin contents in A. belladonna. Overall, AbMYB1 was identified as a fruit-expressed R2R3-MYB TF that positively regulated anthocyanin biosynthesis in A. belladonna. This study provides valuable insights into the regulation of anthocyanin biosynthesis in Solanaceae plants, laying the foundation for understanding anthocyanin accumulation especially in the whole fruits of solanaceous plants.

Assessment of mercury accumulation in various tissues of rice plant (Oryza sativa L.) from a Ramsar site in India

Dietary exposure to mercury through rice consumption is an issue of solemn global concern. The favourable bio-geochemical environment of paddy fields often makes them potential mercury cycling hotspots. India is one of the largest producers and consumers of rice globally. To date, studies focusing on mercury bioaccumulation in rice during the different stages of its growth cycle have not been studied in Indian agro-ecosystems.This inturn resulted in a knowledge gap in the global mercury assessment. Being a party to the Minamata Convention, there is an urgent need to unravel the scenario of mercury contamination in rice, one of the staple food crops across the world. In the present study, total mercury (THg) in the rhizosphere soil as well as its accumulation in different tissues of the rice plant were examined by a field investigation in Kuttanad – a unique Ramsar site in Kerala, India. For the purpose of the study five permanent plots were selected and monitored for the entire rice-growing season (from the 30th to the 120th day). Direct Mercury Analyzer (DMA – 80, Milestone, USA) was used for analyzing THg in rhizosphere soil and plant tissues. Mercury concentration in soils varied from 0.037 mg/kg to 0.117 mg/kg with a mean concentration of 0.088 mg/kg. The THg accumulation pattern in plant tissues followed the order: root > leaf > grain > husk > stem. The mean THg concentration in rice grains was 0.22 mg/kg. The low values for bioaccumulation factor (0.616) and translocation factor (0.425) revealed that a major portion of mercury accumulated by the plant is stored in the roots.

Systematic analysis of the UDP-glucosyltransferase family: discovery of a member involved in rutin biosynthesis in Solanum melongena.

Eggplant (Solanum melongena) is an economically important crop and rich in various nutrients, among which rutin that has positive effects on human health is found in eggplant. Glycosylation mediated by UDP-glycosyltransferases (UGTs) is a key step in rutin biosynthesis. However, the UGT gene has not been reported in eggplant to date. Herein, 195 putative UGT genes were identified in eggplant by genome-wide analysis, and they were divided into 17 subgroups (Group A-P and Group R) according to the phylogenetic evolutionary tree. The members of Groups A, B, D, E and L were related to flavonol biosynthesis, and rutin was the typical flavonol. The expression profile showed that the transcriptional levels of SmUGT genes in Clusters 7-10 were closely related to those of rutin biosynthetic pathway genes. Notably, SmUGT89B2 was classified into Cluster 7 and Group B; its expression was consistent with rutin accumulation in different tissues and different leaf stages of eggplant. SmUGT89B2 was located in the nucleus and cell membrane. Virus-induced gene silencing (VIGS) and transient overexpression assays showed that SmUGT89B2 can promote rutin accumulation in eggplant. These findings provide new insights into the UGT genes in eggplant, indicating that SmUGT89B2 is likely to encode the final enzyme in rutin biosynthesis.

Effects of chronic exposure of cadmium on growth performance, bioaccumulation and hematological parameters of genetically improved farmed tilapia

This study was conducted to evaluate the chronic exposure of cadmium on growth performance, bioaccumulation, and hematological parameters of genetically improved farmed tilapia (GIFT). Two concentrations of cadmium (50 μgL-1 and 100 μgL-1) with control were used. Twelve fish with an initial mean weight of 12.84 ± 0.53 g were randomly assigned quadruplicated treatments (total 144 fish) and the experiment lasted for six weeks. In the end, growth performance and feed utilization efficiencies were assessed by using % specific growth rate (%SGR), % average daily gain (%ADG) and feed conversion ratio. The pattern of Cd accumulation in different tissues and hematological parameters were examined. Mean body weight, %ADG, %SGR, hematocrit and packed cell volume were not affected by the treatments. A significantly higher survival rate (100 %) was observed in control followed by CD50 (87.50 %) and CD100 (78.13 %). The rate of Cd accumulation in various tissues was in the order of liver > gills > muscle > skin in each treatment. Among tested concentrations, the highest accumulation was observed in CD100 for each tissue. Both red blood and white blood cell count (2.21 ± 0.07 × 106 mm-3 and 468.00 ± 6.61 × 103 mm-3 respectively) were significantly higher in control and it was lowest in CD 100 (1.28 ± 0.04 × 106 mm-3 and 205.19 ± 8.94 × 103 mm-3). The results of this study supported the conclusion that there is a significant effect of the chronic exposure to cadmium on the bioaccumulation and hematological parameters of the GIFT tilapia even at the ecologically relevant concentration.

A MYB activator, DcMYB11c, regulates carrot anthocyanins accumulation in petiole but not taproot.

The first domesticated carrots were thought to be purple carrots rich in anthocyanins. The anthocyanins biosynthesis in solid purple carrot taproot was regulated by DcMYB7 within P3 region containing a gene cluster of six DcMYBs. Here, we described a MYB gene within the same region, DcMYB11c, which was highly expressed in the purple pigmented petioles. Overexpression of DcMYB11c in 'Kurodagosun' (KRDG , orange taproot carrot with green petioles) and 'Qitouhuang' (QTHG , yellow taproot carrot with green petioles) resulted in deep purple phenotype in the whole carrot plants indicating anthocyanins accumulation. Knockout of DcMYB11c in 'Deep Purple' (DPPP , purple taproot carrot with purple petioles) through CRISPR/Cas9-based genome editing resulted in pale purple phenotype due to the dramatic decrease of anthocyanins content. DcMYB11c could induce the expression of DcbHLH3 and anthocyanins biosynthesis genes to jointly promote anthocyanins biosynthesis. Yeast one-hybrid assay (Y1H) and dual-luciferase reporter assay (LUC) revealed that DcMYB11c bound to the promoters of DcUCGXT1 and DcSAT1 and directly activated the expression of DcUCGXT1 and DcSAT1 responsible for anthocyanins glycosylation and acylation, respectively. Three transposons were present in the carrot cultivars with purple petioles but not in the carrot cultivars with green petioles. We revealed the core factor, DcMYB11c, involved in anthocyanins pigmentation in carrot purple petioles. This study provides new insights into precise regulation mechanism underlying anthocyanins biosynthesis in carrot. The orchestrated regulation mechanism in carrot might be conserved across the plant kingdom and useful for other researchers working on anthocyanins accumulation in different tissues.

Accumulation of polystyrene nanoplastics and triclosan by a model tooth-carp fish, Aphaniops hormuzensis (Teleostei: Aphaniidae)

The presence and effects of nanoplastics (NPs; <1 μm) in the aquatic environment are a growing concern. In this study, a model tooth-carp fish, Aphaniops hormuzensis, has been exposed to different concentrations of fluorescent polystyrene nanoplastics (PS-NP) in its diet (up to 5 mg kg−1) over periods of 28 d and the particle accumulation in different tissues determined. Accumulation was observed in both digestive and non-digestive organs, with concentrations greater in the gut, liver and gill (up to 400 μg kg−1 dw) than in the skin and muscle (<180 μg kg−1 dw), but no dependency on exposure time or dose was evident. The presence of the organic contaminant, triclosan (TCS), in the diet and at concentrations up to 0.5 μg kg−1 did not affect PS-NP uptake by A. hormuzensis, while TCS accumulation in the whole body increased with time (up to 10 μg kg−1) and, likewise, appeared to be unaffected by the presence of PS-NPs. These observations suggest that the two contaminants do not interact with each other or that any interactions have no impact on accumulation. The results of this study add to the growing body of evidence that NPs can be translocated by aquatic organisms after ingestion, and reveal that, for the species and conditions employed, nanoplastics are accumulated more readily than a widely used organic chemical.

Nonalcoholic Fatty Liver Disease Severity Is Related to Plasma Pro-Oxidative Biomarkers Rather Than Liver Tissue-Measured Nitrogen Metabolism Biomarkers in Population with Obesity and Metabolic Syndrome.

Background: The metabolic syndrome (MS) is associated with an increased production of nitrogen metabolites and elevated oxidative stress, which favors progression of nonalcoholic fatty liver disease (NAFLD). Subjects with the phenotype known as metabolically unhealthy obese (MUO) meet most of the MS cardiometabolic risk criteria and show a higher risk of advanced NAFLD severity, compared with the so-widely known metabolically healthy obese (MHO). Obese individuals with MS are more susceptible to abnormal lipid accumulation in different tissues, whereas oxidative stress and nitrogen metabolites are increased in MS and/or obesity. This study aimed to explore whether plasma- or liver tissue-determined biomarkers of nitrogen metabolism and oxidative stress relate to NAFLD severity and/or metabolic phenotype. Methods: This cross-sectional study included candidates for bariatric surgery with biopsy-proven NAFLD diagnosis and staging. For comparison, the study population was divided according to NAFLD damage (steatohepatitis F0-F1 vs. steatohepatitis F2-F4) and metabolic phenotype (MHO vs MUO, based on the MS criteria). Hepatic and plasma concentrations of nitrogen metabolites and oxidative stress biomarkers were determined by enzymatic kinetics assays, enzyme-linked immunosorbent assay, and Greiss reaction. Results: The study population (N = 45) was constituted by patients with obesity and higher prevalence of dyslipidemia, diabetes mellitus, and hypertension. According to plasma biomarkers, MUO phenotype was related to higher cardiometabolic risk; meanwhile, advanced NAFLD damage was related to higher glycated hemoglobin (HbA1c) and triglycerides. Elevated hepatic concentrations of ammonium, nitrites, arginine, and citrulline were found in MUO phenotype, but only higher plasma concentration of malondialdehyde was found as specifically related to advanced NAFLD damage. Conclusions: Circulating biomarkers of redox state were selectively related to advanced NAFLD damage, suggesting prognostic and therapeutic targets. Hepatic concentrations of nitrogen metabolism biomarkers may be more related to cardiometabolic risk.

SREKA-targeted liposomes for highly metastatic breast cancer therapy

Chemotherapy is still a leading therapeutic approach in various tumor types that is often accompanied by a poor prognosis because of metastases. PEGylated liposomes with CREKA targeting moiety are well-known therapeutic agents, especially in highly metastatic experimental models. CREKA specifically targets tumor-associated ECM, which is present at the primary, as well as metastatic tumor sites. To better understand the function of the targeting moieties, we decided to design various liposome formulations with different amounts of targeting moiety attached to their DSPE-PEG molecules. Moreover, a new tumor-homing pentapeptide (SREKA) was designed, and a novel conjugation strategy between SREKA and DSPE-PEGs. First, the in vitro proliferation inhibition of drug-loaded liposomes and the cellular uptake of their cargo were investigated. Afterward, liposome stability in murine blood and drug accumulation in different tissues were measured. Furthermore, in vivo tumor growth, and metastasis inhibition potencies of the different liposome formulations were examined. According to our comparative studies, SREKA-liposomes have a uniform phenotype after formulation and have similar characteristics and tumor-homing capabilities to CREKA-liposomes. However, the exchange of the N-terminal cysteine to serine during conjugation results in a higher production yield and better stability upon conjugation to DSPE-PEGs. We also showed that SREKA-liposomes have significant inhibition on primary tumor growth and metastasis incidence; furthermore, increase the survival rate of tumor-bearing mice. Besides, we provide evidence that the amount of targeting moiety attached to DSPE-PEGs is largely responsible for the stability of liposomes, therefore it plays an important role in toxicity and targeting.

Cooking methods effectively alter perfluoroalkyl substances and nutrients in cultured and wild bullfrogs

The ubiquitous distribution of perfluoroalkyl substances (PFASs) poses a threat to the health of aquatic organisms and humans. Bullfrogs are considered a popular aquatic food product in South China, providing high protein and tasty cuisine; however bullfrogs have been shown to contain significant concentrations of PFASs. However, the risk-benefit ratios of PFASs and nutrient contents in cooked bullfrogs are not well understood. PFASs and nutrients were investigated in raw and cooked specimens of cultured and wild bullfrogs in this study. Novel PFASs showed higher detection levels and accumulation in wild bullfrogs than in cultured bullfrogs. Potential factors such as fat and fatty acid ratio affected PFASs accumulation in different tissues and by different cooking methods of bullfrogs. All cooking methods can reduce PFASs in edible tissues while significantly enhancing the nutritive value index (NVI) compared to raw bullfrogs. Steaming was the most effective way to reduce PFASs (rate of reduction was over 66%) and resulted in a lower risk of contributing to arteriosclerosis than other cooking methods assessed by atherogenicity index (AI) values. Cultured bullfrogs instead of wild bullfrogs were recommended for human consumption, and steaming was regarded as a better cooking method in terms of risk-benefit concerns. Overall, this work provides quantitative analysis of cooking methods that alter PFASs and nutrients in bullfrogs.

Identification of key gene networks controlling polysaccharide accumulation in different tissues of Polygonatum cyrtonema Hua by integrating metabolic phenotypes and gene expression profiles.

Plant polysaccharides, a type of important bioactive compound, are involved in multiple plant defense mechanisms, and in particular polysaccharide-alleviated abiotic stress has been well studied. Polygonatum cyrtonema Hua (P. cyrtonema Hua) is a medicinal and edible perennial plant that is used in traditional Chinese medicine and is rich in polysaccharides. Previous studies suggested that sucrose might act as a precursor for polysaccharide biosynthesis. However, the role of sucrose metabolism and transport in mediating polysaccharide biosynthesis remains largely unknown in P. cyrtonema Hua. In this study, we investigated the contents of polysaccharides, sucrose, glucose, and fructose in the rhizome, stem, leaf, and flower tissues of P. cyrtonema Hua, and systemically identified the genes associated with the sucrose metabolism and transport and polysaccharide biosynthesis pathways. Our results showed that polysaccharides were mainly accumulated in rhizomes, leaves, and flowers. Besides, there was a positive correlation between sucrose and polysaccharide content, and a negative correlation between glucose and polysaccharide content in rhizome, stem, leaf, and flower tissues. Then, the transcriptomic analyses of different tissues were performed, and differentially expressed genes related to sucrose metabolism and transport, polysaccharide biosynthesis, and transcription factors were identified. The analyses of the gene expression patterns provided novel regulatory networks for the molecular basis of high accumulation of polysaccharides, especially in the rhizome tissue. Furthermore, our findings explored that polysaccharide accumulation was highly correlated with the expression levels of SUS, INV, SWEET, and PLST, which are mediated by bHLH, bZIP, ERF, ARF, C2H2, and other genes in different tissues of P. cyrtonema Hua. Herein, this study contributes to a comprehensive understanding of the transcriptional regulation of polysaccharide accumulation and provides information regarding valuable genes involved in the tolerance to abiotic stresses in P. cyrtonema Hua.

Antimony accumulation in zebrafish (Danio rerio) and its effect on genotoxicity, histopathology, and ultrastructure

Antimony (Sb) is a toxic metal in aquatic ecosystems. In this study, the accumulation of aqueous Sb in the liver, brain, gills and muscle of zebrafish (Danio rerio) and its effect on genotoxicity, histopathology and ultrastructure alterations were evaluated. The fishes were exposed to different concentrations (0, 8.29, 16.58, 33.16 mg L−1) of aqueous Sb for 18 days. The results showed that the order of Sb accumulation in different tissues was liver > gill > muscle > brain, and the accumulation increased with increasing Sb stress concentration. The mRNA expression levels of Nrf2, Cu/Zn-SOD, Mn-SOD, CAT and GPx genes showed different trends. In addition, significant histopathology and ultrastructure alterations were observed in the liver and gills exposed to Sb. Sb could accumulate in different tissues of zebrafish, inducing the expression of oxidative stress genes and activating antioxidant defense systems. Histological and ultrastructural changes could be used as valid biomarkers for the assessment of aqueous Sb contamination.

Neuronopathic Gaucher disease: Beyond lysosomal dysfunction.

Gaucher disease (GD) is an inherited disorder caused by recessive mutations in the GBA1 gene that encodes the lysosomal enzyme β-glucocerebrosidase (β-GC). β-GC hydrolyzes glucosylceramide (GluCer) into glucose and ceramide in the lysosome, and the loss of its activity leads to GluCer accumulation in different tissues. In severe cases, enzymatic deficiency triggers inflammation, organomegaly, bone disease, and neurodegeneration. Neuronopathic Gaucher disease (nGD) encompasses two different forms of the disease, characterized by chronic or acute damage to the central nervous system (CNS). The cellular and molecular studies that uncover the pathological mechanisms of nGD mainly focus on lysosomal dysfunction since the lysosome is the key organelle affected in GD. However, new studies show alterations in other organelles that contribute to nGD pathology. For instance, abnormal accumulation of GluCer in lysosomes due to the loss of β-GC activity leads to excessive calcium release from the endoplasmic reticulum (ER), activating the ER-associated degradation pathway and the unfolded protein response. Recent evidence indicates mitophagy is altered in nGD, resulting in the accumulation of dysfunctional mitochondria, a critical factor in disease progression. Additionally, nGD patients present alterations in mitochondrial morphology, membrane potential, ATP production, and increased reactive oxygen species (ROS) levels. Little is known about potential dysfunction in other organelles of the secretory pathway, such as the Golgi apparatus and exosomes. This review focuses on collecting evidence regarding organelle dysfunction beyond lysosomes in nGD. We briefly describe cellular and animal models and signaling pathways relevant to uncovering the pathological mechanisms and new therapeutic targets in GD.

PCSK9 modulates cardiac metabolism and impacts HFpEF

Background and Aims: PCSK9 is a plasma glycoprotein mainly produced by the liver which, interacting with lipids and lipoprotein receptors such as LDLR receptor(s) and CD36, can regulate their degradation. PCSK9 deficiency induces an increase in the uptake of lipoproteins, promoting the removal of circulating lipids and their accumulation in different tissues. Given the impact of PCSK9 inhibitors as a modulator of plasma lipoprotein, levels the present work was aimed to evaluate its role on cardiac functionality and metabolism.

Verrüköz Karsinom ile Görülen Geç Teşhis Edilen Variegate Porfiri

Variegate porphyria (VP) is an autosomal dominant porphyria characterized by both cutaneous and neurovisceral symptoms. In this case we discuss a 65-year-old female patient with bullous lesions on hands, lethargy, increased facial hair and darkened facial skin. A form of acute porphyria, VP is caused by mutations in PPOX, a gene that carries instructions for making an enzyme called protoporphyrinogen oxidase. Here, we report a heterozygous mutation of the PPOX gene in a Turkish female VP patient who has been diagnosed with chronic renal failure secondary to porphyria. Porphyria is one of those disease needed to be kept in mind as differential diagnosis. Late diagnosis of this disease would lead to different chronic organ failures as a result of heme accumulation in different tissues and organs. This case, it is a clear reminder for the health care workers to remember the ''Primum non no nonocere'' rule and also remembering porphyria as a differential diagnosis in longterm attacks and symptoms.

Summary of four scientific studies on Arsenicum album high dilution effect against Arsenic intoxication in mice

Background: Groundwater arsenic affects millions of people in about 20 countries. In West Bengal (India) and Bangladesh alone over 100 million people are exposed. The arsenic concentration in contaminated groundwater in Bangladesh was above the maximum permissible level of 0.05 mg/l as recommended by WHO for developing countries [1]. Drinking water is not the only source of poisoning. In arsenic contaminated areas, crops, vegetables, cereals, poultry, cattle, etc, also contain traces of arsenic. Chronic arsenic intoxication has been associated with several diseases such as melanosis, leuco-melanosis, hyperkeratosis, oedema, skin cancer… &#x0D; Cazin et al [2], have demonstrated the effect of high dilutions of arsenic compounds. They noted increased arsenic elimination from blood through urine and faeces in intoxicated rats. According to these research, the aim of Khuda Buksh studies [3-4-5] was to investigate whether high dilution Arsenicum album have any effect on arsenic accumulation in different tissues and to understand also how this high dilution could produce a protective effect on all the different organs.&#x0D; Methodology: Firstly, the effect of Arsenicum album 30 cH on the amount of arsenic accumulation was determined by spectrophotometric analysis in four tissues namely liver, kidney and testis in mice intoxicated by arsenic. The protective effect in chronic and acute arsenic intoxicated mice of Arsenicum Album 6cH, 30cH and 200cH has been evaluated using not only the activities of enzymatic and biomarker toxicity (aspartate amino transferase (AST), alanine amino transferase (ALT), acid phosphatase (AcP), alkaline phosphatase (AlkP), lipid peroxidation (LPO) and reduced glutathione (GSH)) but also the cytogenetical parameters (chromosome aberrations (CA), mitotic index (MI), sperm head anomaly (SHA) etc., ). Because, it is well demonstrated that these enzymes biomarkers reflect the degree of hepatotoxicity and oxidative stress caused by arsenic intoxication.&#x0D; Results: Compared to controls, Arsenicum album 30cH induced a significant decrease in accumulation of arsenic in 4 tissues namely liver, spleen, kidney and testis in intoxicated mice. In addition, both Arsenicum album 6cH, 30cH and 200cH reduced chromosome aberrations, sperm head abnormality frequencies and activities of acid and alkaline phosphatases, aspartate and alanine aminotransferases and lipid peroxidation, while mitotic index and activities of glutathione, catalase and succinate dehydrogenase were increased compared to controls. &#x0D; Conclusion: Altogether, theses results provide evidence of protective potentials of the Arsenicum album dilution against acute and chronic arsenic intoxication in mice. They also offer a new hypothesis that the mechanism of the homeopathic dilution could act through regulation of expression of certain genes. This explanation seems to be plausible because all biomarker tests are regulated by specific genetic regulatory mechanisms [6].&#x0D; keywords: Arsenicum album, arsenic intoxication, enzymatic and biomarker toxicity.&#x0D;

Phenotypic Characterization and Determination of Gene expression of Genetically Modified Rice Strains Using CRISPR-CAS9 Technology With Sodium Chloride Effect

This study was conducted with the target of determine the role of OsHKT4 and OsHKT6 genes in rice plants under salt stress and observe its gene expression by GUS technology, as well as studying the Na+ and K+ accumulation in different tissues. The results obtained show that OsHKT4::GUS appeared strong GUS activity, expressed mainly in vascular tissues. In contrast, the GUS activity of the OsHKT6 promoters in NaCl-treated leaves was greater than that in water-treated leaves. Also in wild type plants, increasing the Na+ concentration has the effect of increasing the Na+ content of the tissues generally, the old leaves accumulating more Na+ which reduced the K+ content in roots and old leaves (Na+ levels are higher in the leaf lower parts). These results suggest that OsHKT4 and OsHKT6 genes plays a role in the accumulation of Na+ in old leaves, by adopting the mechanical exclusion of toxic ions in the old leaves of the plant.

Targeting Cd coping mechanisms for stress tolerance in Brassica napus under spiked-substrate system: from physiology to remediation perspective

Cadmium (Cd) is a prevalent, non-essential, carcinogenic, and hazardous heavy metal that reduces plant productivity and capacity of arable land area around the globe. In the present substrate-based pot study, seedlings of Brassica napus 180015 were grown equidistantly in the spiked-substrate medium for 60 days under increasing concentrations of Cd (0, 10, 20, 30, 40, 50 mg kg−1). Following harvest, the morpho-physio-biochemical, antioxidative, and Cd-induced tolerance responses were evaluated in B. napus under an increasing Cd stress regime. Additionally, these parameters were also investigated to select the plant's threshold tolerance limit for Cd under the spiked-substrate system. B. napus showed dynamic behavior regarding morpho-physio-biochemical attributes, including agronomic features, biomass, photosynthetic pigments, relative water content under increased Cd toxicity. Cd stress-induced hydrogen peroxide (H2O2) production with high MDA contents and passive EL, followed by the orchestration of both enzymatic (SOD, POD, APX, CAT, and GR) and non-enzymatic antioxidants (flavonoids, TPC, TPA, proline, and total soluble protein) up to a certain limit. In addition, Cd-induced stress upregulated transcriptional levels of antioxidative enzyme SOD, POD, APX, GR, and MT encoded genes in B. napus. The increasing trend of Cd accumulation in different tissues at the highest Cd concentration was as follows: root > leaf > stem. In spiked substrate system, B. napus demonstrated improved metal extractability performance and a high potential for phyto-management of low to moderate Cd contamination, implying that this study could be used for integrative breeding programs and decontaminating heavy metals in real contaminated scenarios. Novelty statement This study provides an insight into Cd-coping mechanisms of oilseed rape involved in alleviating toxicity and simultaneous phyto-management of increasing Cd concentration under spiked substrate system. The current study is the first scientific evidence of using a Cd-spiked soilless substrate medium. The present study will further strengthen our understanding of Cd-instigated positive responses in B. napus. Furthermore, it will provide a useful basis for integrative breeding programs and decontaminating heavy metals in real contaminated scenarios.

Tissue Distribution and Biochemical Changes in Response to Copper Accumulation in Erica australis L.

Copper uptake, accumulation in different tissues and organs and biochemical and physiological parameters were studied in Erica australis treated with different Cu concentrations (1, 50, 100 and 200 µM) under hydroponic culture. Copper treatments led to a significant reduction in growth rate, biomass production and water content in shoots, while photosynthetic pigments did not change. Copper treatments led to an increase in catalase and peroxidase activities. Copper accumulation followed the pattern roots > stems ≥ leaves, being roots the prevalent Cu sink. Analysis by scanning electron microscopy coupled with elemental X-ray analysis (SEM–EDX) showed a uniform Cu distribution in root tissues. On the contrary, in leaf tissues, Cu showed preferential storage in abaxial trichomes, suggesting a mechanism of compartmentation to restrict accumulation in mesophyll cells. The results show that the studied species act as a Cu-excluder, and Cu toxicity was avoided to a certain extent by root immobilization, leaf tissue compartmentation and induction of antioxidant enzymes to prevent cell damage.