Slaughterhouse Waste Research Articles

The Impact of Egbu Abattoir Wastes on Fungal Concentrates of the Soil Environment

The impact of Egbu abattoir wastes on fungal concentrates of the soil environment was undertaken. Samples of contaminated soil from the abattoir environment were taken against control and evaluated for fungal concentrates using standard methods. Results obtained showed that total fungal count (TFC) ranged from 3.5×105 cfu/g to 4.50×105 cfu/g and total hydrocarbon utilizing fungi (THUF) ranged from 1.8×105 cfu/g to 3.80×105 cfu/g. The percentage occurrence for mould isolates indicated the presence of Absidia sp., Aspergillus sp., Fusarium sp., Cladosporium sp., Penicillium sp., and Rhizopus sp. while yeast isolates were Candida sp., Rhodotorula sp., Saccharomyces sp., and Torulopsis sp., for both seasons. Though most of these organisms were indigenous to the soil some invading species were also isolated and were higher in contaminated soil than control soil. Egbu abattoir waste could be behind these observations. The study has revealed the impact of Egbu abattoir wastes on fungal concentrates of the soil environment.

The Effect of the Addition of a Fat Emulsifier on the Amount and Quality of the Obtained Biogas

Slaughterhouse waste management is an important technological, economic, and environmental challenge. Recently, more and more attention has been paid to the possibility of obtaining biogas from waste generated by slaughterhouses. The aim of the paper was to examine the effect of an emulsifier addition in the form of a carboxymethyl cellulose solution to create animal waste fermentation media based on the quantity and quality of the generated biogas. The adopted research goal was achieved based on a laboratory experiment of methane fermenting poultry processing waste. The waste was divided into two fractions: soft (tissue) and hard (bone). A fat emulsifier in a concentration of 1%, 2.5%, 5%, and 10% of fresh weight of the substrate was added to each substrate sample made from the above fractions. The emulsifier used was a 55% carboxymethyl cellulose solution, since this emulsifier is most commonly used in food production. The experiment was conducted in order to determine how the addition of an emulsifier (55% carboxymethylcellulose solution) affects the hydration of fats during methane fermentation, as demonstrated on poultry slaughterhouse waste. The samples were subjected to static methane fermentation, according to the methodology of DIM DIN 38414(DIN Deutches Institut für Normung). The experiment lasted 30 days. The total amount of biogas obtained after fermentation was 398 mL·g−1 for the soft fraction and 402 mL·g−1 for the hard fraction. In the case of the soft waste fraction, the addition of carboxymethylcellulose at 1% of the mass to the biogas process increased the amount of obtained biogas by 16%. In the case of the hard fraction, no effect of the addition of emulsifier on the total amount of biogas obtained was identified. In each case, the biogas from substrates with added emulsifier contained less methane and slightly more carbon. The emulsifier added to the soft fraction of slaughterhouse waste from poultry processing allowed cutting the process of methanogenesis by over 50% while maintaining the efficiency of biogas production. In the case of biogasification of bone tissue, no unambiguous effect of the addition of emulsifier on the improvement of process efficiency was identified.

Evaluation of slaughterhouse effluents and proposal of a recovery solution for Morocco

The present work consists in evaluating the waste potential of the slaughterhouse of Oujda (Morocco), to propose an innovative solution, which is the biogas technology or the biomethanisation. Slaughterhouse waste can beget significant health and environmental risks if not properly supported. Because they decompose rapidly and can carry pathogens and other contaminants.The anaerobic digestion process offers the possibility of combining the treatment of this waste with the production of a renewable energy that is biogas. The inoculum used for the digestion of this waste is maintained at the laboratory level in previous studies, it contains a diversified microbial consortium with a hydrolyzing strain to speed up the process. Various mixtures have been tested, and the best result has been obtained with: slaughterhouse waste + inoculum + algae.The parameters of anaerobic digestion have been monitored regularly (pH, composition and quantity of biogas, temperature ....).The biogas potential obtained under laboratory conditions, without continuous feed, is at least 5m 3 biogas/TSM. This first feasibility study at the laboratory level, allows us to conclude that this project is feasible and profitable. We propose the installation of a 15m 3 pilot bioreactor, which will be adapted and optimized before consider energy recovery totality of liquid and solid waste at the slaughterhouse level.This installation will have as main objective the production of biogas which will allow the heating of the sanitary water.

EFFECTIVE UTILISATION OF POULTRY BYPRODUCT MEAL AS AN INGREDIENT IN THE DIET OF GENETICALLY IMPROVED FARMED TILAPIA (GIFT), CULTURED IN RESERVOIR CAGES IN TAMIL NADU, SSOUTH INDIA

Evaluation of efficiency of poultry byproduct meal (PBM) as alternative protein source by replacing fish meal (FM) in the diets of GIFT strain of tilapia (Oreochromis niloticus) was carried out in cages installed in Poondi Reservoir, Tamil Nadu, South India. Six isonitrogenous (30% protein) and isolipidic (7% lipid) diets were formulated using graded levels of PBM protein to replace FM protein. Each diet was fed to two replicate groups of GIFT tilapia with mean initial weight of 20.38±0.0678 g, for 60 days in cages. The best growth performances in terms of mean weight gain (116.72 g), best food conversion ratio (FCR, 1.14) and maximum hepatosomatic index (HIS, 2.47) values were observed in GIFT tilapia fed PBM diet with 40% fish meal replacement. However, no significant (p>0.05) differences were observed in the whole body proximate composition of fish fed control and treatment diets. It was concluded that, poultry byproduct meal can replace 40% fish meal protein in the diets of GIFT tilapia cultured in reservoir cages without compromising growth, FCR and whole body proximate composition. The study suggests that poultry byproduct meal could effectively replace fish meal without affecting growth and feed conversion of the fish. The findings thus may pave a productive way for reducing environmental pressure of disposal of slaughter house waste.

Chemical composition of differently processed Cattle Hoof meal Waste as Feedstuff Ingredient

Waste generation at slaughter from ruminant has led to environmental concerns. Processing slaughter house waste will reduce the problem of disposal and possible utilisation in livestock feed. Subjecting Cattle hoof meal to different processing methods can help in enhancing its nutritive value. Cattle hoof were obtained from the slaughter house; raw hoof was subjected to processing methods by boiling; chemical treatment with 10 % soda ash + boiling; fermentation treatment in water + boiling; 10 % wood ash treatment in water + boiling; autoclave treatment and samples analysed for proximate composition, amino acid profile and mineral content analysis according to standard methods. The hoof proximal analysis ranged 9.30 ± 0.06 % – 12.39 ± 0.01 % moisture content; 0.34 ± 0.01 % – 2.50 ± 0.12 % ash; 0.31 ± 0.01 % – 1.47 ± 0.02 % crude fat; 0.19 ± 0.02 % – 12.71 ± 0.15 % crude fibre and 85.27 ± 0.20 % – 90.74 ± 0.26 % crude protein in all samples. The amino acids profile of the hoof showed significant difference among treated samples. Tryptophan an essential amino acid was below detectable limit in all processed samples and raw hoof. The essential and non-essential minerals content showed significant difference (P < 0.05) among treatments with highest (Mg, Fe, K) in wood ash; (Cu) in raw hoof; (Ca, Na, P, Mn, Zn) in autoclaved samples. This study, suggest that Cattle hoof has the potential of being exploited as a source of animal protein for feed formulation in animal nutrition. This research concludes that the different processing methods affect the nutritive profiles of treated samples hence supplementation of limiting amino acids envisaged.

Mapping of biogas production potential from livestock manures and slaughterhouse waste: A case study for African countries

Despite the remarkable potential for biogas production from livestock and organic wastes, the number of constructed biogas digesters for the continent is in the order of thousands and in case of Mauritania is lower than the number of the provinces. While majority of the existing digesters left far behind the expected efficiency, lack of a comprehensive economic assessment along with an efficient design questions their practical application. The present study introduced the first assessment to evaluate the biogas potential from livestock manures and waste from slaughterhouses in Northwest Africa (a case of Mauritania). Using ArcGIS® software, a database was conducted to build maps that show the amount of waste products, the potential of biogas, and equivalent amounts of energy. These were used to assess the potential of biogas and the corresponding potential energy for each geographical department in Mauritania. The results indicated that the southern provinces had the highest biogas potential with the maximum and the average values of 520 and 258.7 (±125.8) × 106 m3/year. On the other hand, the lowest biogas production potential (27.7 × 106 m3/year) was recorded for northern provinces with the maximum and the average values of 135 and 76.4 (±39.7) × 106 m3/year. The results showed that 63,579 × 106 kg of waste associated with livestock (cattle) and slaughterhouse applications were annually produced in the country. It was determined that this quantity could generate 2451 × 106 m3 of biogas per year, corresponding to an energy potential of 52,704 × 106 MJ/year. Considering the rapid depletion of conventional energy sources and the significance of biogas as a renewable fuel, a detailed feasibility analysis (for the biogas production in each province of Mauritania by means of community type fixed-dome digesters) was also performed in the scope of this study. The results of the comprehensive cost breakdown analysis revealed that the revenues obtained from sale of biogas-generated electricity and digested slurry (as fertilizer) could able to pay the initial investment within approximately 6.5 years without subsidy. The findings of this study, as the first of its own, could be used to comprehend how utilization of information such as slaughterhouse and livestock population, nutrition habitats, land-usage maps and geographic information system (GIS) can be employed to germinate a model for more comprehensive assessment of biogas production potential from livestock manure and slaughterhouse wastes, specifically in case of northern African countries. Moreover, in case of biogas plant, this model could be employed as a decision-making tool to identify the highly qualified location for construction of the biogas plant.

Potential of Abattoir Waste for Bioenergy as Sustainable Management, Eastern Ethiopia, 2019

Our environment is facing serious problems of high volumes of waste generation and inadequate disposal system in worldwide particularly in developing countries. There is also lack of studies on quantification of abattoir waste and lack of workers awareness towards abattoir waste. Therefore, the purpose of the study was to estimate abattoir waste for bioenergy potential as sustainable management. A cross-sectional study was conducted in four selected abattoirs of Eastern Ethiopia from January 1st, 2018 to December 30th, 2018. The magnitude of abattoir waste composition was computed based on Aniebo mathematical computational from the actual number of slaughtered livestock. The study demonstrated that four selected abattoirs generate 1,606.403 ton of abattoir waste per year and using anaerobic digestion of about 85,139 m3/year of biogas and 111.25 ton/year of biofertilizer can be produced. The biogas or energy from the waste can replace firewood and charcoal and the expensive fossil fuels. Using Banks mathematical computation about 20,054.12 m3/year production of biogas could replace 20.56 ton/year of energy consumed by liquefied petroleum gas, kerosene, charcoal, furnace oil, petrol, and diesel in average. The current estimated biofertilizer (111.25 ton/year) from four abattoir sites can cover about 2,225 hectares/year with its advantage and efficiency of soil. When turned into cost, about $55,645 per year of price could estimate from biogas and biofertilizer. The study concluded that huge amount of biogas and dry biofertilizer yields could produce from abattoir waste through anaerobic digestion. Therefore, installing anaerobic digestion plant is recommended to ensure environmental safety and public health.

Production of Biogas and Bio-fertilizer from Abattoir Waste as Sustainable Management, Eastern Ethiopia, 2019

Our environment facing by serious problems of high volumes of waste, inadequate disposal system particularly in developing countries worldwide. There is also lack of studies on quantification of abattoir waste and lack of workers awareness towards abattoir waste. Therefore, the purpose of the study was to estimate abattoir waste for bioenergy potential as sustainable management. A cross-sectional study was conducted in four selected abattoirs of Eastern Ethiopia from January 1st, 2018 to December 30th, 2018. The magnitude of abattoir waste composition were computed based on Aneibo mathematical computational from the actual number of slaughtered livestock. The study demonstrated that four selected abattoirs generates 1,606,403kg of abattoir waste per year and using Fed batch Aerobic digester about and 85,139m3/year of biogas and 111,249kg/year of bio-fertilizer can be produced. The biogas or energy from the waste can replace firewood and charcoal and the expensive fossil fuels. Using Banks mathematical computation about 85,139.20m3/year of biogas estimated, could cover 36097.43kg of liquefied gas or 48129.89kg of kerosene or 280757.68kg of charcoal or 32086.59kg of furnace oil or 56151.5kg of petrol or 40108.2kg of diesel per a year in same functions. The current estimated bio-fertilizer (111,249kg/year) from four abattoir sites can cover about 2,225 hectares per a year with its advantage and efficiency of soil. When in turned into cost, about $55,645 per a year of price could estimate from biogas and bio-fertilizer. The study concluded that huge amount of biogas and dry bio-fertilizer yields could produce from abattoir waste through fed batch anaerobic digestion. Therefore, install fed batch anaerobic digestion plant is recommended to ensure environmental safety and public health. Keyword : Abattoir waste, Biogas, Bio-fertilizer, Sustainable management

Effect of different organic substrates on reproductive biology, growth rate and offtake of the African night crawler earthworm (Eudrilus eugeniae)

Rapid growth and high fecundity of E. eugeniae makes it a commercial vermicomposting agent. The worm is also a rich protein source (50-70%CP) in livestock diets. The major question, however, is how do we promote earthworm production as a strategy for ecological livestock intensification and integration with crops through earthworm domestication as a source of protein and vermicompost. Reproduction characteristics, growth and offtake of E. eugeniae were studied using four organic substrates including abattoir waste (AW), cattle manure (CM), soya bean crop residue (SBCR) and a mixture of cattle manure and soya bean crop residue (CM+SBCR) aged for 15 days. Irrespective of the substrate, length and biomass of earthworms increased at a decreasing rate between the 1st and 8th weeks. Clitellum appearance was initiated at 31.5±2.4, 32.8±3.2, 33.7±3.3 and 35.5±2.4 days for AW, CM, CM+SBCR and SBCR, respectively, while cocoon initiation was at 69.0±1.4 (AW), 54.9±2.3 (CM), 51.7±1.7 (CM+SBCR) and 60.0±2.4 (SBCR) days. Organic substrate used affected reproductive biology, growth and offtake of E. eugeniae. Higher survivability, total earthworm biomass accumulation and offtake when cultured on CM and a binary combination of CM + SBCR is an indication that a strategy for sustainable crops-livestock integration can be sparked off by earthworm domestication. Earthworm domestication can be promoted using CM or a combination of (CM+SBCR) as substrate.

Research Progress of Energy Utilization of Agricultural Waste in China: Bibliometric Analysis by Citespace

Energy utilization of agricultural waste, due to the depletion of petroleum resources and the continuous deterioration of the ecological environment, has become an increasingly important development area at present, with broad prospects. The Citespace software was used to systematically summarize the research hotspots, development, and frontiers of researches on the energy utilization of agricultural waste in China from 1999 to 2018. The results show that (1) the number of publications in this field has increased, which includes a steady development stage, a rapid development stage, and a fluctuation and decline stage. (2) Research hotspots focused on technology for energy utilization of agricultural waste, benefits analysis of energy utilization of agricultural waste, energy conversion and upgrading path of agricultural waste, and energy potential of agricultural waste. (3) Development of research hotspots go through five stages: “technology for energy utilization of straw and the disposal of livestock and poultry waste”, “exploration of energy utilization mode of agricultural waste and the disposal of by-product from energy utilization of agricultural waste”, “technology upgrading from agricultural waste to fuel ethanol and recycling of livestock and poultry waste”, “resource recycling of by-product from biogas ” and “energy utilization of livestock and slaughterhouse waste”. It has revealed the focus in this field was changing from planting waste to breeding waste, and from unprocessed waste to by-product from energy utilization. (4) Energy utilization of slaughterhouse waste and cow manure has started to be considered as the frontiers of researches.

Enzyme assays and toxicity of pig abattoir waste in Eisenia andrei

Due to high global demand, large amounts of abbattoir waste are generated from pork production. Mismanagement of abattoir waste on agricultural lands can result in soil and water contamination with pathogens and contaminants like metals and nutrients. Therefore, possible effects on soil organisms prior to application should be evaluated. Thus, the aim of this study was to determine the effects of fresh pig abattoir waste (PAWf) and waste after stabilization processes on E. andrei through tests of avoidance behavior, acute toxicity and chronic toxicity. In order to do this, the waste was evaluated fresh (i.e., non-treated), and after aerated composting (PAWa), natural composting (PAWn) and vermicomposting (PAWv). In addition, we used a natural soil with no history of agricultural use as control soil. The evaluation was based on avoidance behavior, mortality, initial and final earthworm weight, and reproduction, in addition to a set of enzyme assays formed by acetylcholinesterase, lipid peroxidation, catalase and glutathione S-transferase measured over time. The ecotoxicological results showed that PAWf and PAWa increased AChE activity at different experimental periods, while PAWn decreased activity at 14 days compared to the control. PAWf and PAWa increased TBARS levels at 7 and 14 days, respectively. CAT activity decreased at 3, 7 and 14 days in PAWv, while GST activity increased at 3 days in PAWa and at 3 and 14 days in PAWf compared to the control. In the acute toxicity test, PAWa and PAWn had a toxic effect on E. andrei, resulting in 100% mortality at 14 days of exposure. Based on our findings, pig abattoir waste should undergo vermicomposting prior to agricultural application to soils.

Spatial Distribution of Bacterial Isolates from Different Abattoirs in Port Harcourt, Nigeria

The constituents of wastes generated from abattoir activities create conducive environment for microbial proliferation, most of which are pathogenic. Infections caused by these microorganisms could result to zoonoses. This study was to determine the distribution of bacterial isolates and their biomass from different abattoirs in Port Harcourt. Samples like waste blood, table swab, service water, faecal matter, soil and wastewater from abattoirs in Iwofe, Rumuodomaya and Trans-Amadi were collected from October 2017 to November, 2018 and analysed using standard microbiological procedures. Results obtained revealed that the total heterotrophic bacterial count of blood samples ranged from 8.33x101 to 3.33x102 cfu/ml for Trans-Amadi and Iwofe abattoirs, table swabs ranged from 6.74x104 to 4.88x106 cfu/ml, water samples ranged from 1.56x104 to 2.07x104 cfu/ml; faecal matter had THB counts ranging from 9.97x107 to 1.06x108 cfu/g; and soil samples ranged from 1.11x1010 to 1.17x1010 g, while wastewater counts ranged from 1.03x108 to 1.08x108 cfu/ml. The predominant Bacterial isolates were of the genera Micrococcus, Staphylococcus, Serratia, Pseudomonas, Proteus, Klebsiella, Escherichia and Chromobacterium, Serratia sp. only was isolated from Iwofe and Rumuodomaya abattoirs within April to October while Chromobacterium sp. was isolated in Trans-Amadi and Rumuodomaya abattoirs within the months of May to October. Among the isolates, Escherichia coli and Klebsiella species occurred more compared to others in all the three locations. A higher percentage of microorganisms were recorded in the month of May compared to other months. It is presumed that abattoir wastes harbour many microorganisms of public health importance. The occurrence of these microbes, most of which are enteric pathogens, poses a public health challenge as infections by them could result in illnesses such as gastroenteritis, septicaemia and pneumonia especially in the absence of good hygiene around abattoirs. Proper sanitation in abattoirs as well as management of abattoir wastes is important in reducing the spread of these microorganisms.

Virulence Genes, Antibiotic Susceptibility Profiles and Molecular Characterization of Campylobacter Species Isolated from Different Slaughterhouses

The aim of this study was to investigate the frequency of Campylobacter species, to detect the antibiotic resistance profiles and the virulence genes and to determine the clonal proximity of the isolates in the samples of cutting board, slaughterhouse waste water, wall, knife and carcass from three different slaughterhouses in Kayseri region. For this purpose, a total of 150 samples, 10 of each from knife, wall, cutting board, carcass smear sample and slaughterhouse wastewater were collected from each of the three types of slaughterhouses in 2018 in Kayseri. For the isolation of the Campylobacter species, following preenrichment, the suspensions were inoculated onto modified charcoal cefoperazone desoxycholate (CCD) agar and were incubated at 37°C under microaerophilic condition for 48-72 hours. Suspicious colonies with gray-white color were recovered and subjected to phenotypical (Gram staining, oxidase, catalase test, and motion test) tests. Multiplex polymerase chain reaction (mPCR) was used for the molecular identification of the Campylobacter species. Antimicrobial susceptibilities of the isolates identified at the species level were detected by using the disk diffusion test and antibiotic gradient test. Virulence genes (iam, cadF, cdtA, flaA, ceuE, cdtC, cdtB and virB11) among the isolates were evaluated by PCR. The molecular typing of the isolates determined at species level was performed by Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR). In the study, 17 (11.3%) of the 150 samples taken from the slaughterhouse were found to be suspicious in terms of Campylobacter spp. and as a result of phenotypic identification tests, all of the isolates were verified as Campylobacter spp.. As a result of mPCR; eight of the isolates were identified as Campylobacter jejuni, eight as Campylobacter fetus and one as Campylobacter coli. The isolation of the Campylobacter species from different sources was found to be higher in slaughterhouse wastewater than those of others (p<0.001) and the difference in the proportional distribution of the Campylobacter species obtained from various sources was statistically significant (p<0.05). As a result of the disk diffusion test, while, all C.jejuni isolates were resistant to ciprofloxacin, 87.5%, 25%, 25% and 12.5% of C.jejuni isolates were resistant to enrofloxacin, neomycin, amoxicillin/clavulanic acid, and erythromycin, respectively. In addition, 25%, 25% and 12.5% of C.fetus isolates were resistant to amoxicillin/clavulanic acid, neomycin and gentamicin, respectively. C.coli isolate was not resistant to any of the antibiotics tested. Antibiotic gradient test results were found to be compatible with the disc diffusion test results. One of the virulence genes examined, virB11, was not detected in any of the isolates. Moreover, iam gene was not present in C.fetus and C.coli isolates, but only in one C.jejuni isolate. The flaA gene was detected in six C.jejuni isolates. C.coli isolate and seven C.jejuni and seven C.fetus isolates were positive in terms of the cdtC gene. The cdtA, cdtB, ceuE and cadF genes were found to be positive in all C.jejuni isolates. All isolates analyzed in the study demonstrated different ERIC-PCR profiles. In conclusion, it was shown that Campylobacter strains isolated from slaughterhouses were resistant to the most of the current antibiotics. Moreover, the presence of highly virulent Campylobacters in the slaughterhouse environment threatens public health due to the risk of contamination of the humans via carcasses and foods. Therefore, it is recommended that strict hygiene rules should be followed to reduce Campylobacter species contamination in slaughterhouses.

Production, disposal, and efficient technique used in the separation of heavy metals from red meat abattoir wastewater.

The availability of clean water from the already scarce sources is threatened by continuous addition of contaminated industrial and of abattoir waste into watercourses globally. The aim of the current study was to reduce the amount of waste produced, to decrease pollution derived from discharge of effluent meat wastewater, and also to minimise environmental health risk. This was all achieved by successfully synthesising a natural biopolymer chitin chitin-chitosan derivative derived from crab shell waste. Characterisation of the chitin polymer using physicochemical properties like yield, ash content, degree of acetylation, solubility, intrinsic viscosity, and molecular weight indicated that crab shell waste was a good source of chitin. This polymer was later deacetylated to form chitosan and then cross-linked with s-methylbutylamine to form chitin-chitosan adsorbent. The effectiveness of the above-modified product in purifying meat wastewater was conducted by means of comparative testing using hydride gas atomic absorption spectroscopy. The results of the qualitative and quantitative analysis showed that chitosan cross-linked s-methylbutylamine was efficient in removing the following metal ions: Cu (II), Zn (II), Cr (II), Pb (II), and Cr (II). The highest removal percentage was Cr (IV) and Pb (II) (95.45 and 92.66%), while Zn (II) and Fe (II) were 87.32 and 67.48%. The lowest percentage recovery of 38.55% was observed for the metal Cr. From the current study, it was evident that the cross-linked can significantly reduce the metal concentration in meat wastewater before it is released in the aquatic environment. Therefore, properly permitted international waste disposal methods should be employed to reduce adverse effects on the receiving environment.

Evaluating the level of ammonia and sulfide in the liquid phase during anaerobic digestion of slaughterhouse waste operating at mesophilic scale digester—the impact of inhibition and process performance

The performance of experimental batch-reactor loaded with slaughterhouse waste at mesophilic temperature was investigated as well as the inhibition of both ammonia and sulfide concentration in the aqueous phase. The digester was operated for 68 days by evaluating the process stability basing on controlling parameters such as pH, volatile fatty acids and alkalinity in relation to the methane produced. The maximum CH4 content of 69.6% was achieved at 0.37 VFA/Alkalinity ratio and pH of 7.51 during day 37 of anaerobic digestion. However, a sudden increase of ammonia nitrogen in the digester from day 44 to day 68 decreased the methane content about 62.15% from 65% to 24.6%. Similarly, as the amount of sulfide content decreased in the liquid phase, gaseous H2S was elevated up to 132 ppm in the 68th day. During this time, it was observed that the ratio of VFA/Alkalinity decreased to 0.16, with a very low concentration of VFA, which was 150.92 mg/L. This phenomenon indicated that all the acids produced were consumed by methanogens and ammonia inhibition was at the highest rate due to the increase of ammonia nitrogen concentration in the last days of digestion. Furthermore, among of peculiar characteristic shown by slaughterhouse waste is the ability to maintain the pH above 7 without the addition of any buffering agent throughout the AD process. Meanwhile, the evaluation of the level of both ammonia and sulfide in the aqueous phase revealed that the inhibitory effect of ammonia concentration was higher than sulfide concentration.

Impact of Abattoir Wastes on Trace Metal Accumulation, Speciation, and Human Health–Related Problems in Soils Within Southern Nigeria

The slaughtering of animals and processing of meats for human consumption generates enormous wastes which are not properly managed in most developing nations including Nigeria. Majority of people in Akwa Ibom state in southern Nigeria depend on meat as their major source of protein, and abattoir wastes are applied in farms as organic manure by some farmers. This study examined the role of abattoir-related waste products in the physicochemical properties, total metal, and metal speciation of the soil. The data obtained were also subjected to some treatments using some environmental models to establish the degree of contamination by the parameters determined, studied locations, and the associated human health problems. Samples were collected from 5 designated abattoirs in Akwa Ibom state. Thirty composite samples were used for the research. Results obtained showed higher levels of pH, electrical conductivity, organic matter, and cation exchange capacity in the abattoir waste–impacted soils than in the control plot. Levels of pseudo total heavy metals were also higher in the studied soils than in the control plot. The mean values of the metals are below 400, 85, 140, 36, 100, and 35 mg/kg of recommended limits for Fe, Pb, Zn, Cu, Cr, and Ni, respectively by the Federal Environmental Protection Agency (FEPA) in Nigerian soil. The results also revealed that Fe and Cr existed mainly in residual fraction. However, Zn, Cu, and Ni existed principally in the form bound to organic matter/sulfide. In addition, we detected that Pb existed mainly in the reducible fraction. Disparities were also observed in the speciation results of the metals between the studied soils and the control plot. Principal component analysis (PCA) indentified that both the geogenic and anthropogenic factors contributed to the accumulation of metals determined in the studied soils. Variable relationships were also observed for the heavy metals determined in the studied soils. Fe showed a high-risk potential, and children were more vulnerable due to its toxicity. We conclude this study was able to expose the consequences of indiscriminate dumping of abattoir wastes on the quality of soil and the associated human health problems.

Survey on UHF Device Operating Modes for Thermal Treatment and Disinfection of Nonfood Animal-Origin Raw Materials

The authors have developed ultra-high-frequency (UHF) devices of several types that are different in their operation and design principles. It is well known that the technological process of the animal product treatment includes a range of operations resulting in obtaining the final products and nonedible by-products approved for livestock feed manufacturing. Therefore, the development of technologies and technical means for treatment of slaughterhouse waste classed as nonedible animal by-products, which can provide the opportunity to make the processed protein product, is considered urgent. For this reason, surveys on UHF devices’ operating modes for thermal treatment of slaughterhouse waste were carried out. A description of the continuous mode operating principle of a UHF device combined with a resonator for the thermal treatment of nonfood by-products of raw meat material is reported. The combined resonator designed to ensure the electromagnetic safety limits is presented as a variation of a cylindrical resonator with a hemispherical base providing the raw material supply. The regression-based models optimizing the UHF device operating modes are developed. A description of the technology and the device for thermal treatment of the nonfood animal-origin raw materials under the impact of high-frequency electromagnetic fields, operating in the continuous mode, is reported. The properties of dielectric heating of the multicomponent raw material during its grinding in the resonator chamber and the analogs of the HVF devices are considered.

Effects of Abattoir Waste on the Surface Water Quality of Dei-Dei River, Abuja, Nigeria

This study has investigated the Effects of Abattoir Waste on the Surface Water Quality of Dei-Dei River, Abuja. It assessed the impacts of abattoir waste disposal on Dei-Dei River, biological properties such as E-coli, Faecal Streptococci, Total Coliform Count (TCC) and Cryptosporidium Oocyst (Co), with results compared with WHO standards. Field methods included consultation with Health and Water Quality Expert, collection of water samples at varying distance and laboratory analysis of water samples using standard techniques. The analysis at the upstream, revealed that at point 1, E.coli was 2.00cfu/ml above 0 cfu/ml and TCC was 18cfu/ml above 10 cfu/ml benchmark for raw water respectively. At Point 2, E.Coli was 1.00 cfu/ml above 0 cfu/ml while TCC is 12.00 cfu/ml. At Point 3, E. Coli was 1.00 cfu/ml above 0 cfu/ml while TCC is 20.00 cfu/ml and above the bench mark for raw water. The midstream of the study area, revealed that at Point 1, E.coli was 3.00cfu/ml above 0 cfu/ml and TCC was 25cfu/ml above 10 cfu/ml benchmark for raw water respectively. At Point 2, E.Coli was 2.00- cfu/ml above 0 cfu/ml while TCC is 22.00 cfu/m, Co 1 log reduction l. At Point 3, E. Coli was 1.00 cfu/ml above 0 cfu/ml while TCC is 9.00 cfu/ml. The result of the analysis of sample collected at the downstream of the study area revealed that at Point 1, E.coli was 5.00cfu/ml above 0 cfu/ml and TCC was 32cfu/ml above 10 cfu/ml, Co 2 log reduction and within the benchmark for raw water respectively. At Point 2, E.Coli was 3.00 cfu/ml above 0 cfu/ml while TCC is 38.00 cfu/ml, Co1 log reduction and within the Benchmark for raw water respectively. At Point 3, E. Coli was 1.00 cfu/ml above 0 cfu/ml while TCC is 35.00 cfu/ml and Co 0.00 log reduction and within the benchmark for raw water respectively, while at Point 1 and Point 2, E.Coli and TCC were above the bench mark for raw water. Presence of these micro-biological components are indications that the surface water of the study area may likely be responsible for diseases such as typhoid fever, diarrhoea and cholera if used for drinking purpose. The study has also made recommendations to address the problems revealed such as; improvement of sanitation of the study area, provision of adequate waste management facilities while sensitization of the people is given priority, ensuring clean water in the area and efforts to stop dumping of Abattoir waste into the river and at the river bank.

PENGOLAHAN LIMBAH CAIR RUMAH POTONG HEWAN MENGGUNAKAN ROTATING BIOLOGICAL CONTACTOR MODIFIKASI SLUDGE ZONE

Slaughterhouse wastes generally contain blood, proteins, fats, and suspended solids that cause a high load of organic matter which can pollute rivers and water body. The waste water of slaughter house will cause changes on the quality of the water, such as increased colour, pH, total dissolved solids, suspended solids, fats, BOD5, ammonium, nitrogen and phosphor. In this study aims to reduce the content of slaughterhouse waste water, using Rotating Biological Contactor (RBC) modified with a sludge zone. It was expected that by modifying the sludge zone, the efficiency of RBC performance can be further improved. This study used a variety of Hydraulic Retention Time (HRT) of 2.8 hours, 3.5 hours and 4.2 hours along with waste water dilution of 50%, 75% and 100% using the continue system into clarifier process. Based on the results of the study, the decreased percentage in COD concentration reached 86.68%, the total decreased percentage of Nitrogen concentration reached 66.11% and the decreased percentage in TSS concentration reached 71.87%. The pH value is neutral with a range of 7.6-8.3 with temperatures range of 26oC-30oC during operation. The highest DO values reached 5.2 mg/L with a range of organic surface loading values of 89.77 - 135.45 grams BOD/m2 .day. The organic load figure exceeds the RBC organic load standard which averages around 8-20 grams/BOD/m2.day. The increased of organic load, indicates that RBC with modified sludge zone was able to treat wastewater with high organic load, such as slaughterhouse waste water.

Anaerobic Digestion Creating Renewable Energy-The Ultimate Closed Loop System

This paper clarifies the significance of Anaerobic Digestion (AD) process. Sustainable power source from anaerobic assimilation gets little evaluation in the press when contrasted with other standard inexhaustible power age advances. It has not been so well known a sustainable power source when contrasted with hydropower sustainable power source or wind sustainable power source in the course of the most recent couple of years. Be that as it may, Renewable vitality from anaerobic processing is boosted the innovation will turn out to be increasingly mainstream in the coming years. Sustainable power source from anaerobic assimilation is amassed in America and Europe and eminently famous in India. Each 1 ton of sustenance waste discarded unnecessarily is in charge of 4.5 huge amounts of CO2 proportionate emanations. Advertisement gives a neighbourhood reasonable secure vitality source free of worldwide financial vitality changes and accessibility, where income is kept in the nearby economy as opposed to going to oil rich nations and multinationals. Sustainable power source from anaerobic processing is created by the consuming of methane. Sustainable power source from anaerobic assimilation is created in storehouses where specific microbes are added to natural waste. Sewage, vegetation, excrement, slaughterhouse waste and waste water would all be able to be separated in an anaerobic assimilation storehouse. At times, specific silage yields are developed for decay. The microbes are added to the waste and the disintegration happens without oxygen. The methane delivered during decay is scorched nearby, driving turbines and making inexhaustible power. Anaerobic processing isn't especially reasonable for little scale local sustainable power creation, to a great extent because of the space prerequisites for the storehouses and the sheer measure of waste required to delivering methane. Notwithstanding, sustainable power source from anaerobic assimilation can be delivered on a huge business scale, a training regular in the United States, taking waste from a wide area.