Bioglass Research Articles

Two-step sinter-crystallization of K2O–CaO–P2O5–SiO2 (45S5-K) bioactive glass

Bioactive glasses and glass-ceramics (GCs) effectively regenerate bone tissue, however most GCs show improved mechanical properties. In this work, we developed and tested a rarely studied bioactive glass composition (24.4K2O-26.9CaO-46.1SiO2-2.6P2O5 mol%, identified as 45S5-K) with different particle sizes and heating rates to obtain a sintered GC that combines good fracture strength, low elastic modulus, and bioactivity. We analyzed the influence of the sintering processing conditions in the elastic modulus, Vickers microhardness, density, and crystal phase formation in the GC. The best GC shows improved properties compared with its parent glass. This glass achieves a good densification degree with a two-step viscous flow sintering approach and the resulting GC shows as high bioactivity as that of the standard 45S5 Bioglass®. Furthermore, the GC elastic modulus (56 GPa) is relatively low, minimizing stress shielding. Therefore, we unveiled the glass sintering behavior with concurrent crystallization of this complex bioactive glass composition and developed a potential GC for bone regeneration.

Hypoxia-mimicking 3D bioglass-nanoclay scaffolds promote endogenous bone regeneration

Large bone defect repair requires biomaterials that promote angiogenesis and osteogenesis. In present work, a nanoclay (Laponite, XLS)-functionalized 3D bioglass (BG) scaffold with hypoxia mimicking property was prepared by foam replication coupled with UV photopolymerization methods. Our data revealed that the incorporation of XLS can significantly promote the mechanical property of the scaffold and the osteogenic differentiation of human adipose mesenchymal stem cells (ADSCs) compared to the properties of the neat BG scaffold. Desferoxamine, a hypoxia mimicking agent, encourages bone regeneration via activating hypoxia-inducible factor-1 alpha (HIF-1α)-mediated angiogenesis. GelMA-DFO immobilization onto BG-XLS scaffold achieved sustained DFO release and inhibited DFO degradation. Furthermore, in vitro data demonstrated increased HIF-1α and vascular endothelial growth factor (VEGF) expressions on human adipose mesenchymal stem cells (ADSCs). Moreover, BG-XLS/GelMA-DFO scaffolds also significantly promoted the osteogenic differentiation of ADSCs. Most importantly, our in vivo data indicated BG-XLS/GelMA-DFO scaffolds strongly increased bone healing in a critical-sized mouse cranial bone defect model. Therefore, we developed a novel BG-XLS/GelMA-DFO scaffold which can not only induce the expression of VEGF, but also promote osteogenic differentiation of ADSCs to promote endogenous bone regeneration.

Understanding Crystallization, Mechanical Properties and Reactivity of Multicomponent Bioactive Glasses Through Molecular Dynamics Simulations

In this study, we have applied Molecular Dynamics (MD) simulations to obtain reliable structural models of the bulk and surfaces of the 45S5 Bioglass® and three recently designed glasses in the system Na2O-K2O-CaO-MgO-SrO-P2O5-SiO2 and interpreting their different behaviour and properties. In fact, the substitution of sodium oxide with alkaline earth oxides (Ca, Mg and Sr) in the traditional 45S5 Bioglass® has been shown to be beneficial for improving the sinterability and mechanical properties of the glass maintaining optimal biocompatibility.The reduced tendency of crystallization has been associated to the increased chemical disorder around network former cations, the reduced mobility of alkaline earth cations with respect to sodium and the reduced coordination number of magnesium, which hamper the large structural re-organization necessary to the precipitation of the Na2Ca2Si3O9 crystal phase. In all the compositions, fragmented chains dominate the silica network and the network connectivity is similar. However, the increased interconnection of such chains by Mg, Ca and Sr cations makes the substituted glasses stiffer and harder than the 45S5 Bioglass®. The different reactivity of the glasses is due to the different amount of alkali cations and undercoordinated species at the glass surfaces.The approach presented provides deeper insights on the structure-properties relationships in bioactive glasses, and can be used to find precious guidelines for compositional tuning and designing innovative bioactive glass compositions with desired behaviour or property for a specific application.

Deepening our understanding of bioactive glass crystallization using TEM and 3D nano-CT

One key issue influencing a broader application of Bioglass 45S5 in tissue engineering is its inherent crystallization tendency, severely limiting the mechanical strength of 3D porous scaffolds. Despite numerous studies, Bioglass 45S5 crystallization is not yet fully understood with regard to the mechanisms involved or morphology of the crystal phases forming. Here we show how two cutting-edge imaging techniques, state-of-the-art transmission electron microscopy (TEM) with image correction including energy dispersive X-ray spectroscopy and X-ray nano-computed tomography (nano-CT), allowed us to visualize changes in microstructure from near-nucleation to almost full crystallization in bulk Bioglass 45S5. At early times of heat treatment at 660 °C the formation of phase-separated nano-droplets within the glassy matrix was observed. Later, besides surface crystallization, bulk crystallization of combeite spheres was predominant. The formation of the first combeite spheres, their coarsening with time and finally their merging at near full crystallization were recorded by in situ high-temperature optical microscopy videos. The 3D nature of these spheres was confirmed by nano-CT, while TEM showed that their internal structure was composed of sub-micron grains. X-ray diffraction analysis at early time points showed a much higher crystalline fraction in bulk samples compared to powder samples, highlighting the influence of processing and sample morphology. These results show the importance of using complementary techniques for gaining insight into the crystallization process in the volume. In addition, we show that TEM and nano-CT are suitable characterization techniques to visualize the crystallization even in fast crystallizing systems, such as bioactive glasses.

Structural, thermal, morphological and dielectric investigations on 45S5 glass and glass-ceramics

Hench et al. developed in 1969 the 45S5 Bioglass® showing high biocompatibility and osteogenic ability, enabling its application in bone tissue engineering. However, the bioactivity level and other physical and chemical properties can be compromised by structural changes. It is also known that the bone growth kinetics is related to the electrical characteristics of the bioglass. This manuscript presents an extensive study on the structural characteristics of the 45S5 Bioglass® endorsing the electrical properties with the structural and morphologic changes established by controlled heat-treatments. The bioglass was synthesized by melt-quenching and the heat-treatments were made at several temperatures from 500 up to 1000 °C. XRD, Raman and FTIR spectra show the occurrence of glass crystallization for treatments above 600 °C, in agreement with the thermal analysis of the base glass. The main phase, in the glass-ceramic samples, is Na2Ca2Si3O9, presenting a secondary phase in the sample treated above 900 °C. SEM micrographs show nanosize particles in the glass-ceramics, except for the sample treated at 1000 °C, which presents submicron-size. The electrical measurements revealed that the crystal phase dominates the conduction process and the dipoles arising from the interfaces lead to relaxations phenomena.

Effect of bioactive glass air-abrasion on Fusobacterium nucleatum and Porphyromonas gingivalis biofilm formed on moderately rough titanium surface.

This aim of this study was to investigate the effects of three types of air-abrasion particles on dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis, both of which were cultured on sandblasted and acid-etched (SA) titanium discs. Out of 24 SA discs with biofilm, 18 were exposed to either air-abrasion using Bioglass 45S5 (45S5 BG; n=6), novel zinc (Zn)-containing bioactive glass (Zn4 BG; n=6), or inert glass (n=6). The efficiency of biofilm removal was evaluated using scanning electron microscopy (SEM) imaging and culturing techniques. Air-abrasion using 45S5 BG or Zn4 BG demonstrated a significant decrease in the total number of viable bacteria compared to discs air-abraded with inert glass or intact biofilm without abrasion. Moreover, P.gingivalis could not be detected from SEM images nor culture plates after air-abrasion with 45S5 BG or Zn4 BG. The present study showed that air-abrasion with 45S5 or Zn4 bioactive glasses can successfully eradicate dual-biofilm of F.nucleatum and P.gingivalis from sandblasted and acid-etched titanium discs.

Biosilicate® Glass-Ceramic Foams From Refined Alkali Activation and Gel Casting

Biosilicate® glass-ceramics are among the most valid alternatives to 45S5 Bioglass. They combine a similar bioactivity and bioresorbability as the 45S5 with superior mechanical strength, owing to the crystallization of a Na–Ca silicate phase. This crystallization may be experienced upon viscous flow sintering of fine glass powders, thus configuring a sinter-crystallization process. As crystallization is seldom complete, sintering can also be applied to semicrystalline powders. The sintering/crystallization combination may be exploited for shaping highly porous bodies, to be used as scaffolds for bone tissue engineering, in the form of foams. The present study aims at exploring a gel-casting process, based on the room temperature foaming of powders suspended in a “weakly alkaline” (1 M NaOH) aqueous solution, followed by sintering at 1,000°C. The gelation of suspensions is attributed to the formation of hydrated compounds, later decomposed upon firing. Amorphous powders provided more intense gelation than semicrystalline ones, promoted a more homogeneous foaming, and stimulated a substantial crystallization upon firing. The homogeneity of foamed samples was assessed using micro-tomography and was further improved by casting foamed suspensions (“foam casting”) before setting.

Open Curettage With Bone Augmentation for Symptomatic Tumors and Tumor-like Lesions of Calcaneus: A Comparison of Bioactive Glass Versus Allogeneic Bone

Few studies have characterized the clinical outcomes of 45S5 Bioglass® applied as a bone graft to that of allogeneic bone applied in calcaneal open curettage. Therefore, the purpose of the present investigation was to compare the outcomes of patients with calcaneal tumors and tumor-like lesions treated by open curettage with 45S5 Bioglass® or allogeneic bone. Of the 31 patients who underwent open curettage (18 cases of unicameral bone cysts, 7 cases of aneurysmal bone cysts, and 6 cases of intraosseous lipoma), 16 (52%) received grafts with 45S5 Bioglass® and 15 (48%) with allogeneic bone. All the feet achieved bone fusion according to the modified Neer radiographic classification system at the last follow-up examination. The mean bone ingrowth time for the grafts with 45S5 Bioglass® versus allogeneic bone was 3.71 ± 0.86 versus 4.46 ± 1.04 months (p = .038), the mean bone healing time was 4.86 ± 0.93 versus 5.73 ± 1.07 months (p = .021), and the mean incision drying time was 7.2 ± 1.8 versus 8.2 ± 1.5 days (p = .047), respectively. No differences were found in the postoperative American Orthopaedic Foot and Ankle Society ankle-hindfoot scale scores between the 2 groups (p = .213). These results show that 45S5 Bioglass® can better facilitate the formation of new bone with a faster drying time of the incision than allogeneic bone. Although both materials can benefit the clinical outcomes of calcaneal tumors and tumor-like lesions, further studies are needed to observe the long-term complications and lesion recurrence rates.

Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue

Bioactive three-dimensional (3D) printed scaffolds are promising candidates for bone tissue engineering (BTE) applications. Here, we introduce a bioactive ink composed of Bioglass 45S5 (BG) and methacrylated collagen (CMA) for 3D printing of biomimetic constructs that resemble the organic and inorganic composition of native bone tissue. A uniform dispersion of BG particles within the collagen network improved stability and reduced swelling of collagen hydrogels. Rheological testing showed significant improvement in the yield stress and percent recovery of 3D printed constructs upon BG incorporation. Further, addition of BG improved the bone bioactivity of 3D printed constructs in stimulated body fluid. BG incorporated CMA (BG-CMA) constructs maintained high cell viability and enhanced alkaline phosphatase activity of human mesenchymal stem cells. In addition, cell-mediated calcium deposition was significantly higher on BG-CMA constructs, compared to CMA alone. In conclusion, 3D printed BG-CMA constructs have significant potential for use in BTE applications.

High solid content 45S5 Bioglass®-based scaffolds using stereolithographic ceramic manufacturing: process, structural and mechanical properties

45S5 Bioglass® has been widely used in bone tissue engineering scaffold due to its high bioactive properties. 3D printing method is a popular way to fabricate scaffolds. The extrusion printing method causes poor shape and dimensional accuracy of the scaffolds. The ordinary light-cured 3D printing method leads to low solid content of the scaffolds, which causes large shape shrinkages and low mechanical properties after sintering. In this study, we employed the stereolithographic ceramic manufacturing (SLCM) method to fabricate 45S5 Bioglass®-based scaffolds. Coating-then-stereolithographic layer by layer is the core process of SLCM method. Based on the optimization of the parameters of SLCM process and sintering process, the solid content of the 45S5 scaffold can be improved to a maximum of 70 %, and the linear shrinkage and compressive strength were effectively improved. The proposed method can be used not only for fabricating bone tissue engineering scaffold, but also for manufacturing other high solid content ceramic products.

Incorporation of Bioglass Improved the Mechanical Stability and Bioactivity of Alginate/Carboxymethyl Chitosan Hydrogel Wound Dressing.

Recently, hydrogel wound dressings were popular in wound healing because of their advantages over traditional gauze dressings. The alginate/carboxymethyl chitosan (SA/CMCS) hydrogel wound dressing has been widely studied because of its biocompatibility and antibacterial ability. However, the poor mechanical stability and lack of bioactivity limit their applications. Bioglass (BG) has been well acknowledged as a bioactive material, and SA/BG hydrogels have been reported to be able to promote wound healing. Calcium ions released from BG can further cross-link SA, which may enhance the mechanical stability of the SA/CMCS hydrogels. Therefore, in this study, BG was incorporated into SA/CMCS hydrogel in order to obtain a bioactive alginate/CMCS/BG (SA/CMCS/BG) hydrogel wound dressing with improved mechanical stability. Results showed that the Young's modulus of SA/CMCS/BG hydrogel was three times higher than that of SA/CMCS hydrogel. In addition to better antibacterial and coagulation properties, SA/CMCS/BG hydrogels possess stronger bioactivity than SA/CMCS hydrogels as they could accelerate skin wound closure by regulating the host inflammation responses, stimulating angiogenesis, and enhancing collagen deposition in wound sites, which suggests that SA/CMCS/BG hydrogels are good candidates for clinic wound dressings.

Bioactive glasses and electrospun composites that release cobalt to stimulate the HIF pathway for wound healing applications

BackgroundBioactive glasses are traditionally associated with bonding to bone through a hydroxycarbonate apatite (HCA) surface layer but the release of active ions is more important for bone regeneration. They are now being used to deliver ions for soft tissue applications, particularly wound healing. Cobalt is known to simulate hypoxia and provoke angiogenesis. The aim here was to develop new bioactive glass compositions designed to be scaffold materials to locally deliver pro-angiogenic cobalt ions, at a controlled rate, without forming an HCA layer, for wound healing applications.MethodsNew melt-derived bioactive glass compositions were designed that had the same network connectivity (mean number of bridging covalent bonds between silica tetrahedra), and therefore similar biodegradation rate, as the original 45S5 Bioglass. The amount of magnesium and cobalt in the glass was varied, with the aim of reducing or removing calcium and phosphate from the compositions. Electrospun poly(ε-caprolactone)/bioactive glass composites were also produced. Glasses were tested for ion release in dissolution studies and their influence on Hypoxia-Inducible Factor 1-alpha (HIF-1α) and expression of Vascular Endothelial Growth Factor (VEGF) from fibroblast cells was investigated.ResultsDissolution tests showed the silica rich layer differed depending on the amount of MgO in the glass, which influenced the delivery of cobalt. The electrospun composites delivered a more sustained ion release relative to glass particles alone. Exposing fibroblasts to conditioned media from these composites did not cause a detrimental effect on metabolic activity but glasses containing cobalt did stabilise HIF-1α and provoked a significantly higher expression of VEGF (not seen in Co-free controls).ConclusionsThe composite fibres containing new bioactive glass compositions delivered cobalt ions at a sustained rate, which could be mediated by the magnesium content of the glass. The dissolution products stabilised HIF-1α and provoked a significantly higher expression of VEGF, suggesting the composites activated the HIF pathway to stimulate angiogenesis.

Filament extrusion of bioresorbable PDLGA for additive manufacturing utilising diatom biosilica to inhibit process-induced thermal degradation

Bone scaffolds are often fabricated by initially producing custom-made filaments by twin-screw extruder and subsequently fabricating into 3D scaffolds using fused deposition modelling. This study aims to directly compare the effect of two alternative silica-rich filler materials on the thermo-mechanical properties of such scaffolds after extrusion and printing. Poly (DL-lactide-co-glycolide) (PDLGA) was blended with either 45S5 Bioglass (5 wt %) or Biosilica (1 and 5 wt%) isolated from Cyclotella meneghiniana a freshwater diatom were tested. Diatom-PDLGA was found to have similar mechanical strength and ductility to pure-PDLGA, whereas Bioglass-PDLGA was found induce a more brittle behaviour. Bioglass-PDLGA was also found to have the lowest toughness in terms of energy absorption to failure. The TGA results suggested that significant thermal degradation in both the Bioglass filaments and scaffolds had occurred as a result of processing. However, diatom biosilica was found to inhibit thermal degradation of the PDLGA. Furthermore, evidence suggested the agglomeration of Bioglass particles occurred during processing the Bioglass-PDLGA filaments. Overall, diatom biosilica was found to be a promising candidate as a bone filler additive in 3D printed PDLGA scaffolds, whereas Bioglass caused some potentially detrimental effects on performance.

Understanding the mixed alkali effect on the sinterability and in vitro performance of bioactive glasses

Bioglass 45S5 is widely known for its ability to regenerate bone. However, this glass cannot be fully densified by viscous flow due to its very high tendency for crystallization. In this work, we evaluate the sintering and bioactivity of Bioglass 45S5-based compositions in which Na2O is incrementally replaced by K2O. Our sintering tests demonstrated that the densification of Bioglass 45S5 powders can be significantly enhanced due to a decreased crystallization tendency. The composition in which half of the original Na2O content was substituted by K2O exhibits the highest densification rate, evidencing the mixed alkali effect (MAE) in the viscous-flow-driven sintering process. The replacement of Na2O by K2O significantly improved both the densification rate and the in vitro performance. These results are very relevant for this particular glass and also for the design of new bioactive glasses with improved sinterability.

Thermo-responsive injectable naringin-loaded hydrogel polymerised sodium alginate/bioglass delivery for articular cartilage

In the human body, joint cartilage is of great importance. It has long been a big therapeutic problem to fix joint cartilage lesions as it appears due to different conditions. Recent stories have shown that the cartilage replacement process must delay the extracellular (ECM) cartilage deterioration and modulate the host's inflammation response. For the reconstruction of the articular cartilage, drug-loaded injectable hydrogels were developed. This hydrogel could retain the chondrocyte phenotype, but the host's inflammatory reaction could also be controlled. The bioglass (BG)/sodium alginate (SA) injectable hydrogels was combined with agarose (AG)/Naringin hydrogel in injectable thermal response for articular cartilage regeneration with a non-chargeable hydrogel that contains both Naringin and BG (Naringin–BG hydrogels). The Naringin–BG hydrogel has an adequate swelling ratio that encourages the fusion of tissue formed with host tissue and enables the gradual release of Naringin bioavailabilities enhanced in situ. The Naringin–BG hydrogel can upgrade the typical chondrocyte phenotype by upregulating aggrecan, SRY-box 9, and collagen type II alpha one chain. It may also stimulate the polarization of M2 macrophage, lower inflammations, and prevent ECM degradations through the decrease of the expressions of the indictable metalloproteinase-13 matrix, nitric oxide synthase, and metalloproteinase-1 matrix. The formed tissues were identical to normal tissues and firmly incorporated with the surrounding tissue after administering the Naringin–BG hydrogels into the rat model articular cartilage defects. Then the injectable Naringin–BG hydrogel increases the bioavailable content of Naringin and retains the chondrocyte phenotype.

Titanium Dioxide Nanotubes Incorporated Bioactive Glass Nanocomposites: Synthesis, Characterization, Bioactivity Evaluation and Drug Loading

Nano bioactive glasses are known as suitable alternatives to repair the damaged bone tissues. In this research, novel sol-gel derived bioactive glass composites were synthesized through a reduction in the common weight percent of SiO2 substituted by 15 wt% of titanium dioxide nanotubes (TNTs) at two different steps by the synthetic procedure. The morphology, crystalline structure, and functional groups of the composites were evaluated through scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared (FTIR) analyses. Based on the SEM images, the step in which TNTs were added to the solution completely changed the morphology of the composite. Bioactivity tests were carried out by soaking the samples in the simulated body fluid (SBF) at the intervals ­of 14 and 28 days followed by the investigation of hydroxyapatite (HA) layer formation on the surface of the samples. According to XRD peaks at 2-theta angle of around 31 and 40 degrees, it was found that the presence of titanium dioxide nanotubes improved bioactivity after 14 days of immersion and both 58S-TNT composites were more bioactive than 58S bioglass, while 58S bioactive glass possessed more intense peaks of HA after 28 days of immersion in SBF. Furthermore, the drug loading characteristic of the prepared composites was examined and the results showed that the addition of nanotubes improved the drug loading performance of bioactive composites containing TNTs up to 70% compared to the 58S bioglass with 37% drug loading.

Enhancement of osteoporotic bone regeneration by strontium-substituted 45S5 bioglass via time-dependent modulation of autophagy and the Akt/mTOR signaling pathway.

Osteoporosis (OP) is a major systemic bone disease leading to an imbalance in bone homeostasis which remains a challenge in the current treatment of bone defects. Our previous studies on strontium (Sr) doping apparently stimulated osteogenesis of bioceramics, which suggested a promising strategy for the treatment of bone defects. However, the potential effects and the underlying mechanisms of Sr-doping on osteoporotic bone defects still remain unclear. Autophagy is a conventional self-degradation process of cells involved in bone homeostasis and regeneration under physiological and pathological conditions. Therefore, it is essential to design appropriate biomaterials and investigate the associated osteogenic mechanisms via autophagy. Based on this hypothesis, Sr-doped 45S5 bioglass (Sr/45S5) was fabricated, and ovariectomy bone marrow-derived mesenchymal stem cells (OVX-BMSCs) were applied as the in vitro cell culture model. First, the optimal Sr-doping concentration of 10 mol% was screened by cell proliferation, ALP staining, alizarin red S staining and the real-time PCR assay. Then, the results of western blot (WB) analysis showed that Sr-induced osteogenic differentiation of OVX-BMSCs was associated with time-dependent modulation of autophagy and related to the AKT/mTOR signaling pathway. Meanwhile, the autophagy in Sr-induced osteogenic differentiation of OVX-BMSCs was detected by WB, immunofluorescence staining and transmission electron microscopy. Furthermore, the effect of osteogenic differentiation of OVX-BMSCs has been significantly inhibited by the administration of autophagy inhibitors and the AKT/mTOR pathway inhibitors, respectively, in the early and late periods of osteogenic differentiation. Finally, the results of the model of femoral condyle defects in OVX-rats indicated that Sr10/45S5 granules remarkably enhanced bone regeneration which provided the evidences in vivo. Our research indicates that Sr-doping provides a promising strategy to promote osteogenic differentiation of OVX-BMSCs and bone regeneration in osteoporotic bone defects via early improvement of autophagy and late activation of the Akt/mTOR signaling pathway.

Self-crystallisation, an unexpected property of 45S5 Bioglass®.

Self-crystallisation of 45S5 Bioglass® powder and scaffolds was observed one year after their fabrication. Plate- and acicular-shape crystals, identified as calcium and sodium carbonates, grew at room temperature and atmospheric pressure, without any further treatment.

SYNTHESIS AND PRELIMINARY CHARACTERIZATION OF MODIFIED 45S5 BIOGLASSES

The study is devoted to synthesis and preliminary structural and morphological characterization of modified 45S5 glasses, in (66-x)SiO2∙27CaO∙4P2O5∙3TiO2∙xAl2O3 system, with potential applications in dentistry. The composition of the sol-gel derived samples was verified with respect to their nominal composition. The effect of partial SiO2 replacement with Al2O3 on samples structure and morphology is investigated. It was established that the Ca/P ratio is diminished in Al2O3 containing samples and that an amorphous hydroxyapatite phase similar to hydroxyapatite reported for bone tissue is formed.

Customized Borosilicate Bioglass Scaffolds With Excellent Biodegradation and Osteogenesis for Mandible Reconstruction.

Graft reconstruction of the mandible is an important approach that aims at improving the appearance and functionality of defected mandibles. The traditional implant materials are generally bioinert, non-degradable, and that they lack favorable pore structures for cell proliferation, which limit their clinical application. In this study, we used boron-containing bioactive glass which was combined with a three-dimensional (3D) printing technology to construct an osteoinductive implant scaffold, according to the imaging instructions of CT scan on bone defects. Here, the boron-containing bioglass scaffold (B-BGs) was prepared through sol-gel processing and a 3D print technique. Different boron content of borosilicate bioglass was prepared by incorporating B2O3 (molar: 19.4 and 38.8%) into 58S bioglass to replace parts of SiO2. For fabricated mandible implants through three-dimensional 3D printing of B-BGs (size: 8 × 2 mm; pore size: 250 μm) modified with borosilicate bioglass powder and sodium alginate. Notably, the compressive strength of the B-BGs was about 3.8 Mpa, which supported mandibular activity. Subsequently, the excellent biocompatibility of B-BGs was confirmed using cytotoxicity in vitro studies. Finally, data from in vivo experiments demonstrated that the B-BGs could promote bone regeneration and they could almost get completely degraded within 4 weeks. Our results showed that the boron-containing bioglass could repair mandibular defects.