10th Day Research Articles

The effect of thiocetam on the parameters of the nitric oxide system under the conditions of the experimental periodontitis and immobilization stress formation

Introduction and aim. The aim of this work is to study the parameters of the nitric oxide (NO) system in the blood of guinea pigs under the conditions of the experimental periodontitis (EP) and immobilization stress (IS) formation and to evaluate the effectiveness of thiocetam use. Material and methods. Experimental studies were performed on 50 guinea pigs (males, body weight 0.18–0.21 kg) which were divided into five groups (10 in each): the first group were intact animals as control; the second experimental group were animals with experimental periodontitis under conditions of immobilization stress (3rd day), the third group included guinea pigs with EP and IS on the 5th day of the combined model process, group IV – animals with EP and IS 15th day (without administration of thiocetam) and group V – animals on the 15th day of experiment with EP and IS after use of thiocetam. Results. As a result of this research, changes in the activity of the NO system in the blood were observed, namely an increase in the level of stable metabolites and an increase in the activity of total NO-synthase, which is accompanied by a compensatory inhibition of the L-arginine activity, and these indicators were most pronounced in the late stages of EP and IS formation. Conclusion. The use of thiocetam showed a corrective effect on the changed variables of NO metabolism in the peripheral blood of guinea pigs under the conditions of the EP and IS development.

Histological analysis of Macrobrachium rosenbergii (de Man) eggs incubated in different salinities

Macrobrachium rosenbergii is the freshwater shrimp species of greatest economic interest, and its reproduction has been studied for commercial purposes. This study aimed to evaluate the effect of different salinities (0, 6, and 12 mg L-1) on the embryogenesis of this species. The females were observed daily according to their reproductive behavior. Upon the first sign of spawning, when their eggs were attached to the abdomen, they were collected for egg collection and transferred to the incubators at the respectively tested salinities. The eggs were collected every five days for up to 23 days, separated according to their stage of development, histologically processed, and stained for visualization of structural changes during embryogenesis using an optical microscope. From the egg's histological images at different embryonic development stages, the egg hatching time was evaluated, the changes that occurred in the eggs during embryogenesis, the total area of the eggs, the vacuole area, and the ratio between the vacuole area and the total area of the eggs. Differences were observed in hatching time, egg size, and vacuole area and area ratio. The 6 g L-1 of salinity treatment was statistically different from the other in all parameters analyzed. Therefore, it was concluded that 6 g L-1 of salinity positively influenced embryogenesis, as it presented hatches from the 15th day of embryogenesis, demonstrating that there was a greater speed of migration of vitellogenin for the formation of the embryo, accelerating embryogenesis and hatching of M. rosenbergii.

무성서원 院規의 유래와 내용상의 특징

In this paper, the origin and characteristics of the academic regulations of Museong Seowon, which are currently included in the 1884 and 1936 edition of the Seowon historical Book, were examined. According to this, the academic regulations of Museong Seowon, which is currently handed down, was made based on “Seoksil Seowon Hakkyu” by Kim Won-haeng (1702~1772). Accordingly, in this paper, I first examined why the academic regulations of Museong Seowon was established with Kim Won-haeng's “Seoksil Seowon Hakkyu” as the original. In this regard, this paper paid attention to the fact that Kim Won-haeng was appointed as the third director of Pilam Seowon in a similar location in the same Jeolla-do area in 1762 (Yeongjo 38). Regarding the characteristics of the contents of the academic regulations of Museong Seowon, this paper reviewed two main points. First, I examined how Toegye Yi Hwang (1501~1570)'s “Isanwongyu” clause 6, which says, “Books cannot go out of the gate of Seowon,” was adopted as a rule of Museong Seowon, and I examined how important and difficult the management of books of Museong Seowon had been at that time. Second, I reviewed the reasons why articles 2 and 21 among Seoksil Seowon Hakkyu, which are provisions on the appointment of the director and reading activities on the academic regulations held on the 1st and 15th day of every month were not adopted as the academic regulations of Museong Seowon, even though they were the basic provisions for the education and operation of Seowon. According to the paper's consideration, the reason why the two provisions of Seoksil Seowon Hakkyu were not adopted as the academic regulations in Museong Seowon seems to be because Museong Seowon was in such a difficult situation that there was no director until 1871 and the memorial service for spring and fall was not properly held. On the other hand, it can be interpreted as evidence that the academic regulations of Museong Seowon were not simply followed by Seoksil Seowon Hakkyu, but were made based on a cool self-awareness of the reality of the Museong Seowon.

Portulaca oleracea Mitigates 3-Nitropropionic Acid-induced Motor Impairment, Mitochondrial Dysfunction and Free Radical Generation in Male Wistar Rats

The mitochondrial toxin 3-nitropropionic acid (3-NP) effectively induces behavioral changes and selective striatal lesions in rats mimicking clinical Huntington’s disease (HD). In the present study, behavioral tests and biochemical analysis were performed to explore the role of ethanolic extract of Portulaca oleracea (POEE) in the experimental animals of HD. Male wistar rats received an intraperitoneal injection of 3-NP (10 mg/kg) for 14 days. Simultaneously in treatment groups, the animals received POEE at a dose of 2 and 4 mg/kg along with 3-NP injection for a period of 14 days. The motor behavioral assessments were performed on days 5, 10 and 15. After the behavioral test on 15th day, the biochemical and histopathological parameters were performed. Administration of 3-NP resulted in body weight change, motor and memory impairments. 3-NP administration also causes marked oxidative stress by showing significantly increased levels of lipid peroxidation (LPO), nitric oxide (NO) and reduced activities of endogenous antioxidants. Further, it damages the mitochondrial complex enzyme succinate dehydrogenase (SDH) and elevated the acetylcholine esterase (AChE) activity. Treatment with POEE at 2 and 4 mg/kg bw doses significantly restored the body weight changes and behavioral impairment in 3-NP induced rats. Further, POEE significantly decreased the oxidative damages, restored the antioxidant enzymes activities and mitochondrial dysfunction. Histopathological sections of the striatal region showed the extent of neuronal loss in 3-NP induced rats and was restored upon POEE treatment. From the results, we suggest that POEE might be effective in clinical HD by virtue of its antioxidant and neuroprotective properties.

Development of Coagulopathy and Pancreatitis Due to Saw Palmetto Used for Urinary Symptoms

Aim: Serenoa repens, commonly known as saw palmetto, is a widely used herbal supplement for lower urinary tract symptoms. However, data regarding the toxicity of this dietary supplement is limited. In this study, it is aimed to emphasize that herbal extracts such as saw palmetto can cause coagulation disorder and pancreatitis. Case Presentation: In this case report, we present a 60 year-old male patient using saw palmetto extract for urinary stone disease and urinary tract symptoms. The patient had widespread ecchymoses on the tip of the tongue, trunk and extremities. The coagulometric tests of the patient, who did not use any anticoagulant drugs, were prolonged; renal failure had also developed. Vitamin K and fresh frozen plasma were used to treat the coagulopathy. The patient, who also developed acute pancreatitis in the days following hospitalization, was discharged on the 15th day without any additional complications. Conclusion: Herbal supplements are widely used in medical practice. Saw palmetto extracts may have different adverse effects including abdominal pain, headache, hepatotoxicity, coagulopathy, and pancreatitis. Patients using these supplements should be aware of these effects of saw palmetto.

Pengaruh penambahan Lactobacillus plantarum dalam pembuatan mie kering berbahan rambut jagung manis (Zea mays var. Saccharata)

Rambut jagung manis is one of the agricultural waste products that is still minimally processed. One form of processed rambut jagung manis as a basic ingredient for making dry noodles. However, the resulting noodle product has a weakness in the form of a short shelf life due to the presence of spoilage fungi such as mold. Therefore, efforts need to be made to reduce the amount of mold by adding Lactic Acid Bacteria (L. plantarum). The method used in this research was an experimental method with a completely randomized factorial design (RALF), namely factor I L. plantarum concentration (0%, 2%, 4%, 6% (v/v)) and factor II storage time (0 days, 5 days, and 10 days) with 3 repetitions, so that 36 experimental units were obtained. The parameters measured in this study were the degree of acidity (pH), mold numbers, and nutritional value. The results showed that the average mold number at T0 was 3.40 ± 0.109 in dry noodles made from corn silk which were fermented without adding L. plantarum. Meanwhile at concentrations of 2%, 4% and 6% respectively as follows 3.24 ± 0.174; 3.30±0.240; and 3.34 ± 0.000. Mold content (Aspergillus sp.). Nutrient content tests after storage on the 10th day (T2) in L. plantarum concentration treatments of 0%, 2%, 4%, and 6% showed protein levels of 16.05%; 18.13%; 20.04%; and 20.36% bw and glucose levels respectively 78.87%; 83.16%; 81.56%; and 78.84%. The numerical value of yeast mold population in mie from rambut jagung during storage can be reduced and the protein content increases so that the addition of L. plantarum can be used as food biopreservative.

A data-driven analysis on the mediation effect of compartment models between control measures and COVID-19 epidemics

By collecting various control policies taken by 127 countries/territories during the first wave of COVID-19 pandemic until July 2nd, 2020, we evaluate their impacts on the epidemic dynamics quantitatively through a combination of the multiple linear regression, neural-network-based nonlinear regression and sensitivity analysis. Remarkable differences in the public health policies are observed across these countries, which affect the spreading rate and infected population size to a great extent. Several key dynamical features, like the normalized cumulative numbers of confirmed/cured/death cases on the 100th day and the half time, show statistically significant linear correlations with the control measures, which thereby confirms their dramatic impacts. Most importantly, we perform the mediation analysis on the SEIR-QD model, a representative of general compartment models, by using the structure equation modeling for multiple mediators operating in parallel. This, to the best of our knowledge, is the first of its kind in the field of epidemiology. The infection rate and the protection rate of the SEIR-QD model are confirmed to exhibit a statistically significant mediation effect between the control measures and dynamical features of epidemics. The mediation effect along the pathway from control measures in Category 2 to four dynamical features through the infection rate, highlights the crucial role of nucleic acid testing and suspected cases tracing in containing the spread of the epidemic. Our data-driven analysis offers a deeper insight into the inherent correlations between the effectiveness of public health policies and the dynamic features of COVID-19 epidemics.

Study of Influence of Various Methods of Intraoperative Keratoprotection on Morphological and Functional State of Ocular Surface System in Early Period after Phacoemulsification in Senile Cataract

Purpose: to study the effect of various methods of intraoperative keratoprotection on morphological and functional state of ocularsurface system (OSS) in early period after phacoemulsification (PE) in senile cataract (SC). Patients and methods. 240 patients (240 eyes) who applied for surgical treatment of SC. Age: 65 ± 4 years. 109 men, 131 women. All patients were divided into three observation groups according to the method of intraoperative keratoprotection: 1st group — 60 patients, corneal irrigation with balanced solution; 2nd group — 60 patients, ophthalmic viscosurgical device was applied to the cornea; 3rd group: 3a subgroup — 60 patients, soft contact lens (SCL) was applied to the cornea; subgroup 3b — 60 patients, SCL impregnated with riboflavin solution was applied to the cornea. In addition to the standard ophthalmological examination, Schirmer I test, OCT-scanning were performed, and tear breakup time (TBUT) was determined. Results. Operations were performed without complications. On the 1st day after the operation, the parameters of the Schirmer I test were higher in the eyes of 1st and 2nd groups. On the 5th day after the operation, tear production decreased sharply in all eyes. On the 10th day, an increase in tear production was observed in all groups, the maximum increase — in 3b subgroup. On the 1st day after the operation, TBUT decreased in all examined eyes. The largest significant TBUT decrease was noted in 1st group, the smallest — in 3b subgroup. On 1st day, corneal epithelial thickness (CET) decreased in all eyes, the maximum decrease was detected in 1st group. On the 5th day, CET continued to decrease in all eyes, the minimum decrease occurred in the 3b subgroup. On the 10th day — a decrease in CET in all groups with minimal significant dynamics in 3b subgroup. Conclusion. The study showed that the state of OSS in the early period after PE in SC depends on the method of intraoperative keratoprotection. The most optimal method of intraoperative protection of corneal epithelium and prevention of negative morphological and functional state of OSS after PE in SC is using of SCL impregnated with riboflavin.

Hydroxyproline as a Marker of the Efficiency of Skin Wound Therapy in Ischemic Conditions

The aim of the study was to analyze the level of hydroxysoplin in tissues as a marker of healing efficiency of experimental skin wound under ischemia conditions. A wound was reproduced according to our developed method on 90 rats Vistar. There were 3 groups of rats of 30 individuals. Therapy was not carried out in the group «Control», local treatment with multi-component means was performed in the «First experimental» group, magnetotherapy was performed with the above-mentioned agent in the «Second experimental» group. The hydroxylol content was estimated based on Newman’s, Logan’s technique modified by Cissel. The results were compared with planimetric, visual and morphological data. The formation of scar was observed in each series on the 10th day, there was recorded the predominance of mature connective tissue over granulation in the experimental groups in contrast to the «Control» group. There was a strong correlation between the hydroxyprolin content and the wound area in all groups. The article marked the reliability of the method of quantitative analysis of hydroxyproline, which made it possible to increase the objectivity of the representation of the course of the wound process in conjunction with other methods of research.

Effect of selected environmental factors on the growth and survivability of the larvae of the Chinese sleeper Perccottus glenii Dybowski 1877

The aim of this study was to determine the influence of the temperature (range 15 to 25°C), pH (6 to 9) and salinity of water (between 0 to 10‰) on the growth, condition and survival of larvae of the Chinese sleeper. During the 28‐day experimental rearing, the highest average body weight of 81.3 ± 16.3 mg was reached in fish from the group maintained at a water temperature 25°C, and was almost five times higher than the weight at the temperature of 15°C (15.6 ± 2.2 mg). A significantly higher body weight of Chinese sleeper larvae was also recorded in the group reared in alkaline water (pH 9), compared to those kept in acidic and neutral water. Statistically significant differences between the groups (p≤0.05) were recorded for the condition index (K) values and relative body weight (SGR) only in the experiment with different water temperatures of fish rearing. The highest number (16% of the initial stock) of abnormal larvae, associated with a lower jaw and vertebral structure deformities, was recorded in the group reared at 5‰ salinity (Z5). Simultaneously, 100% mortality of the stock was noted by the 10th day of rearing in the 10‰ salinity. The cumulative mortality of the Chinese sleeper larvae related to cannibalism Types I and II in all the experimental rearing was low, oscillating between 0.5% and 11.5% in the groups bred in water with a pH 9 and 0‰ salinity, respectively. The study results may help determine the critical values of water parameters that promote or limit development in the early stages of the Chinese sleeper and could be used in activities related to eradicating or significantly reducing the increasing abundance of this alien invasive fish species in freshwater ecosystems.

Potential protective effect of alpha lipoic acid against testicular oxidative stress and altered gene expression induced by gentamicin treatment

Background: Gentamicin induces testicular damage in association with oxidative stress explained by different mechanisms, including gene expression suppression of some antioxidant enzymes. The aim of the study: To investigate the potential protective effects of α-lipoic acid against gentamicininduced testicular oxidative stress in terms of altered gene expression. Materials and methods: A parallel experimental study was conducted including fifty adult male albino rats. The animals were grouped into five. The control group received intraperitoneal NaCl 0.9%, while the other groups received 36.5mg/kg/day intraperitoneal gentamicin. Group-1 received gentamicin only, group-2 received gentamicin + intraperitoneal α-lipoic acid 100mg/kg/day, group-3 received gentamicin + intraperitoneal α-lipoic acid 200mg/kg/day, and group-4 received gentamicin + oral vitamin E 100mg/kg/day. All treatments were given for 14 days. The animals were euthanized in two halves on the 15th and 60th days. Testes were immediately removed, frozen, and evaluated for oxidative stress biomarkers and gene expression of antioxidant enzymes. Results: Gentamicin increased malondialdehyde by mean difference ± standard error of 115.57 ± 2.18, decreased total antioxidant capacity by -9.23 ± 0.27, and decreased catalase and superoxide dismutase activities by -1.89 ± 0.45 and -11.77 ± 2.86, respectively, compared to the control. Additionally, gentamicin downregulated gene expression of catalase, glutathione peroxidase, and superoxide dismutase. However, adding vitamin E or α-lipoic acid cured the oxidative stress and partially to completely upregulated the gene expression. Conclusion: Reversal of testicular oxidative and gene suppression associated with gentamicin could be achieved with α-lipoic acid co-treatment, which might be applied in human studies.

Tissue distribution and residue depletion of difloxacin in crucian carp (Carassius carassius) after multiple oral administration

Although difloxacin is not listed by Codex or the US FDA, China and some countries in Asia have approved this drug for use in aquaculture. Additionally, both China and EMA have adopted the same maximum residue limit (MRL) for difloxacin in muscle plus skin at 0.3 μg/g. However, a withdrawal period has not been established for difloxacin in aquaculture. Therefore, the primary goal of this investigation was to evaluate the tissue distribution and residue depletion of difloxacin in crucian carp (Carassius carassius) kept in freshwater at a temperature of 21 ± 1 °C, with the aim of determining an appropriate withdrawal period. This investigation entailed the oral administration of difloxacin via gavage, employing a daily dosage of 20 mg/kg body weight (BW) over a consecutive three-day regimen. Diverse samples, including plasma, bile, skin-on-muscle, liver, kidney, intestine, and gill, were collected at predetermined time intervals after the final administration. The quantification of difloxacin concentrations in all collected samples was carried out utilizing a high-performance liquid chromatography (HPLC) method. Difloxacin exhibited extensive distribution within crucian carp, with detectable presence in all examined tissues. The highest average concentration was notably observed in the bile with a Cmax of 14.359 μg/mL. Remarkably, even on the 15th day (360 h) following the final dosing, traces of difloxacin residues remained detectable in all collected samples. Pharmacokinetic parameters, determined through the noncompartmental model, unveiled diverse elimination half-lives (t1/2λzs) for difloxacin across distinct samples, ranging from rapid to slow: plasma (9.46 days), skin-on muscle (10.15 days), liver (10.36 days), bile (13.05 days), intestine (20.94 days), kidney (22.12 days), and gill (29.56 days). Taking into consideration the MRL, a withdrawal period of 32 days was definitively determined, derived from the upper limit of the one-sided 95% confidence interval. When converted to the unit of °C-day, the withdrawal period was determined to be 660.

The typical developmental trajectory and energy requirements of Shitou goose during the embryonic stage

Low hatchability has been a persistent challenge in the goose industry. Establishing standard atlases and comprehending embryonic development patterns are essential to improving the hatching rates of goose eggs. However, comprehensive descriptions of normal atlases, embryonic development, and energy requirements in geese are lacking. In this study, a total of 120 fertile eggs from well-known large Shitou goose were incubated using 12 nesting purebred female geese. During hatching, both the temperature of the eggshells and the weight of eggs were recorded, and daily photographs of the embryos were captured to monitor their development closely. After hatching, count the number of pores per unit area by choosing eggs without sperm, dead embryos, or abnormal shells. Furthermore, 150 Shitou goose eggs were hatched by automatic incubator, with adjustments made based on observed normal developmental stages that incubated by female geese. The eggs were carefully opened to meticulously document embryonic morphology and create a detailed development map. Measurements were taken of the eye diameter, length of the lower beak, tarsometatarsus bone, and embryo length. Subsequently, an analysis was conducted to assess the calcium, phosphorus, crude protein, and crude fat content to study the energy requirements for embryo development. As a result, the hatch rate of the incubator reached 86.67%, and the cumulative water loss rate increased with embryo age. Notably, normally developing embryos displayed a significantly higher number of pores on the eggshell surface compared to dead embryos (P < 0.05). Additionally, embryonic body length, eyeball diameter, and lower beak length exhibited continuous growth until day 19 of incubation, while tarsometatarsus length increased steadily from days 12 to 31. Liver size measurement began on the 10th day of incubation, while both leg and chest muscles showed continuous growth from the 12th day. For energy demand, the embryo primarily relied on protein sourced from the egg yolk within the first 10 days of development. Afterward, the egg yolk provided both protein and fat for embryonic growth. In summary, this study has generated a comprehensive developmental map for Shitou goose embryos, offering valuable insights into their growth and morphological changes throughout the incubation period. This map can serve as a reference for optimizing machine incubation techniques to enhance goose egg hatching rates and provide fresh perspectives on the development of geese.

ECONOMIC AND BIOLOGICAL EVALUATION OF RADISH HYBRIDS FOR GROWING IN OPEN SOIL IN THE CONDITIONS OF THE CENTRAL PART OF THE RIGHT BANK FOREST-STEPPE

Radish is an early vegetable crop with a very short growing season, widely cultivated worldwide. At the same time, the extensive varietal resources of radishes and the changing environmental conditions compel agroproducers to perform more careful selection of varieties/hybrids to achieve high yields of marketable products. For this purpose, an agro-biological evaluation of eight radish hybrids of different maturity periods was conducted in open-field cultivation. Differences were found in both the duration of phenophases and the growing period of radish plants. The hybrids Stellar and Rockstar were characterized by faster entry into phenological phases, root formation, and maturation, reaching the technical maturity phase on the 25th day. In terms of biometric indicators at the technical maturity phase (BBCH 49), the hybrid Adele stood out, with plants having the highest height (18 cm), leaf rosette width (18 cm), number of leaves per plant (8 pcs), and area (17.6 thousand m2/ha). Over the years of research from 2017 to 2019, which were marked by contrasting weather conditions, the highest yield was obtained from the Adele hybrid, at 26.1–27.3 t/ha, with a 15% advantage over the control and a yield increase of 3.5 t/ha. The hybrids were ranked by yield as follows: Adele &gt; Eliza &gt; Stellar &gt; Rockstar &gt; Rosetta &gt; Roxanne &gt; Donar &gt; Rolex. The highest quality indicators were found in the root crops of the Adele, Stellar, and Rockstar hybrids. Based on the visual assessment of radish root crops, analysis of their linear dimensions, and quality indicators, the hybrids were ranked as follows: Adele &gt; Stellar &gt; Rockstar &gt; Eliza &gt; Rosetta &gt; Roxanne &gt; Donar &gt; Rolex. A strong direct correlation was found between yield and root weight (r=1), as well as between the developmental phases of the plants (seedling emergence, appearance of the first true leaf, growth and development of leaves and roots) and the technical maturity of the root crops (r=0.90–1.00). The number of leaves per plant correlated with plant height (0.97) and the developmental phases of the plants (0.91–0.97). The sugar content in the root crop correlated with the dry matter content and the root diameter (0.92). Based on the agro-biological evaluation of radish hybrids of different maturity groups, it was determined that the most productive hybrids with quality root crops for open-field cultivation are the early-maturing Stellar, the mid-maturing Eliza, and the late-maturing Adele, which are recommended for cultivation in farms of various ownership forms in the central part of the Right-Bank Forest-Steppe.

ANALYSIS OF ANTIOXIDANT SYSTEM OF TEST ANIMALS IN THE DYNAMICS OF THE WOUND PROCESS AT LOCAL APPLICATION OF HYDROGEL DRESSINGS

Introduction. The development of the inflammatory process is always accompanied by the activation of lipid peroxidation, which is caused by tissue hypoxia. This, in turn, catalyzes a change in the physiological parameters of the antioxidant system. The aim of the study is to analyze the dynamics of the antioxidant balance of wounds in an experiment using hydrogels saturated with silver ions and an antioxidant drug for the local treatment of inflammatory processes. Object and research methods. The experimental study was carried out on 80 Wistar rats weighing 150 to 200 grams. Animals were removed from the experiment. for 3, 7, 10, 14 days. Catalase activity in the dynamics of the wound process was determined. Research results and their discussion. The content of catalase on the 7th day in the third experimental group is 0.51±0.03 μKat/l. On the 10th day, the indicator of the antioxidant system in the second (0.44±0.05 μKat/l) and third experimental groups was 0.42±0.02 μKat/l. At the final stage of the study on the 14th day, the analysis of catalase content showed that in all animals with a simulated inflammatory process, the indicator of antioxidant activity had no significant differences between themselves and, importantly, with the indicators of intact animals (0.33±0.01 μKat /l). The indicators of the control (0.33±0.04 μKat/l) and experimental groups were as follows: the first experimental group – 0.32±0.06, the second experimental group – 0.36±0.04, the third experimental group – 0, 38±0.06 μKat/l. Antioxidant protection was supplemented by local release of a drug with antioxidant action from the hydrogels, which contributed to a reduction in the intensity of free radical oxidation processes. That is, in almost all groups of animals, which were simulated purulent-inflammatory wounds, indicators of catalase activity approached the level of intact animals already on the 14th day. Conclusions. Application for local treatment of hydrogel bandages saturated with an antioxidant drug, which is released into the wound surface for a long time, promotes the activation of antioxidant protection and suppresses the processes of free radical formation.

Successful Treatment of Olecranon Fracture with Tie-in External Fixator in a Cat

In this case report, A 4-months-old, male, British shorthair cat was presented with lameness and swelling in the forelimb and loss of appetite after a fall from a height. On the orthopedic examination, pain, lameness, abnormal mobility, local sensitivity, crepitation, and deformation findings were observed in the forelimb. Clinical and radiological examinations revealed an avulsion fracture of the left olecranon. The fracture was treated with an external fixator. Clinical and radiological controls were performed on the 10th, 21st, 30th, and 45th postoperative days. The external fixator was removed on the 62nd postoperative day under sedation. This case report aimed to contribute to the literature on the successful treatment of fracture of the olecranon with an external fixator in a 4-months-old young cat.

Utilization of Livestock Manure as Biogas and Liquid Organic Fertilizer (LOF) in Sumbermulyo Village, Pesanggaran, Banyuwangi

Biogas is a natural gas produced through anaerobic bacteria breakdown of organic materials. Organic materials can include livestock manure, domestic waste, and other organic waste that bacteria can decomposeunder anaerobic conditions. Sumbermulyo is one of the villages in the Pesanggaran sub-district of Banyuwangi Regency, where the majority of the population works as dragon fruit farmers and goat herders. The current phenomena in the area include the scarcity of subsidized urea fertilizer and Liquified Petroleum Gas (LPG). An alternative approach is to utilize goat manure as a source of methane gas, which can be used as a substitute for LPG. The objective of this community service project is to establish a biogas processing pilot project in the Sumbermulyo Village, Pesanggaran sub-district, which the community can implement. Additionally, it aims to analyze the total N, P, K content in the wet sludge from the digester as liquid organic fertilizer. The method used involves constructing a biogas digester on the land owned by one of the farmer groups and evaluating the gas production. The digester is built using a concrete model (fixed dome) in the shape of an airtight dome. The construction of the digester takes 14 days until it is ready for use. Up to the 10th day of use, thehighest pressure is obtained on day -10, with a pressure of 107,486.76 N/m2 or 1.06 atm. Chemical analysis of the liquid organic fertilizer shows that the total N, P, K content is as follows: 0.032%, 0.009%, 0.144% for the sample from the test digester, and 0.044%, 0.005%, and 0.195% for the sample from the reference digester

Studying the effectiveness of Jatropha carcus L. Extract as a repellent, antifeedant, and toxic substance against red palm weevil (Rhynchophorus Ferrugineus) adult insects in Saudi Arabia

ObjectiveRed palm weevil (RPW), Rhynchophorus ferrugineus, is a highly harmful pest that causes severe damage to date palm trees. The detrimental influence of RPW on date palm trees cannot be ignored and must be addressed. Chemical insecticides are available for controlling RPW; however, the insect population has become resistant to these chemicals, leading to damage and spread to new areas. The present investigation examined the impacts of physic nut (Jatropha curcas L.) seeds essential oils (PNEO) on laboratory-reared RPWs adult insects reared in a controlled laboratory environment. MethodsThe insecticidal, repellent, and antifeedant potentials of different doses (10, 20, 30, 40, 50%) of PNEO were tested against RPW. The insecticidal activity of J. curcas seeds oil extracts was assessed using topical application and feeding methods. ResultsRPW showed a high mortality rate when J. curcas seeds oil was applied topically after 24 h (P < 0.05). The mortality (%) of insects was 8, 76, 92, 96, and 100 for 10 %, 20 %, 30 %, 40 %, and 50 % concentrations, respectively. The LC50 and LC90 were 16.50 %, and 28.14 %, respectively. Results from the feeding method demonstrated a significant increase in mortality with a 100 % mortality rate at higher concentrations of PNEO compared to 10 and 20 % concentrations (P < 0.0001). The LC50 and LC90 were 24.42 % and 31.30 %, respectively. There was no significant decrease in food consumption and the antifeedant index when 10 % and 20 % of J. curcas seeds oil were used on the 3rd and 6th day after application. All J. curcas oil concentrations showed a repellent effect significantly higher than the controls (P < 0.05). ConclusionJ. curcas seeds oil has shown potential insecticidal and repellent effects against RPW, and it could provide an eco-friendly tool for RPW pest management.

Elicitor-Mediated Enhancement of α-Tocopherol in Cell Suspension Cultures of Nicotiana tabacum

Plant cell and tissue culture have been used as the alternative and potential renewable source for the production of valuable phytochemicals. Elicitation offers a reliable in vitro approach to produce or enhance potential phytochemicals. α-tocopherol, which is an isoform of vitamin E, is a potent fat-soluble phytochemical known in nature. The present study focused on enhancing the production of α-tocopherol in the cell suspension culture through an elicitation approach. Suspension cultures of Nicotiana tabacum were established from the leaf disk-derived callus. The cell suspension cultures were treated with different elicitors (methyl jasmonate, salicylic acid, and yeast extract) at the lag phase of the cell growth cycle. The effects of elicitors on cell cultures were determined in terms of biomass, and α-tocopherol enhancement was determined using high-performance liquid chromatography (HPLC). Different elicitors depending on the concentration exerted different effects on cell growth and α-tocopherol production. Methyl jasmonate treatment showed the significantly highest increase in α-tocopherol on the 6th day of treatment in tobacco suspension cultures. Methyl jasmonate at the concentration of 150 μM enhanced α-tocopherol content to 24-fold over the control. This study clearly shows that the elicitors had the potential to increase the accumulation of α-tocopherol considerably in tobacco cell cultures. The outcomes of this study could be of considerable importance to the nutraceutical and pharmaceutical industries.

Multifunctional sprayable carboxymethyl chitosan/polyphenol hydrogel for wound healing

The use of facile methods to synthesize environmentally friendly and multifunctional hydrogel dressings is still a major challenge in development. Herein, Turkish gall extract (TGE) and carboxymethyl chitosan (CMCS) were combined and sprayed using a dual syringe to form a multifunctional TGE-CMCS hydrogel (TC gel) in one step through abundant hydrogen bonding between functional groups as a green approach. TC gel showed rapid gelation at 19.0 ± 2.9 s. Apart from the advantage of being able to adapt to different wound shapes, TC gel retained the antioxidant, antibacterial, hemostatic and anti-inflammatory properties of TGE. In vitro antibacterial experiments showed that TC-gel eliminated 98.27 ± 0.79 % of Staphylococcus aureus and 98.87 ± 1.08 % of Escherichia coli. Compared with TGE or CMCS alone, TC gel accelerates skin wound healing due to its three-dimensional network structure and continuous release of active components at the wound site, enhancing re-epithelialization, improving collagen deposition, and increasing angiogenesis. The wound healing rate of full-thickness skin defect rats treated with TC gel was 93.98 ± 0.63 % on the 10th day. These results suggest that TC gel combined with a facile and scalable manufacturing method is a promising multifunctional wound dressing for clinical wound management.