This study explores the possibility of estimation of the sensible surface heat flux using satellite-derived surface temperature and road pavement temperature together with in-situ wind and air temperature measurements by the profile method. A 10-year series of data from the roadside weather observation network was used. This dataset contained wind (measured at 5.8–9.5 m above ground) and air temperature (measured at 2.6–4.8 m) together with road surface temperature. Another dataset consisted of 254 simultaneous MODIS observations. A high correlation (0.94) of the surface temperature measured by both methods was noted despite coarse pixel size. We considered satellite-derived surface temperature to determine the sensible heat flux by the profile method; these results were compared to the values obtained using road temperature measured by pavement-mounted sensors. While the overall correlation is relatively strong (0.70) and considerable systematic differences exist, the values of heat flux calculated at different locations show a high spatial coherence - either when using the in situ pavement temperature (correlation ranging from 0.84 to 0.94 for daytime and 0.63–0.84 for nighttime) or the satellite-derived temperature (correlation coefficient 0.72). In most cases, differences between the two flux estimates can be linked to local factors such as the land use structure.