Abstract
Wu Shan Shen Cha is the leaf of Malus asiatica Nakai., a special type of tea that is consumed in the same way as green tea. To study the effect of Wu Shan Shen Cha-derived flavonoids (WSSCF) on lesions in the stomach, a 15% hydrochloric acid–95% ethanol (volume ratio 4:6) solution was used to induce gastric injury in mice. The degree of gastric injury was assessed using tissue specimens, and the effects of WSSCF on the serum levels of antioxidant enzymes were investigated. The results showed that WSSCF could alleviate the damage of the gastric mucosa and gastric wall caused by the hydrochloric acid–ethanol solution, decrease the tissue and serum levels of malondialdehyde (MDA) in mice with gastric injury, and increase the serum levels of superoxide dismutase (SOD) and glutathione (GSH). The results of quantitative polymerase chain reaction (qPCR) showed that WSSCF could increase the mRNA expression of Mn-SOD, Cu/Zn-SOD, catalase (CAT), endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) in tissue specimens from mice with gastric injury and decrease the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). At the same time, the results of the high concentration of WSSCF (WSSCFH) group were closer to those of the drug (ranitidine) treatment group. Wu Shan Shen Cha-derived flavonoids had a good antioxidant effect, so as to play a preventive role in alcoholic gastric injury.
Highlights
Alcohol consumption by humans is on the rise, and the incidence of digestive tract diseases in the drinking population is significantly higher than that in the non-drinking population [1]
The gastric lesions in mice of the ranitidine group were the least severe, and the inhibition rate of gastric injury was the highest. These results indicate that lesions in mice of the WSSCFH group were similar to those in mice of the ranitidine group, suggesting that Wu Shan Shen Cha-derived flavonoids (WSSCF) can alleviate alcohol-induced gastric injury
The protective effects of flavonoid extracted from Wu Shan Shen Cha (WSSCF) on alcohol-induced gastric injury in mice were evaluated
Summary
Alcohol (ethanol) consumption by humans is on the rise, and the incidence of digestive tract diseases in the drinking population is significantly higher than that in the non-drinking population [1]. The levels of lipid peroxides and free radicals in the gastric mucosa of alcoholic drinkers were elevated. Biomolecules 2019, 9, 169 free radicals acting on mitochondria can reduce ATP production, cause Na+-K+-ATPase dysfunction, increase the intracellular Na+ concentration, trigger Na+-Ca2+ exchange, and increase intracellular calcium. Reactive oxygen species can directly inhibit Ca2+-Mg2+-ATPase, suggesting that oxygen free radicals may trigger calcium overload [4]. The damage of the gastric mucosa caused by ethanol may be related to the decreased fluidity of cell membranes caused by excessive oxygen free radicals and intracellular calcium overload [5]
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