Abstract
ABSTRACTHere, we present the draft whole-genome sequence of Vibrio cholerae O1 El Tor strains 76 and M3265/80, isolated in Mariupol, Ukraine, and Moscow, Russia. The presence of various mutations detected in virulence-associated mobile elements indicates high genetic similarity of the strains reported here with new highly virulent variants of the cholera agent V. cholerae.
Highlights
We present the draft whole-genome sequence of Vibrio cholerae O1 El Tor strains 76 and M3265/80, isolated in Mariupol, Ukraine, and Moscow, Russia
A comparative analysis of the virulence-associated mobile elements of strain 76 — such as prophages cholera toxin prophage (CTX), TLC, and RS1, Vibrio pathogenicity islands 1 (VPI-1) and 2 (VPI-2), Vibrio seventh pandemic islands I (VSP-I) and II (VSP-II), as well as integrative conjugative elements of the SXT family, which carry the genes involved in multidrug resistance—showed high nucleotide sequence similarity to the same regions of the new hypervirulent El Tor variants isolated in 2010 from a cholera outbreak in Haiti (3, 5)
In contrast to El Tor variants possessing the cholera toxin prophage CTX containing the classic allele of the ctxB gene, ctxB1, strain 76 carried a novel allele, ctxB7
Summary
We present the draft whole-genome sequence of Vibrio cholerae O1 El Tor strains 76 and M3265/80, isolated in Mariupol, Ukraine, and Moscow, Russia. The libraries were prepared using Ion PGM preparation protocols (Thermo Fisher Scientific Inc., USA) and sequenced on the Ion PGM platform, generating 1.0 million (~200-bp) reads (35ϫ genome coverage) and 1.8 million (~400-bp) reads (58ϫ genome coverage) for 76 and M3265/80, respectively. De novo genome assembly was performed using Newbler version 2.6. Draft genomes were annotated using the NCBI Prokaryotic Genome Annotation Pipeline.
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