Abstract
ABSTRACTWe report here the draft genome sequences of two Arthrobacter strains isolated from a holm oak forest affected by wildfire. Both strains were shown to act as plant growth promoters, with AFG20 being a member of the most abundant group found in this soil and AFG7.2 being the strain with the highest indole-3-acetic acid production level.
Highlights
We report here the draft genome sequences of two Arthrobacter strains isolated from a holm oak forest affected by wildfire
A total of 9,287 contigs were constructed for the isolate AFG7.2 and 7,258 contigs for AFG20
Contigs that were bigger than 500 bp, mapped by more than 1,000 reads with concordance in the BLASTn best hit and in the mean coverage of each column (MCC), were retained (Table 1)
Summary
We report here the draft genome sequences of two Arthrobacter strains isolated from a holm oak forest affected by wildfire. Genomic DNA was isolated using the RealPure genomic DNA extraction kit (Durviz, Valencia, Spain), with the following modifications to improve the cellular lysis: keep the centrifuged bacterial culture at Ϫ20°C for 24 h and incubate it twice with lysozyme. Whole-genome sequencing was performed using the Kapa DNA library preparation kit, using 100-bp paired-end sequencing by Illumina. Sequence trimming was carried out in CLC Genomics Workbench 9.0.1.
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