Abstract
Salmonella enterica subsp. enterica serovar Typhimurium WG49 is widely used for enumeration of F-specific RNA (F-RNA) coliphages in water. Escherichia coli WG5 is broadly used for the detection and enumeration of somatic coliphages in water samples. We report here the genome sequences of these bacterial strains used in South Africa under ISO methods 10705-1 and 10705-2.
Highlights
Salmonella enterica subsp. enterica serovar Typhimurium WG49 is widely used for enumeration of F-specific RNA (F-RNA) coliphages in water
Annotation was performed with Rapid Annotation using Subsystems Technology (RAST) [13], giving rise to 4,345 protein coding regions, 31 rRNAs, and 83 tRNAs
No antibiotic resistance genes were identified by ResFinder [16], but 15 were found by the Antibiotic Resistance Genes Database (ARDB) finder [17]
Summary
Salmonella enterica subsp. enterica serovar Typhimurium WG49 is widely used for enumeration of F-specific RNA (F-RNA) coliphages in water. Bacterial DNA was isolated with a NucleoSpin tissue kit (Macherey-Nagel) and subjected to Nextera XT DNA library preparation according to Illumina (USA) instructions. Normalized libraries were run on an Illumina MiSeq sequencer (2 ϫ 300nucleotide [nt] paired-end reads, 1,570,000 reads, 100ϫ estimated coverage). Read quality was evaluated with FastQC (https://www.bioinformatics.babraham.ac.uk/projects/ fastqc/).
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