Abstract
Previously obtained highly immunogenic Env-VLPs ensure overcoming the natural resistance of HIV-1 surface proteins associated with their low level of incorporation and inaccessibility of conserved epitopes to induce neutralizing antibodies. We also adopted this technology to modify Env trimers of ZM53(T/F) strain to produce Env-VLPs by recombinant vaccinia viruses (rVVs). These rVVs expressing Env, Gag-Pol (HIV-1/SIV), as well as the cowpox virus hr gene allowing to avoid the restriction of vaccinia virus replication in CHO cells were used for VLP production. The CHO Lec1 engineered cell line lacking GlcNAc-TI was used for generating VLPs with Env proteins containing a cytoplasmic domain (CT) affecting on surface subunit (SU) conformation. This has created the opportunity to modulate the glycan composition, and refine the conditions for their production, and optimize approaches to overcoming HIV-1 resistance associated with abundant glycosylation.
Published Version
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