Abstract
Plants are rich in bioactive chemical secondary metabolites and have proven insecticidal activity by killing or repelling insects. In this work, we aim to evaluate the direct and delayed effects of ethanolic plant extracts on the vinegar fly (Drosophila melanogaster). The treatment was performed by ingestion on second instar larvae (L2) to evaluate the impact of the ethanolic extract on development for 15 days and subsequently on the feeding behavior of the larvae. The results of this study indicate a slowing down of pupal growth until the adult stage, at the three concentrations (0.25 µg/ml, 0.5 µg/ml, 1.5 µg/ml, 2 µg/ml) used. The results also showed that after three days of treatment, third instar D. melanogster larvae lost the ability to detect the odors of their nutrient environments. Other numbers of larvae (34 %) do not make a choice in the different tests performed. This study indicates that the ethanolic extract of Ramalina farinacea has a neurotoxic property our results confirmed the presence of toxic secondary metabolites which have bioinsecticidal activities in this extract.
Highlights
Plants produce active substances with insecticidal, aseptic or plant and insect growth regulating properties
This study indicates that the ethanolic extract of Ramalina farinacea has a neurotoxic property our results confirmed the presence of toxic secondary metabolites which have bioinsecticidal activities in this extract
We introduce the filter papers in tubes containing the control culture medium mixed with the ethanolic extract of Ramalina farinacea and we leave them for 2 hours so that the filter papers keep the smell of the food
Summary
Plants produce active substances with insecticidal, aseptic or plant and insect growth regulating properties Most often, these active substances are secondary metabolites that originally protect plants from herbivores (Schmutterer, 2002). Biopesticides, which are based on natural plant extracts, are the best alternative means of controlling insects. They are less expensive, effective and without risk for the environment and human health. The most widespread species in the world is considered a dreaded pest both for the inconvenience caused by parasitic diseases that it can inoculate (Jolivet, 1980; Joly, 2006; Habbachi et al, 2013). It is an important vector for various infectious microorganisms for crops (yeasts and phytopathogenic bacteria) (Kloepper et al, 1979; Corby-Harris et al, 2007; Nadarasah and Stavrinides, 2011; Becher et al, 2012)
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