Abstract
Genetic studies through molecular markers proved important to find out the genetic diversity of canola. In this study, 50 lines of canola were used to find the polymorphism using 15 SSR primers and investigated the genetic diversity, PIC values, frequency-based genetic distance, and allelic frequencies. Mean gene diversity, frequency-based genetic distance, and PIC values were 0.8777, 0.233 and 0.8666, respectively for the canola lines. A good range of genetic diversity was found among studied canola lines with value 85.91% polymorphism. Maximum and minimum genetic distances among 50 lines were 1 and 0.26, respectively. Accessions ACC-26068, ACC-24241, ACC-24244, ACC-24233, ACC-24423 and ACC-24224 have maximum genetic distance. Accessions ACC-24879 and ACC-24169 had minimum genetic distance i.e., 0.26. Dendrogram based on genetic distances showed four main clusters that were further dividing into several sub-clusters. The primers utilized in the present study, were valuable to identify different accessions of canola to find the variability present. This variability will be helpful to initiate the breeding program with their molecular genetic basis.
Highlights
Canola has been cultivated for many years, used as a resource of edible oil and have a wide range of usage in industries to manufacture bioproducts
Total 15 Simple sequence repeats (SSR) primers were used to analyze the genetic diversity in 50 canola cultivars
Results of experiments carried out in this study indicated the capability of SSR markers to distinguish between B. napus cultivars
Summary
Canola has been cultivated for many years, used as a resource of edible oil and have a wide range of usage in industries to manufacture bioproducts. Molecular markers have been developed and show a wide range of applications in molecular biology that involve ecology, population genetics, evolution, phylogenetic study, and study genomic traits like hair and skin color in both animal and plants. These various applications can be achieved because of more advances in molecular techniques (Morin et al, 2004; Park et al, 2021). Because of their robust nature, codominant, multi-allelic, hyper-variable, and locusspecific type (Kong et al, 2000; Oraguzie et al, 2007; Murray et al, 2009) The objective of this investigation was to evaluate genetic diversity and polymorphic information contents (PIC) between canola cultivars by using SSR markers
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