Abstract
Natural polysaccharides derived from Hemerocallis citrina Baroni exhibit significant biological activity. To enhance extraction efficiency and antioxidant activity, ultrasonic-assised plasma-activated water (PAW) was utilized to extract polysaccharides from Hemerocallis citrina Baroni. PAW demonstrated enhanced permeability and diffusion capabilities due to a reduced percentage of fully hydrogen-bonded structures, along with optimal pH (3.4) and conductivity (157.20μS/cm). Compared to other methods, PAW significantly improved the extraction efficiency of polysaccharides to 38.24%. Hemerocallis citrina Baroni polysaccharides predominantly consisted of α-pyranose sugars with sugar uronic acids, and ultrasound-assisted PAW did not alter their major functional groups. The extracted Hemerocallis citrina Baroni polysaccharides exhibited a semi-rigid triple-helical structure. Importantly, these polysaccharides displayed strong antioxidant activity, with ABTS+, DPPH, and O2- radical scavenging rates of 66.31%, 65.98%, and 47.73% respectively. This study provides an effective method for extracting natural plant polysaccharides and offers valuable insights into their potential applications in the food industry and beyond.
Published Version
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