Abstract
BackgroundVegetable ‘mandi’ (road-side vegetable market) waste was converted to a suitable fermentation medium for cultivation of oleaginous yeast Rhodosporidium toruloides by steaming under pressure. This cultivation medium derived from waste was found to be a comparatively better source of nutrients than standard culture media because it provided more than one type of usable carbon source(s) to yeast.ResultsHPLC results showed that the extract contained glucose, xylose and glycerol along with other carbon sources, allowing triauxic growth pattern with preferably usage of glucose, xylose and glycerol resulting in enhanced growth, lipid and carotenoid production. Presence of saturated and unsaturated fatty acid methyl esters (FAMEs) (C14-20) in the lipid profile showed that the lipid may be transesterified for biodiesel production.ConclusionUpscaling these experiments to fermenter scale for the production of lipids and biodiesel and other industrially useful products would lead to waste management along with the production of value added commodities. The technique is thus environment friendly and gives good return upon investment.
Highlights
Vegetable ‘mandi’ waste was converted to a suitable fermentation medium for cultivation of oleaginous yeast Rhodosporidium toruloides by steaming under pressure
R. toruloides efficiently utilized more than one carbon source for growth and lipid formation as indicated by its growth curve
Analysis of the growth curve of R. toruloides using waste extract (WE) as culture medium showed that there was a significant increase in cell mass after 6 h of cultivation (Fig. 2b), whereas in case of minimal media (MM), the increase was observed after 22 h
Summary
Vegetable ‘mandi’ (road-side vegetable market) waste was converted to a suitable fermentation medium for cultivation of oleaginous yeast Rhodosporidium toruloides by steaming under pressure This cultivation medium derived from waste was found to be a comparatively better source of nutrients than standard culture media because it provided more than one type of usable carbon source(s) to yeast. European Union (EU) data showed that EU accounts for 53% of the world’s biodiesel production in 2010 [4] It has been projected by the International Energy agency that by 2050, biofuels will be able to meet at least a quarter of world’s fuel demand [5]. Various feedstock can be used to produce biodiesel including oil extracted from plants or animals [6, 7], plant based or animal based oils contest for water and land, otherwise utilized for farming practices. Microbial oils exhibit several advantages over vegetable oils, due to the short doubling times exhibited by microbes and the growth is less impacted by spatial and
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