The use of salicylaldehyde derivatives as a nitrogen source for antibiotic production by Streptomyces hygroscopicus CH-7

Sandra Konstantinovic,Gordana Gojgic-Cvijovic,Jovan Ciric,Vlada Veljkovic,Milica Zlatkovic,Slavica Ilic

doi:10.2298/hemind170124011k

Abstract

In the present work, four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. The obtained compounds were identified by elemental microanalysis, as well as FTIR, UV/Vis and 1H-NMR spectroscopic methods. Yields of 93?98% of the compounds in crude glycerol were achieved within 10?25 min. The derivatives of salicylaldehyde and crude glycerol were used as a nitrogen and carbon source, respectively, in the medium for antibiotic (hexaene H-85 and azalomycine B) production by Streptomyces hygroscopicus CH-7. The highest concentrations of hexaene H-85 and azalomycine B were achieved in the medium containing salicylaldehyde-thiosemicarbazone (198 and 69 ?g/cm3, respectively). Derivatives of salicylaldehyde also impacted the strain morphology. In the media with salicylaldehyde- phenylhydrazone and salicylaldehyde-thiosemicarbazone, S. hygroscopicus CH-7 grew like large dispersive pellets with long twisted filaments that produced the highest yield of the antibiotics.

Highlights

In the present work, four derivatives of salicylaldehyde were synthesized using both conventional (95% ethanol) and green solvents
Several derivatives of salicylaldehyde were synthesized in crude glycerol that originated from the alkali-catalyzed transesterification of edible sunflower oil as well as in ethanol (95 vol.%) as a conventional solvent The main goal was to increase the product yield and to shorten the reaction time, as well as to evaluate the obtained derivatives as a nitrogen source in the nutrition medium in antibiotic production by S. hygroscopicus CH-7
Dard solvent 95% ethanol, and in crude glycerol, a green solvent obtained as a byproduct of the biodiesel production

Summary

Introduction

Four derivatives of salicylaldehyde (salicylaldehyde-hydrazone, phenylhydrazone, semicarbazone and thiosemicarbazone) were synthesized using both conventional (95% ethanol) and green (crude glycerol from biodiesel production) solvents. Several derivatives of salicylaldehyde (salicylaldehyde-hydrazone, salicylaldehyde-phenylhydrazone, salicylaldehyde-semicarbazone and salicylaldehyde-thiosemicarbazone) were synthesized in crude glycerol that originated from the alkali-catalyzed transesterification of edible sunflower oil as well as in ethanol (95 vol.%) as a conventional solvent The main goal was to increase the product yield and to shorten the reaction time, as well as to evaluate the obtained derivatives as a nitrogen source in the nutrition medium in antibiotic production by S. hygroscopicus CH-7. The reaction occurred without the use of catalyst at 80 °C, and compared to the conventional solvent, the reaction times were shorter and the yields of salicyladehyde derivatives were higher (∆ = 2– 8%), Table 1.

Results

Conclusion