Abstract
The aim of the presented work was to investigate the potential of aqueous extract of cultivated strawberry (Fragaria x ananassa Duch.) leaves for stabilization of silver nanoparticles (AgNPs-E) synthesized at room (RT) and boiling temperature (BT). The synthesis and stability of AgNPs-E were monitored by UV-Vis spectroscopy confirming high stability of the AgNPs-E in the dark at room temperature. The Fourier-transform infrared spectra suggest that molecules containing oxygen and nitrogen functional groups (NH, (NH)C=O, CNO, C-O-C and OH) participate in the reduction and stabilization of formed nanoparticles. As determined by the DPPH test, AgNPs-E synthesized at RT exerted higher antioxidant activity as compared to AgNPs-E synthesized at BT (EC50 values of 0.025 and 0.039 mg cm-3, respectively). Also, the AgNPs-E synthesized at RT exerted higher antibacterial activity against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus subtilis and Bacillus luteus. Examination of the AgNPs-E on HeLa and MDCK cell lines showed concentration-dependent and cell line specific effects on the cell viability as evaluated by the MTT test. The obtained results indicate that synthesized AgNPs-E can be used as a base material in production of pharmaceutical preparations for potential skin applications.
Highlights
Biosynthesis of nanoparticles is gaining an increasing attention as a multidisciplinary field due to the growing need for development of environmentally friendly technologies [1]
Increasing of the band intensity during synthesis (Fig. 1) is the evidence of the AgNP formation; the increase is proportional to the number of nanoparticles formed, while the band position is directly related to the particles size [19]
The prediction of nanoparticle size is supported by already published results where AgNPs were synthesized and stabilized by an aqueous extract of Fumaria officinalis L. by the same procedure, where the nanoparticle size was determined as 21±1 nm and 18±1 nm by XRD and SEM methods [4]
Summary
Biosynthesis of nanoparticles is gaining an increasing attention as a multidisciplinary field due to the growing need for development of environmentally friendly technologies [1]. Synthesis of AgNPs depends on the type of solvent used as well as on reducing and stabilizing agents, which determine whether it is a green or chemical/physical synthesis process. Water is the most commonly used solvent in green AgNP synthesis, with additional advantages of lower energy consumption, simple performance, lower costs, utilization of non-toxic chemicals as reducing and stabilizing agents, which allow biocompatibility and in vivo applications [4,5,6,7,8]. AgNPs are widely applied because of their strong antibacterial activity against both Gram-positive and Gram-negative bacteria They are used in commercial products, such as products for personal hygiene, detergents, cosmetics, sun lotions as well as in medical, textile and food products [9]
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