Abstract
1. The puromycin-mediated release of labelled-nascent proteins from bound ribosomes was examined in rat liver microsomes labelled in vitro with [ 14C]leucine. Subfractions were prepared from labelled control and puromycin-treated microsomes either by treatment with 0.25 % sodium deoxycholate or by sonication. 2. When the microsomes were fractionated by sodium deoxycholate, the distribution of the acid-insoluble radioactivity among the microsomal subfractions indicated that the puromycin-mediated release could be directed either toward the microsomal membrane or toward the intravesicular space. An unequivocal interpretation is not possible because of subfraction contamination and protein binding phenomena. 3. However, the distribution of radioactivity following gel filtration of deoxycholate-solubilized labelled microsomal membranes, or dispersion of the labelled microsomes by sonication, suggest that the direction of the puromycin-mediated release is toward the microsomal membrane rather than toward the intravesicular lumen.
Published Version
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