Abstract
The covalent structure of the rat 40 S ribosomal subunit protein S12 was determined from the sequence of amino acids in tryptic, chymotryptic, thermolytic, and cyanogen bromide peptides and inferred from the sequence of nucleotides in a recombinant cDNA. Rat ribosomal protein S12 contains 129 amino acids and has a molecular weight of 14,120. The amino acid sequences of a number of ribosomal proteins appear to be related to rat S12. These include spinach chloroplast L7, Escherichia coli S5, Nicotiana tabacum chloroplast S18, and Bacillus stearothermophilus S12, and perhaps others. In addition, there are two sequences, 26 and 18 amino acids in length, in rat S12 that may be related to segments of the same number of residues in ribosomal proteins from a number of species. These, and other results, reinforce the suggestion that ribosomal proteins form an extended family.
Highlights
T H E RELATIONSHIP OF RAT S12 TO OTHER RIBOSOMAL PROTEINS AND A CORRELATION OF THE AMINO ACID SEQUENCES OF RAT AND YEAST RIBOSOMAL PROTEINS*
40 S ribosomal confirmed, from the sequence of nucleotides in a recombinant subunit proteinS12 was determined from the sequencecDNA
We present here the amino acid sequence of the 40 S ribosomal subunitprotein S12
Summary
GAT GGe eTA GeA eGT GGe ATA esc GAA GeT Gee AU Gee TlA GAe UG ccc eu Gee eAT CTe TGe GTG eTT GeA Tee ue TGT GAT GA(;. An additional identity is between rat L30 [13] and S. cerevisae L32.5 In an alignment of the amino acid sequences, there are 61 identities in 105 possible matches between residues; that is 58% identity for an alignment score of 28.5 8.D. The number of related residues is increased to 72 (67%) if conservative substitutions are taken into account. There are 14 identities out of 26 possible matches between residues (reduced from 29 since 3 residues in the sequence were not identified) in the alignment or 54% (Table III); the related amino acids are increased by three if a conservative substitution (arginine/lysine) is taken into account and a glutamic acid/glutamine and an aspartic acid/ asparagine ambiguity are scored; that would give 17 out of 26 or 65%. Of the 16 rat ribosomal proteins whose amino acid sequence has been determined, 10 are related to a yeast protein, either from S. cerevisae or S. pombe or both (Table IV).
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