Abstract

The amino acid sequence of the rat 60 S ribosomal subunit protein L23a was deduced from the sequence of nucleotides in a recombinant cDNA. Ribosomal protein L23a has 156 amino acids and a molecular weight of 17,684. Hybridization of the L23a cDNA to digests of nuclear DNA suggests that there are 18-20 copies of the L23a gene. The mRNA for the protein is about 600 nucleotides in length. Rat L23a is related to the yeast Saccharomyces cerevisiae L25, to the archaebacterial Methanococcus vannielii L23, to eubacterial Escherichia coli L23, and to other members of the L23 family of ribosomal proteins. A novel application of a routine homology search procedure was employed to identify a nucleotide sequence that could be used to design an oligodeoxynucleotide probe to screen a library for a cDNA that encodes rat L23a; this same procedure uncovered a number of previously unidentified genes for yeast ribosomal proteins in the GenBank DNA data base. In a correlation of rat and yeast ribosomal proteins 48 pairs are shown to be related.

Highlights

  • The amino acid sequence of the rat 60 S ribosomal Escherichia coli L23, ribosomalproteins that bind to a specific subunit protein L23a was deduced from the sequence site in domain I11of26 S and of23 S rRNAs (2, 3); these of nucleotides in a recombinant cDNA

  • Ribosomal protein L23a has a similar amino acid sequence that is likely to be important for binding to the homologous site in rat 28 S rRNA

  • Routine homology search procedure was employed to identify a nucleotide sequence that could be used to design an oligodeoxynucleotide probe to screen a li- The recombinant DNA procedures and themethods used to deterbrary for a cDNA that encodes rat L23a; this same procedure uncovered a number of previously unidentified genes for yeast ribosomal proteins in the Gen-Bank DNA database

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Summary

The UseofHomology Search to Identify Yeast Genes That

Encode Ribosomal Proteins-The yeast homologs of 48 rat ribosomal proteins are known (Table I); there. Ribosomal proteins by searching the GenBank DNA data base. The search was predicated on the possibility that the sequences of nucleotides in genes for some yeast ribosomal proteins had been determined but not recognized

Rat ribosoYmreaiablsotsomal proteins”
CT A
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