A leucine zipper-like motif and a basic region-leucine zipper-like element in rat ribosomal protein L13a. Identification of the tum- transplantation antigen P198.

Y.L Chan,J Olvera,A Glück,I.G Wool

doi:10.1016/s0021-9258(17)37502-6

Abstract

The amino acid sequence of the rat 60 S ribosomal subunit protein L13a was deduced from the sequence of nucleotides in two recombinant cDNAs. Mature ribosomal protein L13a has 202 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and a M(r) of 23,330. Hybridization of the L13a cDNA to digests of nuclear DNA suggests that there are 9-11 copies of the L13a gene. The mRNA for the protein is approximately 800 nucleotides in length. Rat L13a is related to the Saccharomyces cerevisiae ribosomal proteins that have been provisionally designated rp22 and rp23 and to the eubacterial and archaebacterial family of L13 ribosomal proteins. The mouse tum- transplantation antigen P198 is a mutant of the mouse homolog of rat ribosomal protein L13a. Rat ribosomal protein L7 has, at its NH2 terminus, five tandem repeats of a similar sequence of 12 amino acids (Lin, A., Chan, Y. L., McNally, J., Peleg, D., Meyuhas, O., and Wool, I. G. (1987) J. Biol. Chem. 262, 12665-12671); L13a has, in its carboxyl-terminal region, amino acid sequences with significant identity to L7 repeats 1, 3, and 5. L13a also has a number of short amino acid sequences that are repeated, a leucine zipper-like motif at its NH2 terminus, and a potential basic region-leucine zipper element in its carboxyl-terminal region.

Highlights

The amino acid sequence of the rat 60 S ribosomal structure [4]very much like that found in proteins thatbind to subunit proteinL13a was deduced from thseequence of DNA and that regulate transcription(51, and the acidic ribonucleotidesintworecombinant cDNAs
We report here the aminoacid secDNA to digests of nuclear DNA suggests that there are quence of rat ribosomal protein L13a and the identi~catoiof an
The rat ribosomal proteins S27 and S29 have erating rat liver poly(A)+m ~ A ( 1 0, l lw)as screened for clones single zinc finger domains of the C2-C2type [2]; this that hybridized to a probe (synthesized in thepolymerase chain element is found in proteinsfrom the ribosomes of eubacteria, reaction [15])for a rat ribosomal protein L13a cDNA

Summary

RESULTS

ANDDISCUSSION of these datafor mammalian (rat)ribosomes [1].The sequence The Sequences of Nucleotides in Recombinant cDNAs Encodof amino acids in rat ribosomal protein L13a is a cont~butionto ing RaRt ibosomal ProteinLI3u-A random selectionof 48,000 this project. The sequences of nucleotides were determined in both strands of the cDNA inserts intwo of the. 127nucleotides of a n open reading framea, 3"noncoding region of 25 bases, and a long poly(A) tail (Fig. 1). FIG.1."he sequence ofnucleotides in thCeDNAinsertsin plasmids pL13a-21 and pL13a-11 anthde amino acid sequence encoded in the open reading frameT. Nucleotides of an open reading frame(Fig. 1).The overlapping sequences in pL13a-11and pL13a-21 are identical; they are likely to be derived from the same gene. Given below is the amino acid composition(in numbers of residues) determined either (A) from a hydrolysate of purified L13a [19] or inferred (B) from the sequence of nucleotides in pL13a-11,21. The cDNAinsert in pL13a-11,21has a B'-noncoding sequence of 15 nucleotides, a single open reading frame of 612, a 3'noncoding region of 25, and a long poly(A) tail. The open read ing frame starts at anATG codon at a position that we designate +1and endswith a termination codon (TGA) at position

His Ile

VLDG LLGR

Lz u

Basic domain