The primary structure of rat ribosomal protein S5. A ribosomal protein present in the rat genome in a single copy.

Y Kuwano,J Olvera,I.G Wool

doi:10.1016/s0021-9258(19)74040-x

Abstract

The amino acid sequence of the rat 40 S ribosomal subunit protein S5 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed by the determination, directly from the protein, of 17 residues near the NH2 terminus. S5 has 204 amino acids; the molecular weight is 22,863. The protein designated S5a has the same amino acid sequence as S5 except that it lacks the NH2-terminal 5 residues. It is not known whether the conversion of a portion of S5 to S5a is physiological or fortuitous. The mRNA for S5 has about 820 nucleotides. Hybridization of the S5 cDNA to digests of nuclear DNA indicates that the rat genome has only a single copy of the gene; this is in distinction to the mouse and human genomes which have three to six copies of the S5 gene. Rat ribosomal protein S5 is related to the eubacteria, the arachaebacteria, and the chloroplast family of S7 ribosomal proteins. There is a peptide of 16 residues at the carboxyl terminus of S5 that is highly conserved in 18 species spanning the three kingdoms and chloroplasts.

Highlights

The amino acid sequence of the rat 40 S ribosomal related eukaryotic S5 are likely to be near the decoding site subunit protein S6 was deduced from the sequence of in the small subunit [2]
The S5gene is unique amongst the nucleotides in a recombinant cDNA and confirmed by mammalian ribosomal protein genes that have been analyzed the determination, directly fromtheprotein, of 17 in being present in the ratgenome in a single copy. residues nearthe NH2 terminus
A probe for the cDNA encoding rat ribosomal protein S5a, whose design was based on the sequence ETPDIKLFGK, was contained in amixture of 54 different oligodeoxynucleotides,each 29 bases long, that was synthegenome has only a single copy of the gene; this is in sized on a solid support by the methoxyphosphoramiditemethod distinction to the mouse and human genomes which using an Applied Biosystems, model 380B, DNA synthesizer [6]

Summary

Arginine Aspartic acid and asparagine

9 +1713 has been reported [16] to favor NHz-terminalprocessing. The amino acid composition of S5 deduced from the sequence of nucleotides in pS5-1 is very closeto thadt etermined directly from the protein [15]; the amino acid compositions of S5 and S5a, the latter determined from.

NDb ND

Valine Residues

The Identification of a Highly Conserued Sequence of Amino

AVKKKEETHRMAEANR IR