Abstract
Aerobic glycolysis is commonly used as the main energy source for cancer cell growth. Adenylate kinase 2 (AK2) plays an important role in maintenance of ATP production in the mitochondria. Neoandrographolide in Andrographis paniculata potentially inhibit AK2 activity in silico. This study aimed to investigate the inhibitory effect of A. paniculata extract on proliferation of breast cancer cell line. This experimental laboratory used MCF-7 cell line and A. paniculata plants, which were extracted using the maceration method with 70% ethanol. Neoandrographolide concentration in A. paniculata extract was determined using HPLC. A total 1x104 MCF-7 cells were incubated for 24 hours with or without 0.4% (v/v) dimethyl sulfoxide as solvent (SC) or negative control (NC) group. The same amount of MCF-7 cells was treated with A. paniculata extract with 3 different dosages: 222 ppm (AE1), 111 ppm (AE2) and 55.5 ppm (AE3). The inhibition of cell proliferation used the 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide (MTT) assay. Data were analyzed using ANOVA and LSD tests with p value <0.05.Ethanol extract of A. paniculata contained 4.43 µM neoandrographolide. The inhibition of MCF-7 cell proliferation was found in AE1 (47.98%) and AE2 (30.50%) groups and was significantly different from the NC group. While the percentage of MCF-7 cell inhibition in the AE1 group differed from NC (p = 0.002) and SC (p = 0.013) groups. Ethanol extract of A. paniculata can inhibit MCF-7 cell proliferation. Further Study is needed to investigate the role of neoandrographolide on inhibition of AK2 activity in MCF-7 cells.
Published Version
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