The expression and significance of kang ai 1 protein in colorectal adenoma and colorectal carcinoma

Abstract

Objective To observe the expression and clinical significance of kang ai 1 (Kai1) in the process from the progression from colorectal polyps to cancer, invasion and metastasis. Methods Immunohistochemistry was used to examine the expression of Kai1 in the colorectal tissues, colorectal adenoma tissues and colorectal carcinoma tissues. Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect Kai1 mRNA expression. Results The expression of Kai1 protein was positive in 18 cases of colorectal carcinoma (45.00%), significantly lower than that in colorectal adenoma (91.25%) and colorectal tissues (100.00%), respectively, with significant differences among them (P=0.031). FQ-PCR showed that the expression of Kai1 mRNA in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa was incresed by 2.038±0.933, 9.983±4.337 and 12.260±2.182 respectively (F=11.960, P=0.015). The expression of Kai1 protein and mRNA had a significant correlation with the lymph node metastasis of patients with colorectal carcinoma (P=0.011). Conclusion The abnormal expression of Kai1 protein and mRNA is related with the carcinogenesis and the development of colorectal carcinoma. Key words: Kang ai 1; Colorectal carcinoma; Gene expression