Abstract
Objective To investigate the expression and significance of Kiss1 protein in colon adenoma and colon carcinoma. Methods Immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) were used to detect the location and expression of Kiss1 in the 20 normal colon mucosae, 30 colon carcinomae, 80 colon adenomae. Results The positive expression rate of Kiss1 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 93.3%, 58.8% and 5.0% respectively, with significant differences among them (P=0.005). The positive expression rate of Kiss1 protein in tubular adenoma, mixed pattern of adenoma and villous adenoma was 80.0%, 66.7% and 35.0% respectively, with significant differences among them (P=0.005). FQ-PCR showed that the Kiss1 mRNA expression rate was 28.85±8.34, 14.61±1.65, 7.58±1.31, 2.26±1.56 and 0.50±0.40 in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma, respectively. Conclusion Kiss1 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma. Detecting the expression of Kiss1 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma. Key words: Colorectal adenoma; Colorectal carcinoma; Kiss1
Published Version
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