The expression and significance of metastasis suppressor 1 in colon adenoma and colon carcinoma

Abstract

Objective To investigate the expression and significance of metastasis suppressor 1 (MTSS1) protein in colon adenoma and colon carcinoma. Methods Immunohistochemistry and fluorescence quantitative-polymerase chain reaction (PCR) were used to detect the location and expression of MTSS1 in the 20 normal colon mucosa, 30 colon carcinoma, 80 colon adenoma samples. Results The positive expression rate of MTSS1 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues were 100.0%, 63.8%, 6.7%, respectively, with significant differences among them (P=0.005). The positive expression rate of MTSS1 protein in tubular adenoma, mixed pattern of adenoma and villous adenoma were 82.5%, 56.7%, 35.0%, respectively, with significant differences among them(P=0.005). Fluorescence quantitative PCR showed that the MTSS1 mRNA expression are 36.58±9.06, 16.34±1.34, 10.47±3.20, 3.40±1.28, 0.80±0.40. Conclusion MTSS1 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma. Detecting the expression of MTSS1 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma. Key words: Colorectal adenoma; Colorectal carcinoma; Immunohistochemistry; Fluorescence quantitative-polymerase chain reaction; Metastasis suppressor 1