The expression and significance of calcium channel activated protein 43 in colorectal adenoma and colorectal carcinoma

Abstract

Objective To explore the expression of calcium channel activated protein 43 (Cap43) in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma, and to analyze the relationships among different histological types of colorectal adenomas and colorectal carcinoma. Methods Immunohistochemistry was used to exzamine the location and expression of Cap43 in the colorectal tissues, colorectal carcinoma tissues and colorectal carcinoma tissues. Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect Cap43 mRNA expression. Results The positive expression rate of Cap43 in normal colorectal mucosa, colorectal colorectal adenoma and and carcinoma tissues was 31.7%, 63.8% and 91.7% respectively, with significant differences among them (P=0.000). The positive expression rate of Cap43 in tubular adenoma, mixed pattern of adenoma, and villous adenoma was 52.5%, 60.0% and 90.0% respectively, also with significant differences among them (P=0.016). The Cap43 mRNA expression of the colorectal carcinoma, villous adenoma, the mixed pattern of adenoma, tubular adenoma, and normal colorectal mucosa was 19.552±6.338, 13.018±2.417, 7.323±2.131, 3.660±1.309, and 1.119±0.871 respectively (F=25.943, P=0.028). Conclusion Cap43 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma. Detecting the expression of Cap43 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma. Cap43 may be the molecular biological indicator for predicting canceration of colorectal adenoma. Key words: Colorectal adenoma; Colorectal carcinoma; Calcium channel activated protein 43