Abstract
A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min-1. Hydrodynamic voltammetric studies were undertaken to optimize the operating potentials required for electrochemical detection. It was found that an applied potential of +1.2 V was optimum for the determination of uric acid. The proposed method was evaluated by carrying out replicate uric acid determinations on spiked and unspiked human saliva samples (%CV = 8.8 %). The data suggest that the method holds promise for clinical applications.
Highlights
The aim of the current study was to investigate the possibility of determining uric acid (2,6,8-trihydroxypurine) (I) in human saliva using high performance liquid chromatography and electrochemical detection (LC-ECD)
Investigations showed that an organic modifier concentration of 2 % methanol was insufficient to stop collapse of the stationary phase and so was increased to 5 % and a mobile phase of 5 % methanol, 95 % 50 mM trichloroacetic acid (TCA) adjusted to pH 2.7 was found to optimal, and was used in further studies
An assay involving LC-ECD has been successfully developed for the determination of endogenous levels of uric acid in human saliva
Summary
The aim of the current study was to investigate the possibility of determining uric acid (2,6,8-trihydroxypurine) (I) in human saliva using high performance liquid chromatography and electrochemical detection (LC-ECD). The study of uric acid is important as it represents one of the most important antioxidants in humans contributing to more than 70 % of the total antioxidants present in human saliva[1]. Humans and other higher apes exhibit relatively high levels of uric acid and in blood it is near its solubility point. Uric acid has been shown to be associated with conditions such as, type 2 diabetes[5], congestive heart failure, hyperuricemia obesity, hypertension[6], depression[7], cognitive ability[8], Lesch-Nyhan syndrome[9] and kidney stone formation[10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
