Studies on antibacterial efficacy of ZnO nanoparticles using Tanner's Cassia flower extract from green synthesis

Abstract

Sodium Hydroxide is used in the green synthesis approach (chemical precipitation method) to produce zinc oxide nanoparticles from zinc nitrate and extracts of Tanner's Cassia.The produced nanoparticles are tested using techniques like powder X-ray diffraction (XRD), Diffuse Reflectance Spectroscopy (DRS), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Analysis (EDAX) and antimicrobial activity. The X-ray diffraction Scherer's formula is used to determine the size of the ZnO particles.The measured size of the particle is 20.82 nm. In the XRD graph, ZnO nanoparticles are visible as crystalline peaks, whereas the rest is amorphous. The functional groups like ZnO, CC and CN bonds are confirmed by the FTIR data. SEM is used to examine the microstructure and identify aggregation. From EDAX, elemental composition of the sample is found to be having Zinc 70.36% and Oxygen 29.64%. The disk diffusion test is performed on solid agar plates and is found that it is effective against E. coli bacteria as an antibacterial substance.