Abstract
Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. We obtained the complete genome of a CVA isolate (CVA-TA) using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The newly identified 5′-untranslated region (5′-UTR) from CVA-TA may form additional hairpin and loop structures to stabilize the CVA genome.
Highlights
Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas
The genomes of capilloviruses consist of two open reading frames (ORFs)
The complete genome sequence of CVA-TA was cloned by overlapping reverse transcription-PCR (RT-PCR). 5= and 3= terminal sequences were cloned by rapid amplification of cDNA ends (RACE)
Summary
Cherry virus A (CVA) (Capillovirus, Betaflexiviridae) is widely present in cherry-growing areas. ORF1 encodes a 226-kDa fusion protein, which includes the RNAdependent RNA polymerase (RdRp) and the coat protein (CP), and ORF2, located within the ORF1 but with a different reading frame, encodes the putative movement protein (MP) (1). We obtained a Chinese CVA isolate and named it CVA-TA. The complete genome of CVA-TA was determined using small RNA deep sequencing, followed by overlapping reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends (RACE).
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