Abstract
Objective: The ozone molecule, composed of three oxygen atoms, possesses high oxidizing power and is widely utilized for various applications, including therapeutic treatments involving the administration of medicinal ozone to combat various diseases. The standardization of ozonized oil is essential to ensure patient safety. The objective of this study was to develop protocols for the standardization and characterization of ozonized oil using analytical techniques such as gas chromatography-mass spectrometry (GC-MS) and to evaluate its biological activity through microbiological and cytotoxic assays. Methods: Standardization and characterization were carried using gas chromatography coupled to mass spectrometry (CG-MS), and microbiological tests including agar diffusion, time-kill and MIC and cytotoxic tests were employed in bacterial cells. Results: The obtained results indicated that after 480 minutes of ozonization in 100 mL of sunflower oil (OG), a significant antimicrobial potential was observed, leading to the formation of ozonides. Antibacterial and antifungal activity assays, conducted using the ‘time kill’ method, agar diffusion, and Minimum Inhibitory Concentration (MIC) tests, demonstrated activity against Staphylococcus aureus, Escherichia coli, Salmonella choleraesuis, Pseudomonas aeruginosa, Candida albicans, Aspergillus brasiliensis, and Malassezia furfur. Conclusion: Results demonstrate the therapeutic potential of the application of ozonized oil in the treatment of topic infectious diseases. In summary, the standardization of the ozonization process of sunflower oil showed to be promising, providing a product exerting antimicrobial activity and therapeutic potential, opening perspectives for its application in various areas of medicine.
Published Version
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