Spectrophotometric determination of sulphacetamide sodium via diazotization and coupling reaction

Abstract

A simple spectrophotometric method has been suggested for the assay of sulphacetamide sodium(SAAMS) through diazotization and coupling. The method include the reaction of SAAMS with sodium nitrite in acidic medium of hydrochloric acid to produce the corresponding diazonium salt-SAAMS(D-SAAMS), which is coupled with 2,4- dihydroxybenzophenone(2,4-DHBP) in presence of sodium hydroxide to produce orange azo dye, water-soluble and stable which shows maximum absorbance at 497.5 nm. . The absorbance was found to increase linearly with the increasing amounts of SAAMS in the solution from 10 to 250 μg in 10 ml (1-25 μg.ml1) . Two important factors were calculated to show the sensitivity of the method: molar absorptivity and Sandell s sensitivity index, have the values 2.27 × 104l.mol−1.cm−1 and of 0.011198 μg.cm−2respectively. Both values demonstrated good sensitivity to the proposed method . SAAMS has been estimated successfully in eyes drops.