DETERMINATION OF PHENYLEPHRINE HYDROCHLORIDE IN PHARMACEUTICAL PREPARATIONS USING SPECTROPHOTOMETRIC METHOD

Wasan A Al-Uzri Wasan A Al-Uzri

doi:10.22159/ajpcr.2019.v12i5.32339

Wasan A Al-Uzri Wasan A Al-Uzri

Open Access

https://doi.org/10.22159/ajpcr.2019.v12i5.32339

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Abstract

Objective: A simple and sensitive spectrophotometric method has been presented for the determination of phenylephrine hydrochloride by coupling reaction with diazotized sulfacetamide sodium. Methods: The method is based on the diazotization reaction of sulfacetamide sodium with sodium nitrite in the presence of hydrochloric acid to form diazonium salt, which is coupled with the drug in alkaline medium to form azo dye, showing absorption maxima at 425 nm. Results: Calibration plot was linear over the concentration range of 2–24 μg/mL and detection limit of 0.278 μg/mL with a correlation coefficient of 0.9929. All different chemical and physical experimental parameters affecting on the development and stability of the colored product were carefully studied. Conclusions: The proposed method was successfully applied to the determination of phenylephrine in nasal drops with good precision and sensitivity.

Highlights

Phenylephrine hydrochloride (PHP), [(R)-1-(3-hydroxyphenyl).2(methylamino) ethanol hydrochloride], is a white crystalline powder, freely soluble in water, melts at 143°C [1,2] and its chemical structure is: It belongs to a group of drugs named sympathomimetics [3]
Determination of absorption maximum An aqueous solution of PHP is reacted with diazotized sulfacetamide sodium in alkaline medium giving yellow dye which became stable after 15 min and has a maximum absorption at 425 nm
Effect of the volume of hydrochloric acid (HCl) (0.5 M) The effect of different volumes (0.3–3 mL) of HCl was examined on the maximum absorbance of the formed product

Summary

Introduction

Phenylephrine hydrochloride (PHP), [(R)-1-(3-hydroxyphenyl).2(methylamino) ethanol hydrochloride], is a white crystalline powder, freely soluble in water, melts at 143°C [1,2] and its chemical structure is: It belongs to a group of drugs named sympathomimetics [3]. It stimulates alpha receptors in certain areas of the body. Various methods reported in literature for analysis of phenylephrine hydrochloride. The most popular and simple method for rapid and trace analysis of drugs is spectrophotometry [19,20,21,22,23,24,25,26]

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