Abstract
A simple, rapid, accurate and sensitive spectrophotometric method has been developed for the quantitative determination of sulfadiazine (SDz) in both pure and its dosage forms. The method is based on diazotization of primary amine group of sulfadiazine with sodium nitrite and hydrochloric acid followed by coupling with γ-resorsolic acid (2,6dihydroxybenzoic acid) in alkaline medium of sodium hydroxide to form a yellow coloured azo dye shows a maximum absorption at 458 nm against reagent blank solution. Beer's law is obeyed over the concentration range of 10-300 µg of SDz / 25 ml (0.4-12 ppm) with a determination coefficient (R 2 =0.9998 ) and molar absorptivity 4.38×10 4 l.mol -1 .cm -1 and a relative error in the range of 0.1- 0.64% and a relative standard deviation from ± 0.27 to ± 1.21 % depending on the concentration level of SDz. The method is suitable for the determination of sulfadiazine in the presence of other ingredients that are usually present in dosage forms. The effect of organic solvents on the spectrophotometric properties of the azo dye and the composition of the resulting product have also been worked out and it is found to be 1:2 γ-resorsolic acid: sulfadiazine. The method has been successfully applied to the determination of sulfadiazine in its pharmaceutical preparations ( tablet, and burn cream ).
Highlights
Sulfadiazine (SDZ), 4-amino-N-pyrimidin-2-yl-benzenesulfonamide, is a sulfonamide group of antibiotic drug which has been used in veterinary and human therapy over 60 years (Pecorelli et al, 2004), it acts by inhibiting the production of folic acid inside the bacterial cell
Among them are reversed phase high performance liquid chromatography coupled with on-line atmospheric pressure chemical ionization mass spectrometry (HPLC,APCI-MS) (Combs et al.,1999), cloud point extraction /flow injection-flame atomic absorption (CPE/FI-FAAS) spectrometry (Dadfarnia et al, 2011), capillary zone electrophoresis (Berzas et al, 2001), inductively coupled plasma-atomic emission spectroscopy (ICP-AES) (Qi-Oi et al, 2010), liquid chromatography (Valentina et al, 2009), UV–spectrophotometry (Kotha-cota et al, 2011), immunochromatographic assay (Wang et al, 2007), flow injection chemiluminescence (Liu et al, 2007), ionselective electrode (Ayman et al, 2009)
We succeeded in developing a new coupling agent for a sensitive and selective spectrophotometric determination of the sulfonamide based on the coupling of diazotized SDZ with γ-resorsolic acid to form a yellow colored product in alkaline medium that has been proved successfully for the determination of SDZ in both pure form and its pharmaceutical preparations
Summary
Sulfadiazine (SDZ), 4-amino-N-pyrimidin-2-yl-benzenesulfonamide, is a sulfonamide group of antibiotic drug which has been used in veterinary and human therapy over 60 years (Pecorelli et al, 2004), it acts by inhibiting the production of folic acid inside the bacterial cell. Many UV-Visible spectrophotometric methods for the determination of SDz have been developed. We succeeded in developing a new coupling agent for a sensitive and selective spectrophotometric determination of the sulfonamide based on the coupling of diazotized SDZ with γ-resorsolic acid to form a yellow colored product in alkaline medium that has been proved successfully for the determination of SDZ in both pure form and its pharmaceutical preparations
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
