Abstract
A simple, precise, rapid, selective, and economic reversed phase high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of Ambroxol Hydrochloride and Doxofylline. The HPTLC method was performed on precoated silica gel G60 F254 plates with Diethyl ether:n-butanol:Ammonia (9:0.9:0.1 v/v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their absorption at approximately 254 nm for AMB and DOX. The drugs were satisfactorily resolved with Rf 0.29 ± 0.02 for and Rf 0.56 ± 0.02 for DOX. The calibration plot was linear between 100- 600 ng /band for AMB and 200-1200 ng/band based for DOX. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined dosage form.
Highlights
Ambroxol hydrochoride (AMB; (Trans-4-(2-amino-3,5dibromobenzylamino) cyclohexanol hydrochloride); (Figure 1a) is a widely secretolytic agent used in the treatment of respiratory diseases associated with viscid or excessive mucus [1]
Procedure for analysis of marketed preparation. 2 microlitre of the sample solutions (100 μg/ml Ambroxol Hydrochloride and 200 μg/ml Doxofylline) was applied on the TLC plate followed by development and scanning
The sensitivity of high-performance thin layer chromatography (HPTLC) method that uses UV detection depends upon proper selection of detection wavelength
Summary
Ambroxol hydrochoride (AMB; (Trans-4-(2-amino-3,5dibromobenzylamino) cyclohexanol hydrochloride); (Figure 1a) is a widely secretolytic agent used in the treatment of respiratory diseases associated with viscid or excessive mucus [1]. Sample solution and mobile phase were prepared in similar way as described earlier. 2 microlitre of the sample solutions (100 μg/ml Ambroxol Hydrochloride and 200 μg/ml Doxofylline) was applied on the TLC plate followed by development and scanning. Preparation of standard solution: A 25 mg of standard AMB and 50 mg of DOX were accurately weighed and transferred to two separate 25 ml volumetric flasks and dissolved in few ml methanol. The flask was shaken and volume was made up to the mark with methanol to yield a solution containing 1000 μg/ml of AMB and 2000 μg/ml DOX. J Chromat Separation Techniq 4: 168. doi:10.4172/2157-7064.1000168
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