Abstract
A high performance liquid chromatographic method for estimation of Transitmycin (Tr) in human plasma was developed. The analyte was extracted using solid phase cartridges and the analysis of the eluent was carried out using Atlantis T3 column (150 cm × 4.6 mm ID) and photo diode array detector set at wavelength of 214 nm. The assay was specific for Tr and linear from 0.5 µg/mL to 20.0 µg/mL. The relative standard deviations for intra- and inter-day assays were less than 10%. The method yielded a recovery for Tr that ranged from 94% to 107% and could be used in toxicology and pharmacokinetic studies.
Highlights
Tuberculosis (TB) is the greatest infectious killer in India as well as in other developing countries and its high incidence worldwide is an important global health concern
We aimed to develop a HPLC-based bio-analytical method for the estimation of Tr in human plasma
Blank plasma sample did not give any peak at the retention time of Tr (Figure 2a)
Summary
Tuberculosis (TB) is the greatest infectious killer in India as well as in other developing countries and its high incidence worldwide is an important global health concern. Several drugs are available for treatment of TB, resistance to existing anti-TB drugs necessitates the development of newer drug molecules and testing them in TB treatment. Transitmycin (Tr) is a novel antibiotic isolated from the producer strain Streptomyces sp. The physico-chemical properties of this compound have been established [1]. This molecule was found to be active against Mycobacterium tuberculosis, Bacillus subtilis, Bacillus pumilus, Bacillus cereus, Staphylococcus aureus, and Acinetobacter baumanii. It was found to be active against drug resistant Mycobacterium tuberculosis [2].
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