Abstract
A simple, precise, rapid, selective, and economic reversed phase high-performance thin layer chromatography (HPTLC) method has been established for simultaneous analysis of PRG and MC. HPTLC method was developed using on precoated silica gel F254 G60 plates as stationary phase, using methanol:toluene:ammonia (30%) (8:2:0.4 v/v/v) as mobile phase. The plates were scanned at approximately 497 nm for both PRG and MC respectively. In HPTLC method both the drugs were resolved using proposed mobile phase and Rf value was found to be 0.60 for PRG and Rf 0.33 for MC. The method was found to linear in the range 1500-7500 ng/band for PRG, 150-750 ng/ band based for MC respectively. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is important tool for analysis of combined dosage form.
Highlights
Pregabaline (PRG; (3S)-3-(aminomethyl)-5-methylhexanoic acid; (Figure 1a) is a widely used Anticonvulsant and Analgesic for Epilepsy [1]
Methylcobalamine (MC; carbanide;cobalt;[5(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl) oxolan-3-yl]1-[3-[2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3amino-3-oxopropyl)-3,5,8,8,13,15,18,19- octamethyl-2,7,12,17-tetrahydrocorrin-3-yl]propanoylamino]propan-2-yl hydrogen phosphate (Figure 1b) is Derivative form of vitamin B12. It is used for the treatment of peripheral neuropathy, diabetic neuropathy
A mobile phase consisting of methanol:toluene:ammonia (30%) (8:2:0.4, v/v/v) gave good separation of PRG and MC from its matrix
Summary
Pregabaline (PRG; (3S)-3-(aminomethyl)-5-methylhexanoic acid; (Figure 1a) is a widely used Anticonvulsant and Analgesic for Epilepsy [1]. Methylcobalamine (MC; carbanide;cobalt;[5(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl) oxolan-3-yl]1-[3-[2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3amino-3-oxopropyl)-3,5,8,8,13,15,18,19- octamethyl-2,7,12,17-tetrahydrocorrin-3-yl]propanoylamino]propan-2-yl hydrogen phosphate (Figure 1b) is Derivative form of vitamin B12. It is used for the treatment of peripheral neuropathy, diabetic neuropathy
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography & Separation Techniques
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
