Abstract
Androsta-4,16-dien-3-one (androstadienone) and androst-4-en-3-one-17β-ol (testosterone) in healthy human plasma were simultaneously determined under several experimental conditions by gas chromatography—mass spectrometry with high-resolution selected-ion monitoring. Internal standards were [2,2,4,6,6- 2H 5]androstadienone and [2,2,4,6,6- 2H 5]testosterone. Samples were extracted with an Extrelut ® column, purified using Lipidex ® 5000 and converted into hydroxime-trimethylsilyl derivatives for determination. Physiological concentrations of androstadienone and testosterone found in eleven healthy men were 2.05 ± 0.74 and 18.6 ± 4.9 pmol/ml in plasma (mean ± S.D.), respectively. No correlation was observed between these steroid concentrations.
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