Abstract
The determination of the real differential molecular weight distribution requires that the instrumental spreading of the chromatographic band should be taken into account accurately. In other words, the dependence of the instrumental spreading function for an individual substance on the eluent volume should be known under experimental conditions. Current methods of calculations do not deal with a continuous change in the shape of the chromatographic band for individual species (SCBIS) due to the molecular weight. This may be due to inaccurate differential DMWD results. The methods of calculating MWD data from GPC involving the continuous change in SCBIS are presented in this work.
Published Version
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